SFI3_PHYIT
ID SFI3_PHYIT Reviewed; 127 AA.
AC D0N6D2;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 1.
DT 03-AUG-2022, entry version 41.
DE RecName: Full=RxLR effector protein SFI3 {ECO:0000303|PubMed:24763622};
DE AltName: Full=Suppressor of early Flg22-induced immune response 3 {ECO:0000303|PubMed:24763622};
DE Flags: Precursor;
GN Name=SFI3 {ECO:0000303|PubMed:24763622};
GN Synonyms=PexRD16 {ECO:0000303|PubMed:19794118}; ORFNames=PITG_06087;
OS Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=403677;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T30-4;
RX PubMed=19741609; DOI=10.1038/nature08358;
RG The Broad Institute Genome Sequencing Platform;
RA Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA Nusbaum C.;
RT "Genome sequence and analysis of the Irish potato famine pathogen
RT Phytophthora infestans.";
RL Nature 461:393-398(2009).
RN [2]
RP IDENTIFICATION, AND DOMAIN.
RX PubMed=19794118; DOI=10.1105/tpc.109.068247;
RA Oh S.K., Young C., Lee M., Oliva R., Bozkurt T.O., Cano L.M., Win J.,
RA Bos J.I., Liu H.Y., van Damme M., Morgan W., Choi D., Van der Vossen E.A.,
RA Vleeshouwers V.G., Kamoun S.;
RT "In planta expression screens of Phytophthora infestans RXLR effectors
RT reveal diverse phenotypes, including activation of the Solanum
RT bulbocastanum disease resistance protein Rpi-blb2.";
RL Plant Cell 21:2928-2947(2009).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=24763622; DOI=10.1371/journal.ppat.1004057;
RA Zheng X., McLellan H., Fraiture M., Liu X., Boevink P.C., Gilroy E.M.,
RA Chen Y., Kandel K., Sessa G., Birch P.R., Brunner F.;
RT "Functionally redundant RXLR effectors from Phytophthora infestans act at
RT different steps to suppress early flg22-triggered immunity.";
RL PLoS Pathog. 10:E1004057-E1004057(2014).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 49-127, SUBUNIT, FUNCTION,
RP SUBCELLULAR LOCATION, INTERACTION WITH HOST UBK, AND MUTAGENESIS OF LEU-86;
RP LEU-100 AND LEU-103.
RX PubMed=30536576; DOI=10.1111/nph.15635;
RA He Q., McLellan H., Hughes R.K., Boevink P.C., Armstrong M., Lu Y.,
RA Banfield M.J., Tian Z., Birch P.R.J.;
RT "Phytophthora infestans effector SFI3 targets potato UBK to suppress early
RT immune transcriptional responses.";
RL New Phytol. 222:438-454(2019).
CC -!- FUNCTION: Effector that suppresses flg22-induced post-translational MAP
CC kinase activation in potato and tomato, but not in Arabidopsis. The
CC perception of highly conserved pathogen- or microbe-associated
CC molecular patterns (PAMPs/MAMPs), such as flg22, triggers converging
CC signaling pathways recruiting MAP kinase cascades and inducing
CC transcriptional re-programming, yielding a generic antimicrobial
CC response (PubMed:24763622, PubMed:30536576). Does not suppress
CC programmed cell death triggered by the P.infestans elicitin infestin-1
CC (INF1), or by co-expression of tomato Cf4 with Cladosporium fulvum Avr4
CC (PubMed:30536576). Suppresses early pattern-triggered immunity (PTI)
CC via interaction with the U-box-kinase protein UBK, a positive regulator
CC of specific PTI pathways in both potato and Nicotiana benthamiana
CC (PubMed:30536576). {ECO:0000269|PubMed:24763622,
CC ECO:0000269|PubMed:30536576}.
CC -!- SUBUNIT: Forms an unusual trans-homodimer (PubMed:30536576). Interacts
CC with host UBK (PubMed:30536576). {ECO:0000269|PubMed:30536576}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:24763622}. Host
CC nucleus, host nucleolus {ECO:0000269|PubMed:24763622}. Host nucleus
CC {ECO:0000269|PubMed:30536576}. Note=Forms a ring around the nucleolus.
CC {ECO:0000269|PubMed:24763622}.
CC -!- DOMAIN: The WY-domain is involved in the homodimerization leading to a
CC novel trans WY-domain configuration in which the Trp and Tyr residues
CC are derived from separate monomers. {ECO:0000269|PubMed:30536576}.
CC -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC phosphate. {ECO:0000305|PubMed:19794118}.
CC -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR EMBL; DS028126; EEY70623.1; -; Genomic_DNA.
DR RefSeq; XP_002998277.1; XM_002998231.1.
DR PDB; 6GU1; X-ray; 1.70 A; A/B=49-127.
DR PDBsum; 6GU1; -.
DR AlphaFoldDB; D0N6D2; -.
DR SMR; D0N6D2; -.
DR EnsemblProtists; PITG_06087T0; PITG_06087T0; PITG_06087.
DR GeneID; 9472246; -.
DR KEGG; pif:PITG_06087; -.
DR VEuPathDB; FungiDB:PITG_06087; -.
DR eggNOG; ENOG502RFDT; Eukaryota.
DR HOGENOM; CLU_1974887_0_0_1; -.
DR InParanoid; D0N6D2; -.
DR OMA; FYTAMEK; -.
DR OrthoDB; 1648902at2759; -.
DR PHI-base; PHI:4202; -.
DR Proteomes; UP000006643; Partially assembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW 3D-structure; Host nucleus; Reference proteome; Secreted; Signal;
KW Virulence.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..127
FT /note="RxLR effector protein SFI3"
FT /id="PRO_5003012132"
FT REGION 72..107
FT /note="WY-domain"
FT /evidence="ECO:0000305|PubMed:30536576"
FT MOTIF 40..62
FT /note="RxLR-dEER"
FT /evidence="ECO:0000305|PubMed:19794118"
FT MUTAGEN 86
FT /note="L->D: Disrupts homooligomerization, prevents
FT association with UBK, impairs nucleolar localization and
FT accumulates in the nucleoplasm with cytoplasmic background;
FT when associated with D-100 or D-103."
FT /evidence="ECO:0000269|PubMed:30536576"
FT MUTAGEN 100
FT /note="L->D: Disrupts homooligomerization, prevents
FT association with UBK, impairs nucleolar localization and
FT accumulates in the nucleoplasm with cytoplasmic background;
FT when associated with D-86."
FT /evidence="ECO:0000269|PubMed:30536576"
FT MUTAGEN 103
FT /note="L->D: Disrupts homooligomerization, prevents
FT association with UBK, impairs nucleolar localization and
FT accumulates in the nucleoplasm with cytoplasmic background;
FT when associated with D-86."
FT /evidence="ECO:0000269|PubMed:30536576"
FT HELIX 64..74
FT /evidence="ECO:0007829|PDB:6GU1"
FT HELIX 79..116
FT /evidence="ECO:0007829|PDB:6GU1"
SQ SEQUENCE 127 AA; 14388 MW; 585EB6F34B155692 CRC64;
MRFLLVAVVA MMALVSSSTA AVAETSNDIN TMNNNQEFAR SLRNTEERSI AAILAEAGEE
DRAAWRINYR AWYKAKLTPT QVKTVLGVSQ AEMNNVAKQL QRLYLGYYSF YTAMEKKKEE
KKRLATP
