SFI8_PHYIT
ID SFI8_PHYIT Reviewed; 100 AA.
AC D0P1B2;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 1.
DT 03-AUG-2022, entry version 36.
DE RecName: Full=RxLR effector protein SFI8 {ECO:0000303|PubMed:24763622};
DE AltName: Full=Suppressor of early Flg22-induced immune response 8 {ECO:0000303|PubMed:24763622};
DE Flags: Precursor;
GN Name=SFI8 {ECO:0000303|PubMed:24763622}; ORFNames=PITG_20303;
OS Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=403677;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T30-4;
RX PubMed=19741609; DOI=10.1038/nature08358;
RG The Broad Institute Genome Sequencing Platform;
RA Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA Nusbaum C.;
RT "Genome sequence and analysis of the Irish potato famine pathogen
RT Phytophthora infestans.";
RL Nature 461:393-398(2009).
RN [2]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=24763622; DOI=10.1371/journal.ppat.1004057;
RA Zheng X., McLellan H., Fraiture M., Liu X., Boevink P.C., Gilroy E.M.,
RA Chen Y., Kandel K., Sessa G., Birch P.R., Brunner F.;
RT "Functionally redundant RXLR effectors from Phytophthora infestans act at
RT different steps to suppress early flg22-triggered immunity.";
RL PLoS Pathog. 10:E1004057-E1004057(2014).
RN [3]
RP INTERACTION WITH HOST CALMODULIN, AND FUNCTION.
RX PubMed=30984224; DOI=10.3389/fpls.2019.00374;
RA Naveed Z.A., Bibi S., Ali G.S.;
RT "The Phytophthora RXLR Effector Avrblb2 Modulates Plant Immunity by
RT Interfering With Ca2+ Signaling Pathway.";
RL Front. Plant Sci. 10:374-374(2019).
CC -!- FUNCTION: Effector that suppresses flg22-induced post-translational MAP
CC kinase activation both tomato and Arabidopsis. The perception of highly
CC conserved pathogen- or microbe-associated molecular patterns
CC (PAMPs/MAMPs), such as flg22, triggers converging signaling pathways
CC recruiting MAP kinase cascades and inducing transcriptional re-
CC programming, yielding a generic antimicrobial response
CC (PubMed:24763622). Associates with calmodulin to interfere with plant
CC defense-associated calcium signaling in hosts (PubMed:30984224).
CC {ECO:0000269|PubMed:24763622, ECO:0000269|PubMed:30984224}.
CC -!- SUBUNIT: Interacts with the host calmodulin.
CC {ECO:0000269|PubMed:30984224}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:24763622}. Host
CC nucleus {ECO:0000269|PubMed:24763622}. Host cytoplasm
CC {ECO:0000269|PubMed:24763622}.
CC -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC phosphate. {ECO:0000305|PubMed:24763622}.
CC -!- DOMAIN: The amino acids 78-82 in the C-terminal region of are important
CC for binding to host calmodulin. {ECO:0000250|UniProtKB:D0P1A8}.
CC -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR EMBL; DS028242; EEY54138.1; -; Genomic_DNA.
DR RefSeq; XP_002895922.1; XM_002895876.1.
DR AlphaFoldDB; D0P1B2; -.
DR EnsemblProtists; PITG_20303T0; PITG_20303T0; PITG_20303.
DR GeneID; 9469961; -.
DR KEGG; pif:PITG_20303; -.
DR VEuPathDB; FungiDB:PITG_20303; -.
DR HOGENOM; CLU_158959_0_0_1; -.
DR InParanoid; D0P1B2; -.
DR OMA; ANXALAI; -.
DR OrthoDB; 1628005at2759; -.
DR PHI-base; PHI:4207; -.
DR Proteomes; UP000006643; Partially assembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Host cytoplasm; Host nucleus; Reference proteome; Secreted; Signal;
KW Virulence.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..100
FT /note="RxLR effector protein SFI8"
FT /id="PRO_5003013855"
FT MOTIF 43..57
FT /note="RxLR-dEER"
FT /evidence="ECO:0000305|PubMed:24763622"
FT MOTIF 78..82
FT /note="Calmodulin-binding motif"
FT /evidence="ECO:0000250|UniProtKB:D0P1A8"
SQ SEQUENCE 100 AA; 10752 MW; 0CF8A85C9DA66195 CRC64;
MRSILYAVLA FAVLARSSAV AAFPIPDESR PLSKTSPDTG ATRSLRVEAQ EVIQSGRGDG
YGGFWKNVFP STNKIIKKPD IKISKLIAAA KKAKAKMTKS
