ID   SFP3_BOVIN              Reviewed;         140 AA.
AC   P04557; Q9TUE5;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   27-JAN-2003, sequence version 2.
DT   03-AUG-2022, entry version 135.
DE   RecName: Full=Seminal plasma protein A3;
DE   AltName: Full=BSP-A3;
DE   Flags: Precursor;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10377061; DOI=10.1095/biolreprod61.1.288;
RA   Salois D., Menard M., Paquette Y., Manjunath P.;
RT   "Complementary deoxyribonucleic acid cloning and tissue expression of BSP-
RT   A3 and BSP-30-kDa: phosphatidylcholine and heparin-binding proteins of
RT   bovine seminal plasma.";
RL   Biol. Reprod. 61:288-297(1999).
RN   [2]
RP   PROTEIN SEQUENCE OF 26-140.
RC   TISSUE=Seminal plasma;
RX   PubMed=3606570; DOI=10.1042/bj2430195;
RA   Seidah N.G., Manjunath P., Rochemont J., Sairam M.R., Chretien M.;
RT   "Complete amino acid sequence of BSP-A3 from bovine seminal plasma.
RT   Homology to PDC-109 and to the collagen-binding domain of fibronectin.";
RL   Biochem. J. 243:195-203(1987).
CC   -!- FUNCTION: The BSP-A proteins from seminal plasma exhibit both
CC       simulatory and inhibitory actions on the release of pituitary
CC       gonadotropins. The exact function of these proteins is not known.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the seminal plasma protein family.
CC       {ECO:0000305}.
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DR   EMBL; AF055981; AAF04405.1; -; mRNA.
DR   RefSeq; NP_777265.1; NM_174840.1.
DR   AlphaFoldDB; P04557; -.
DR   SMR; P04557; -.
DR   STRING; 9913.ENSBTAP00000005069; -.
DR   PaxDb; P04557; -.
DR   Ensembl; ENSBTAT00000005069; ENSBTAP00000005069; ENSBTAG00000003886.
DR   GeneID; 317695; -.
DR   KEGG; bta:317695; -.
DR   CTD; 317695; -.
DR   VEuPathDB; HostDB:ENSBTAG00000003886; -.
DR   eggNOG; KOG1565; Eukaryota.
DR   GeneTree; ENSGT00940000164580; -.
DR   HOGENOM; CLU_126630_0_0_1; -.
DR   InParanoid; P04557; -.
DR   OMA; KNDYAKC; -.
DR   OrthoDB; 1429125at2759; -.
DR   TreeFam; TF343543; -.
DR   Proteomes; UP000009136; Chromosome 18.
DR   Bgee; ENSBTAG00000003886; Expressed in oviduct epithelium and 15 other tissues.
DR   GO; GO:0009986; C:cell surface; IBA:GO_Central.
DR   GO; GO:0005615; C:extracellular space; IDA:CAFA.
DR   GO; GO:0008201; F:heparin binding; IBA:GO_Central.
DR   GO; GO:0033700; P:phospholipid efflux; IDA:CAFA.
DR   GO; GO:1902492; P:positive regulation of sperm capacitation; IDA:CAFA.
DR   GO; GO:0007338; P:single fertilization; IEA:UniProtKB-KW.
DR   GO; GO:0048240; P:sperm capacitation; IBA:GO_Central.
DR   CDD; cd00062; FN2; 2.
DR   Gene3D; 2.10.10.10; -; 2.
DR   InterPro; IPR000562; FN_type2_dom.
DR   InterPro; IPR036943; FN_type2_sf.
DR   InterPro; IPR013806; Kringle-like.
DR   InterPro; IPR016356; Seminal_plasma_PDC-109-like.
DR   Pfam; PF00040; fn2; 2.
DR   PIRSF; PIRSF002541; Seminal_plasma_PDC-109; 1.
DR   SMART; SM00059; FN2; 2.
DR   SUPFAM; SSF57440; SSF57440; 2.
DR   PROSITE; PS00023; FN2_1; 2.
DR   PROSITE; PS51092; FN2_2; 2.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Fertilization;
KW   Reference proteome; Repeat; Secreted; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000269|PubMed:3606570"
FT   CHAIN           26..140
FT                   /note="Seminal plasma protein A3"
FT                   /id="PRO_0000019232"
FT   DOMAIN          49..93
FT                   /note="Fibronectin type-II 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00479"
FT   DOMAIN          94..140
FT                   /note="Fibronectin type-II 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00479"
FT   DISULFID        54..78
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00479"
FT   DISULFID        68..91
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00479"
FT   DISULFID        99..125
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00479"
FT   DISULFID        113..140
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00479"
FT   CONFLICT        27
FT                   /note="E -> Q (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        115
FT                   /note="I -> K (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   140 AA;  16140 MW;  624475EAD9BE52A6 CRC64;
     MALRLGLFLI WAGVSMFLQL DPVNGDEQLS EDNVILPKEK KDPASGAETK DNKCVFPFIY
     GNKKYFDCTL HGSLFLWCSL DADYTGRWKY CTKNDYAKCV FPFIYEGKSY DTCIIIGSTF
     MNYWCSLSSN YDEDGVWKYC