ID   SFI1_PHYIT              Reviewed;         107 AA.
AC   D0N0J7;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 1.
DT   03-AUG-2022, entry version 37.
DE   RecName: Full=RxLR effector protein SFI1 {ECO:0000303|PubMed:24763622};
DE   AltName: Full=Suppressor of early Flg22-induced immune response 1 {ECO:0000303|PubMed:24763622};
DE   Flags: Precursor;
GN   Name=SFI1 {ECO:0000303|PubMed:24763622}; ORFNames=PITG_04097;
OS   Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=403677;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=T30-4;
RX   PubMed=19741609; DOI=10.1038/nature08358;
RG   The Broad Institute Genome Sequencing Platform;
RA   Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA   Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA   Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA   Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA   Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA   Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA   Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA   Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA   Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA   Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA   Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA   Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA   Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA   Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA   Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA   Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA   Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA   Nusbaum C.;
RT   "Genome sequence and analysis of the Irish potato famine pathogen
RT   Phytophthora infestans.";
RL   Nature 461:393-398(2009).
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=24763622; DOI=10.1371/journal.ppat.1004057;
RA   Zheng X., McLellan H., Fraiture M., Liu X., Boevink P.C., Gilroy E.M.,
RA   Chen Y., Kandel K., Sessa G., Birch P.R., Brunner F.;
RT   "Functionally redundant RXLR effectors from Phytophthora infestans act at
RT   different steps to suppress early flg22-triggered immunity.";
RL   PLoS Pathog. 10:E1004057-E1004057(2014).
CC   -!- FUNCTION: Effector that suppresses flg22-induced post-translational MAP
CC       kinase activation both tomato and Arabidopsis. The perception of highly
CC       conserved pathogen- or microbe-associated molecular patterns
CC       (PAMPs/MAMPs), such as flg22, triggers converging signaling pathways
CC       recruiting MAP kinase cascades and inducing transcriptional re-
CC       programming, yielding a generic antimicrobial response.
CC       {ECO:0000269|PubMed:24763622}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:24763622}. Host
CC       nucleus, host nucleolus {ECO:0000269|PubMed:24763622}. Note=The nuclear
CC       localization of SFI1 is required for suppression of MAMP-triggered
CC       immunity (MTI) signaling in Arabidopsis, and for enhancing P.infestans
CC       colonization of N.benthamiana. {ECO:0000269|PubMed:24763622}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:24763622}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; DS028122; EEY67160.1; -; Genomic_DNA.
DR   RefSeq; XP_002905808.1; XM_002905762.1.
DR   AlphaFoldDB; D0N0J7; -.
DR   EnsemblProtists; PITG_04097T0; PITG_04097T0; PITG_04097.
DR   GeneID; 9479514; -.
DR   KEGG; pif:PITG_04097; -.
DR   VEuPathDB; FungiDB:PITG_04097; -.
DR   eggNOG; ENOG502RGUF; Eukaryota.
DR   HOGENOM; CLU_158959_0_0_1; -.
DR   InParanoid; D0N0J7; -.
DR   OMA; IAHTTNK; -.
DR   OrthoDB; 1628005at2759; -.
DR   PHI-base; PHI:4201; -.
DR   Proteomes; UP000006643; Partially assembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
PE   3: Inferred from homology;
KW   Host nucleus; Reference proteome; Secreted; Signal; Virulence.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..107
FT                   /note="RxLR effector protein SFI1"
FT                   /id="PRO_5003011565"
FT   MOTIF           40..58
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:24763622"
SQ   SEQUENCE   107 AA;  11675 MW;  E4D746D42EF8C712 CRC64;
     MRSIFYVALA FAVLARSSSV EAFPNPKEPQ LLSKASPDKR SLRVEGQEVV QGGTLDGNGG
     VWKAIAHTTN KIVKKPEIDV SKLIDVAKKA KKVKKLKNLM KLKKSSS