SFI5_PHYIT
ID SFI5_PHYIT Reviewed; 241 AA.
AC D0NMF6;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 1.
DT 03-AUG-2022, entry version 37.
DE RecName: Full=RxLR effector protein SFI5 {ECO:0000303|PubMed:24763622};
DE AltName: Full=Suppressor of early Flg22-induced immune response 5 {ECO:0000303|PubMed:24763622};
DE Flags: Precursor;
GN Name=SFI5 {ECO:0000303|PubMed:24763622};
GN Synonyms=PexRD27 {ECO:0000303|PubMed:19794118}; ORFNames=PITG_13628;
OS Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=403677;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T30-4;
RX PubMed=19741609; DOI=10.1038/nature08358;
RG The Broad Institute Genome Sequencing Platform;
RA Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA Nusbaum C.;
RT "Genome sequence and analysis of the Irish potato famine pathogen
RT Phytophthora infestans.";
RL Nature 461:393-398(2009).
RN [2]
RP INDUCTION.
RX PubMed=19794118; DOI=10.1105/tpc.109.068247;
RA Oh S.K., Young C., Lee M., Oliva R., Bozkurt T.O., Cano L.M., Win J.,
RA Bos J.I., Liu H.Y., van Damme M., Morgan W., Choi D., Van der Vossen E.A.,
RA Vleeshouwers V.G., Kamoun S.;
RT "In planta expression screens of Phytophthora infestans RXLR effectors
RT reveal diverse phenotypes, including activation of the Solanum
RT bulbocastanum disease resistance protein Rpi-blb2.";
RL Plant Cell 21:2928-2947(2009).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=24763622; DOI=10.1371/journal.ppat.1004057;
RA Zheng X., McLellan H., Fraiture M., Liu X., Boevink P.C., Gilroy E.M.,
RA Chen Y., Kandel K., Sessa G., Birch P.R., Brunner F.;
RT "Functionally redundant RXLR effectors from Phytophthora infestans act at
RT different steps to suppress early flg22-triggered immunity.";
RL PLoS Pathog. 10:E1004057-E1004057(2014).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH CALMODULIN.
RX PubMed=29932222; DOI=10.1111/nph.15250;
RA Zheng X., Wagener N., McLellan H., Boevink P.C., Hua C., Birch P.R.J.,
RA Brunner F.;
RT "Phytophthora infestans RXLR effector SFI5 requires association with
RT calmodulin for PTI/MTI suppressing activity.";
RL New Phytol. 219:1433-1446(2018).
CC -!- FUNCTION: Effector that suppresses flg22-induced post-translational MAP
CC kinase activation in tomato but not in Arabidopsis. The perception of
CC highly conserved pathogen- or microbe-associated molecular patterns
CC (PAMPs/MAMPs), such as flg22, triggers converging signaling pathways
CC recruiting MAP kinase cascades and inducing transcriptional re-
CC programming, yielding a generic antimicrobial response.
CC {ECO:0000269|PubMed:24763622}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:24763622}. Host cell
CC membrane {ECO:0000269|PubMed:24763622}.
CC -!- INDUCTION: Expression is induced during host plant infection.
CC {ECO:0000269|PubMed:19794118}.
CC -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC phosphate. {ECO:0000305|PubMed:24763622}.
CC -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR EMBL; DS028146; EEY60877.1; -; Genomic_DNA.
DR RefSeq; XP_002899823.1; XM_002899777.1.
DR AlphaFoldDB; D0NMF6; -.
DR SMR; D0NMF6; -.
DR EnsemblProtists; PITG_13628T0; PITG_13628T0; PITG_13628.
DR GeneID; 9462261; -.
DR KEGG; pif:PITG_13628; -.
DR VEuPathDB; FungiDB:PITG_13628; -.
DR eggNOG; ENOG502RG9H; Eukaryota.
DR HOGENOM; CLU_1201884_0_0_1; -.
DR InParanoid; D0NMF6; -.
DR OMA; NAYAFEL; -.
DR OrthoDB; 1507433at2759; -.
DR PHI-base; PHI:4204; -.
DR Proteomes; UP000006643; Partially assembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR InterPro; IPR040691; PexRD2_WYL.
DR Pfam; PF18488; WYL_3; 1.
PE 1: Evidence at protein level;
KW Host cell membrane; Host membrane; Membrane; Reference proteome; Secreted;
KW Signal; Virulence.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..241
FT /note="RxLR effector protein SFI5"
FT /id="PRO_5003012222"
FT MOTIF 44..62
FT /note="RxLR-dEER"
FT /evidence="ECO:0000305|PubMed:24763622"
SQ SEQUENCE 241 AA; 27327 MW; 7E28B1679484B455 CRC64;
MLRQARPLVV LIAVTFLVAS EVFSMALASD QNSNVASITS QVQRLLRTHH ATIKVNADSE
ERFLTEPPLT TDEMMAMMKA GKSKNAYAFE LGIAGQMADF INSGLPDIET FKKTPEFQKY
EFYMNFLNDM RKDDDYKPLV EMIKKNKGET EAFKTLLVKV EDSVSKKKAS PSAIVKLDPL
NREQAIVEKI ELALKKNQAL NKNKASLETI EHTVRMAAKS KPSTWKIFKI ISRLKKLKLK
R
