SFR1_SCHPO
ID SFR1_SCHPO Reviewed; 299 AA.
AC Q9USV1; P78932;
DT 27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Swi5-dependent recombination DNA repair protein 1;
DE AltName: Full=DNA repair protein dds20;
DE AltName: Full=Meiotically up-regulated gene 13 protein;
GN Name=sfr1; Synonyms=dds20, mug13; ORFNames=SPBC28F2.07;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INTERACTION WITH SWI5 AND
RP RHP51.
RX PubMed=14663140; DOI=10.1073/pnas.2632890100;
RA Akamatsu Y., Dziadkowiec D., Ikeguchi M., Shinagawa H., Iwasaki H.;
RT "Two different Swi5-containing protein complexes are involved in mating-
RT type switching and recombination repair in fission yeast.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:15770-15775(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Bashkirov V.I., Khasanov F.K., Savchenko G.V.;
RT "dds20+ is a novel S. pombe gene involved in DNA double-strand break
RT repair.";
RL Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RA Kohnosu A., Niwa O., Yano M., Saitoh S., Katayama T., Nagao K.,
RA Yanagida M.;
RT "S.pombe chromosome II cosmid 1228 sequence.";
RL Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [5]
RP FUNCTION IN MEIOSIS.
RX PubMed=16303567; DOI=10.1016/j.cub.2005.10.038;
RA Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L.,
RA Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P.,
RA Smith G.R., Moreno S.;
RT "A large-scale screen in S. pombe identifies seven novel genes required for
RT critical meiotic events.";
RL Curr. Biol. 15:2056-2062(2005).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Involved in DNA recombination repair and meiotic chromosome
CC segregation. {ECO:0000269|PubMed:14663140,
CC ECO:0000269|PubMed:16303567}.
CC -!- SUBUNIT: Interacts with swi5 and rhp51. {ECO:0000269|PubMed:14663140}.
CC -!- INTERACTION:
CC Q9USV1; P36601: rhp51; NbExp=6; IntAct=EBI-927233, EBI-926960;
CC Q9USV1; Q9UUB7: swi5; NbExp=17; IntAct=EBI-927233, EBI-926902;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the SFR1/MEI5 family. {ECO:0000305}.
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DR EMBL; AB089500; BAC77073.1; -; Genomic_DNA.
DR EMBL; AY275538; AAQ19997.1; -; mRNA.
DR EMBL; D83992; BAA12180.1; -; Genomic_DNA.
DR EMBL; CU329671; CAB57936.1; -; Genomic_DNA.
DR PIR; T40050; T40050.
DR RefSeq; NP_595668.1; NM_001021563.2.
DR PDB; 3VIQ; X-ray; 2.20 A; A/C=181-299.
DR PDBsum; 3VIQ; -.
DR AlphaFoldDB; Q9USV1; -.
DR SMR; Q9USV1; -.
DR BioGRID; 277110; 30.
DR DIP; DIP-29234N; -.
DR IntAct; Q9USV1; 74.
DR STRING; 4896.SPBC28F2.07.1; -.
DR iPTMnet; Q9USV1; -.
DR MaxQB; Q9USV1; -.
DR PaxDb; Q9USV1; -.
DR PRIDE; Q9USV1; -.
DR EnsemblFungi; SPBC28F2.07.1; SPBC28F2.07.1:pep; SPBC28F2.07.
DR GeneID; 2540584; -.
DR KEGG; spo:SPBC28F2.07; -.
DR PomBase; SPBC28F2.07; sfr1.
DR VEuPathDB; FungiDB:SPBC28F2.07; -.
DR eggNOG; ENOG502SAMI; Eukaryota.
DR HOGENOM; CLU_082817_0_0_1; -.
DR InParanoid; Q9USV1; -.
DR OMA; YAEYSID; -.
DR PhylomeDB; Q9USV1; -.
DR PRO; PR:Q9USV1; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; IDA:PomBase.
DR GO; GO:0035861; C:site of double-strand break; IDA:PomBase.
DR GO; GO:0032798; C:Swi5-Sfr1 complex; IDA:PomBase.
DR GO; GO:0001671; F:ATPase activator activity; IDA:PomBase.
DR GO; GO:0003690; F:double-stranded DNA binding; IDA:PomBase.
DR GO; GO:0003697; F:single-stranded DNA binding; IDA:PomBase.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:0000730; P:DNA recombinase assembly; IDA:PomBase.
DR GO; GO:0006310; P:DNA recombination; IBA:GO_Central.
DR GO; GO:1990918; P:double-strand break repair involved in meiotic recombination; IMP:PomBase.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IGI:PomBase.
DR GO; GO:0010772; P:meiotic DNA recombinase assembly involved in reciprocal meiotic recombination; IDA:PomBase.
DR GO; GO:0000709; P:meiotic joint molecule formation; IDA:PomBase.
DR GO; GO:0000708; P:meiotic strand invasion; IDA:PomBase.
DR GO; GO:0031573; P:mitotic intra-S DNA damage checkpoint signaling; IMP:PomBase.
DR GO; GO:0062110; P:negative regulation of DNA recombinase disassembly; IDA:PomBase.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:PomBase.
DR GO; GO:0042148; P:strand invasion; IDA:PomBase.
DR IDEAL; IID50104; -.
DR InterPro; IPR018468; SFR1/Mei5.
DR Pfam; PF10376; Mei5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chromosome partition; Coiled coil; Cytoplasm; DNA damage;
KW DNA repair; Meiosis; Nucleus; Reference proteome.
FT CHAIN 1..299
FT /note="Swi5-dependent recombination DNA repair protein 1"
FT /id="PRO_0000097710"
FT REGION 1..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 132..152
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 177..236
FT /evidence="ECO:0000255"
FT COMPBIAS 1..21
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..52
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 3
FT /note="Q -> H (in Ref. 3; BAA12180)"
FT /evidence="ECO:0000305"
FT CONFLICT 80
FT /note="K -> Q (in Ref. 3; BAA12180)"
FT /evidence="ECO:0000305"
FT CONFLICT 172
FT /note="N -> T (in Ref. 3; BAA12180)"
FT /evidence="ECO:0000305"
FT CONFLICT 209..214
FT /note="VEAKNE -> GGTKNG (in Ref. 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 216..299
FT /note="Missing (in Ref. 3)"
FT /evidence="ECO:0000305"
FT HELIX 181..212
FT /evidence="ECO:0007829|PDB:3VIQ"
FT HELIX 215..245
FT /evidence="ECO:0007829|PDB:3VIQ"
FT TURN 246..248
FT /evidence="ECO:0007829|PDB:3VIQ"
FT STRAND 250..255
FT /evidence="ECO:0007829|PDB:3VIQ"
FT TURN 260..263
FT /evidence="ECO:0007829|PDB:3VIQ"
FT STRAND 265..270
FT /evidence="ECO:0007829|PDB:3VIQ"
FT HELIX 274..280
FT /evidence="ECO:0007829|PDB:3VIQ"
FT TURN 285..287
FT /evidence="ECO:0007829|PDB:3VIQ"
FT TURN 292..295
FT /evidence="ECO:0007829|PDB:3VIQ"
SQ SEQUENCE 299 AA; 33638 MW; EFE91ABDE965C82D CRC64;
MSQTINSELN ENATSQCKED LKVSLSESDL RDSQGQLGIE NPPKCNNSGN HSDNLGFIEQ
SETVHPENEK ALTPDLRDTK IHTSLPITTP FSKKRAREAK NILLKPFKSP LRQTASPQVA
DTNLKPSLAV TNLNSDETNT SSEPVTSPLR TTPNSIKRQK RLFKSPISNC LNPKSDPEIT
QLLSRRLKLE KEVRNLQEQL ITAETARKVE AKNEDKDLQT LIQKWKNAAQ QAAEVLFKPM
AERIRLAGGV TQSFRIEEGE NKGQIQEVRT EFTMSMFLNQ FGVPVHLMSF DEENGDWKS
