SFK1_YEAST
ID SFK1_YEAST Reviewed; 353 AA.
AC P35735; D6VXN6;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Protein SFK1;
DE AltName: Full=Suppressor of four kinase protein 1;
GN Name=SFK1; OrderedLocusNames=YKL051W; ORFNames=YKL303;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091862; DOI=10.1002/yea.320100008;
RA Rasmussen S.W.;
RT "Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and
RT TOA2 genes, an open reading frame (ORF) similar to a translationally
RT controlled tumour protein, one ORF containing motifs also found in plant
RT storage proteins and 13 ORFs with weak or no homology to known proteins.";
RL Yeast 10:S63-S68(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=12015967; DOI=10.1016/s1534-5807(02)00168-5;
RA Audhya A., Emr S.D.;
RT "Stt4 PI 4-kinase localizes to the plasma membrane and functions in the
RT Pkc1-mediated MAP kinase cascade.";
RL Dev. Cell 2:593-605(2002).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-208, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: May control the production of phosphatidylinositol 4-
CC phosphate (PI4P) by the STT4 PI 4-kinase. Mediates proper localization
CC of STT4 to the plasma membrane. {ECO:0000269|PubMed:12015967}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12015967,
CC ECO:0000269|PubMed:14562095}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:12015967, ECO:0000269|PubMed:14562095}.
CC -!- SIMILARITY: Belongs to the SFK1 family. {ECO:0000305}.
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DR EMBL; X75781; CAA53425.1; -; Genomic_DNA.
DR EMBL; Z28051; CAA81886.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09106.1; -; Genomic_DNA.
DR PIR; S37873; S37873.
DR RefSeq; NP_012873.1; NM_001179617.1.
DR AlphaFoldDB; P35735; -.
DR BioGRID; 34082; 110.
DR DIP; DIP-5309N; -.
DR IntAct; P35735; 47.
DR MINT; P35735; -.
DR STRING; 4932.YKL051W; -.
DR iPTMnet; P35735; -.
DR MaxQB; P35735; -.
DR PaxDb; P35735; -.
DR PRIDE; P35735; -.
DR EnsemblFungi; YKL051W_mRNA; YKL051W; YKL051W.
DR GeneID; 853815; -.
DR KEGG; sce:YKL051W; -.
DR SGD; S000001534; SFK1.
DR VEuPathDB; FungiDB:YKL051W; -.
DR eggNOG; ENOG502RZQS; Eukaryota.
DR HOGENOM; CLU_050573_1_0_1; -.
DR InParanoid; P35735; -.
DR OMA; HRVHIAM; -.
DR BioCyc; YEAST:G3O-31852-MON; -.
DR PRO; PR:P35735; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P35735; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0030036; P:actin cytoskeleton organization; IGI:SGD.
DR GO; GO:0048017; P:inositol lipid-mediated signaling; IGI:SGD.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0061093; P:negative regulation of phospholipid translocation; IMP:SGD.
DR GO; GO:0007033; P:vacuole organization; IGI:SGD.
DR InterPro; IPR019402; Frag1/DRAM/Sfk1.
DR Pfam; PF10277; Frag1; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Lipid biosynthesis; Lipid metabolism; Membrane;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..353
FT /note="Protein SFK1"
FT /id="PRO_0000203179"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 33..62
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 84..130
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 152..160
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 182..231
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 254..263
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 285..353
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 208
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 353 AA; 40463 MW; BB66ED73F1181331 CRC64;
MIQFKSPGNW LFIVPWIAFI PWYGMLIAML ICWASQGHPI YWFMHSEQFP VYISDIGATN
LRPLFISCAG WQGLGYVITV ALEFFQRSGY LPFQLKKKDP SISDSTSYAE KLHSGKYLMP
PYYTKDERNL IFAAFVLGSI GELALLFSSI FSTALYHRVH IAMVSVFVVF MFLSTCCLIA
EYFLMGRHYA SVHPLASPHF NPQSSEKSFN QDYNTVDELP WYKWKGHVWN KFTISATLKV
IWLTLAVVWA ICFGAINDRS KSACFEWLLA FWFGIIFMIL SADFYLGGRY RQSRYFNHVE
SFSGYYKYDK ALGLYHSEDV LPSDDNAGVI ATETASSNIY NNSSSNESIQ VVV
