ID   SFIF_SEGFL              Reviewed;          18 AA.
AC   P61094;
DT   26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2004, sequence version 1.
DT   25-MAY-2022, entry version 41.
DE   RecName: Full=Mu-segestritoxin-Sf1i {ECO:0000305};
DE            Short=Mu-SGTX-Sf1i {ECO:0000305};
DE   AltName: Full=Toxin F5.5 {ECO:0000303|PubMed:11689233};
DE   Flags: Fragment;
OS   Segestria florentina (Tube-web spider) (Segestria gracilis).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Haplogynae; Dysderoidea; Segestriidae; Segestria.
OX   NCBI_TaxID=31925;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND
RP   TOXIC DOSE.
RC   TISSUE=Venom;
RX   PubMed=11689233; DOI=10.1016/s0041-0101(01)00181-7;
RA   Lipkin A., Kozlov S., Nosyreva E., Blake A., Windass J.D., Grishin E.;
RT   "Novel insecticidal toxins from the venom of the spider Segestria
RT   florentina.";
RL   Toxicon 40:125-130(2002).
CC   -!- FUNCTION: Insecticidal toxin. Causes flaccid paralysis followed by
CC       death when injected into Heliothis virescens larvae. Does not induce
CC       any toxic effects when injected intravenously into adult mice at a dose
CC       of 1.1 mg/kg body weight. {ECO:0000269|PubMed:11689233}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11689233}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:11689233}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000250|UniProtKB:P61095}.
CC   -!- PTM: Contains 4 disulfide bonds. {ECO:0000250|UniProtKB:P61095}.
CC   -!- MASS SPECTROMETRY: Mass=5159; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:11689233};
CC   -!- TOXIC DOSE: LD(50) is 4 mg/kg on H.virescens larvae.
CC       {ECO:0000269|PubMed:11689233}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 16 (SFI) family. {ECO:0000305}.
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DR   AlphaFoldDB; P61094; -.
DR   ArachnoServer; AS000124; mu-segestritoxin-Sf1i (N-terminal fragment).
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR012633; Toxin_28.
DR   Pfam; PF08115; Toxin_28; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Secreted; Toxin.
FT   CHAIN           1..>18
FT                   /note="Mu-segestritoxin-Sf1i"
FT                   /evidence="ECO:0000269|PubMed:11689233"
FT                   /id="PRO_0000087615"
FT   UNSURE          15
FT                   /evidence="ECO:0000305|PubMed:11689233"
FT   NON_TER         18
FT                   /evidence="ECO:0000305|PubMed:11689233"
SQ   SEQUENCE   18 AA;  2095 MW;  B0F43D60A1573120 CRC64;
     AECMVDETVC YIHNHNNC