SFL2_CANAL
ID SFL2_CANAL Reviewed; 714 AA.
AC Q5AK51; A0A1D8PP22; Q5AKL3;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 19-MAR-2014, sequence version 2.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=Transcription factor SFL2;
GN Name=SFL2; OrderedLocusNames=CAALFM_C504830WA;
GN ORFNames=CaO19.11452, CaO19.3969;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP INDUCTION, FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20023067; DOI=10.1128/ec.00291-09;
RA Spiering M.J., Moran G.P., Chauvel M., Maccallum D.M., Higgins J.,
RA Hokamp K., Yeomans T., d'Enfert C., Coleman D.C., Sullivan D.J.;
RT "Comparative transcript profiling of Candida albicans and Candida
RT dubliniensis identifies SFL2, a C. albicans gene required for virulence in
RT a reconstituted epithelial infection model.";
RL Eukaryot. Cell 9:251-265(2010).
RN [5]
RP INDUCTION, FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=21205158; DOI=10.1111/j.1567-1364.2010.00710.x;
RA Song W., Wang H., Chen J.;
RT "Candida albicans Sfl2, a temperature-induced transcriptional regulator, is
RT required for virulence in a murine gastrointestinal infection model.";
RL FEMS Yeast Res. 11:209-222(2011).
RN [6]
RP FUNCTION.
RX PubMed=23049891; DOI=10.1371/journal.pone.0045912;
RA Chauvel M., Nesseir A., Cabral V., Znaidi S., Goyard S.,
RA Bachellier-Bassi S., Firon A., Legrand M., Diogo D., Naulleau C.,
RA Rossignol T., d'Enfert C.;
RT "A versatile overexpression strategy in the pathogenic yeast Candida
RT albicans: identification of regulators of morphogenesis and fitness.";
RL PLoS ONE 7:E45912-E45912(2012).
RN [7]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=23966855; DOI=10.1371/journal.ppat.1003519;
RA Znaidi S., Nesseir A., Chauvel M., Rossignol T., d'Enfert C.;
RT "A comprehensive functional portrait of two heat shock factor-type
RT transcriptional regulators involved in Candida albicans morphogenesis and
RT virulence.";
RL PLoS Pathog. 9:E1003519-E1003519(2013).
CC -!- FUNCTION: Transcription factor that plays a role of activator of
CC filamentous growth and which is involved in invasive growth at a high
CC temperature. Required for human oral epithelium colonization and
CC damage. Promotes filamentous growth in EFG1- and FLO8-dependent
CC manners. Antagonizes functions of SFL1. {ECO:0000269|PubMed:20023067,
CC ECO:0000269|PubMed:21205158, ECO:0000269|PubMed:23049891,
CC ECO:0000269|PubMed:23966855}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21205158}.
CC Note=Localizes to the nucleus in both yeast and hyphal cells.
CC -!- INDUCTION: Induced at high temperature and during human oral epithelium
CC infection. {ECO:0000269|PubMed:20023067, ECO:0000269|PubMed:21205158}.
CC -!- DISRUPTION PHENOTYPE: Fails to undergo filamentation under a range of
CC hypha-inducing conditions and attenuates the virulence towards
CC reconstituted human oral epithelium and in a mouse model of
CC gastrointestinal colonization and dissemination.
CC {ECO:0000269|PubMed:20023067}.
CC -!- SIMILARITY: Belongs to the HSF family. {ECO:0000305}.
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DR EMBL; CP017627; AOW29887.1; -; Genomic_DNA.
DR RefSeq; XP_721878.2; XM_716785.2.
DR AlphaFoldDB; Q5AK51; -.
DR SMR; Q5AK51; -.
DR BioGRID; 1219336; 2.
DR STRING; 237561.Q5AK51; -.
DR PRIDE; Q5AK51; -.
DR GeneID; 3636434; -.
DR KEGG; cal:CAALFM_C504830WA; -.
DR CGD; CAL0000195882; SFL2.
DR VEuPathDB; FungiDB:C5_04830W_A; -.
DR eggNOG; KOG0627; Eukaryota.
DR HOGENOM; CLU_015115_0_0_1; -.
DR InParanoid; Q5AK51; -.
DR OrthoDB; 1154048at2759; -.
DR PHI-base; PHI:2569; -.
DR PRO; PR:Q5AK51; -.
DR Proteomes; UP000000559; Chromosome 5.
DR GO; GO:0000228; C:nuclear chromosome; IDA:CGD.
DR GO; GO:0005634; C:nucleus; IDA:CGD.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:CGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0036244; P:cellular response to neutral pH; IMP:CGD.
DR GO; GO:0030447; P:filamentous growth; IMP:CGD.
DR GO; GO:0044182; P:filamentous growth of a population of unicellular organisms; IMP:CGD.
DR GO; GO:0036180; P:filamentous growth of a population of unicellular organisms in response to biotic stimulus; IMP:CGD.
DR GO; GO:0036178; P:filamentous growth of a population of unicellular organisms in response to neutral pH; IMP:CGD.
DR GO; GO:0000128; P:flocculation; IGI:CGD.
DR GO; GO:1900429; P:negative regulation of filamentous growth of a population of unicellular organisms; IMP:CGD.
DR GO; GO:0060257; P:negative regulation of flocculation; IMP:CGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:CGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR000232; HSF_DNA-bd.
DR InterPro; IPR027725; HSF_fam.
DR InterPro; IPR027722; Sfl1.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10015; PTHR10015; 1.
DR PANTHER; PTHR10015:SF388; PTHR10015:SF388; 1.
DR Pfam; PF00447; HSF_DNA-bind; 1.
DR PRINTS; PR00056; HSFDOMAIN.
DR SMART; SM00415; HSF; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00434; HSF_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Virulence.
FT CHAIN 1..714
FT /note="Transcription factor SFL2"
FT /id="PRO_0000425615"
FT DNA_BIND 15..134
FT /evidence="ECO:0000250"
FT REGION 187..467
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 565..658
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 670..714
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 190..207
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 222..236
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 243..284
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 285..300
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 301..320
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 354..395
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 406..467
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 670..702
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 714 AA; 78220 MW; F47A53C3653E70E2 CRC64;
MSKKNPGDPR VKKNAFVHKL YTMLHDPALS HLIWWTNRNG EQNTFALCPG KEFADCLTQY
FKHGNVASFV RQLHMYGFHK VSDPLPITYP PNGNNNNNNN NKEVPPVWEF KHSSGRFKRG
DETSLVYIKR RSSSNHTSGS TIYAEPIYNP LLTNPYNQPQ FMYQDYSMGP PVEVQQFYNY
QNQPLMYYQQ PPGQVPPPPP PSLPPHQQPE MALQYHQYNN YQQPPPAPPA PPPQPGNVLF
GYQQVAPPQQ PGSTVQQIPA NAPPTLDQTQ PLSYTPQLEY QQQQYPQPPL PPPPPQLQLQ
TSPGIPKVND NLSRPSPNEQ HLQFRKIWPD ENNEANSKPR NPSLMFDPLL RVNSEGSPKT
QPNHSVSLLN NEVRSESSTS SSSTTVTSTA LPPPSAIDRS VSLVGGSPFD LSSRMNNQQS
FNRTPSISTP PTQNGRLPKI SSPLIPSNTE SQSPTMVTGT DNDVGVTSKG STSIISVSKK
PSVFSNSLQE RLRPSMFEYH PIGNNLSKDV LTIPKISTNS NSGSRTTSQS SMSSASALSS
KKSSLSSISS THGNLSILNS TNYRSTSVGG SGSGPFSTST STSTTSPTLS SLLHHPQHEP
QNSTIANGTS IRSSISSSQS ISSPPLTTTT TTTTTDQRQL SNSPISRQQQ QQPNNNTNKK
VSVTSLLLPS HSQSQGYAQP LYKSSVIAEE ENSTTSSGGG YRSKSDDDTD NMNK
