SFRP1_RAT
ID SFRP1_RAT Reviewed; 158 AA.
AC Q9R168;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Secreted frizzled-related protein 1;
DE Short=sFRP-1;
DE Flags: Fragment;
GN Name=Sfrp1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=Fischer;
RX PubMed=11287180; DOI=10.1016/s0925-4773(01)00282-9;
RA Yoshino K., Rubin J.S., Higinbotham K.G., Uren A., Anest V., Plisov S.Y.,
RA Perantoni A.O.;
RT "Secreted Frizzled-related proteins can regulate metanephric development.";
RL Mech. Dev. 102:45-55(2001).
CC -!- FUNCTION: Soluble frizzled-related proteins (sFRPS) function as
CC modulators of Wnt signaling through direct interaction with Wnts. They
CC have a role in regulating cell growth and differentiation in specific
CC cell types. SFRP1 decreases intracellular beta-catenin levels (By
CC similarity). Has antiproliferative effects on vascular cells, in vitro
CC and in vivo, and can induce, in vivo, an angiogenic response. In
CC vascular cell cycle, delays the G1 phase and entry into the S phase. In
CC kidney development, inhibits tubule formation and bud growth in
CC metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with WNT4, WNT1, WNT2, WNT8, MYOC and FRZD6.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Note=Cell membrane or
CC extracellular matrix-associated. Released by heparin-binding (By
CC similarity). {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Differentially expressed in developing kidney. In
CC 16 dpc embryos, highly expressed around the ureter and, also in all
CC stromal elements, including the interstitial populations of the medulla
CC and the outer layer of the cortex. In 19 dpc embryos, expression in the
CC medulla is increased and is also found in the mesenchymal tissue
CC surrounding the developing ureter. {ECO:0000269|PubMed:11287180}.
CC -!- DOMAIN: The FZ domain is involved in binding with Wnt ligands.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the secreted frizzled-related protein (sFRP)
CC family. {ECO:0000305}.
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DR EMBL; AF167308; AAD49337.1; -; mRNA.
DR AlphaFoldDB; Q9R168; -.
DR SMR; Q9R168; -.
DR IntAct; Q9R168; 1.
DR STRING; 10116.ENSRNOP00000024128; -.
DR ChEMBL; CHEMBL1075241; -.
DR GlyGen; Q9R168; 1 site.
DR PaxDb; Q9R168; -.
DR PRIDE; Q9R168; -.
DR UCSC; RGD:621074; rat.
DR RGD; 621074; Sfrp1.
DR eggNOG; KOG3577; Eukaryota.
DR InParanoid; Q9R168; -.
DR PhylomeDB; Q9R168; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0009986; C:cell surface; ISO:RGD.
DR GO; GO:0062023; C:collagen-containing extracellular matrix; ISO:RGD.
DR GO; GO:0005829; C:cytosol; ISO:RGD.
DR GO; GO:0005615; C:extracellular space; ISO:RGD.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; ISO:RGD.
DR GO; GO:0005109; F:frizzled binding; ISO:RGD.
DR GO; GO:0008201; F:heparin binding; ISO:RGD.
DR GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR GO; GO:0017147; F:Wnt-protein binding; ISO:RGD.
DR GO; GO:0009952; P:anterior/posterior pattern specification; ISO:RGD.
DR GO; GO:0060346; P:bone trabecula formation; ISO:RGD.
DR GO; GO:0060070; P:canonical Wnt signaling pathway; ISO:RGD.
DR GO; GO:0071773; P:cellular response to BMP stimulus; ISO:RGD.
DR GO; GO:0071392; P:cellular response to estradiol stimulus; ISO:RGD.
DR GO; GO:0071391; P:cellular response to estrogen stimulus; ISO:RGD.
DR GO; GO:0044344; P:cellular response to fibroblast growth factor stimulus; ISO:RGD.
DR GO; GO:0071363; P:cellular response to growth factor stimulus; ISO:RGD.
DR GO; GO:0071504; P:cellular response to heparin; ISO:RGD.
DR GO; GO:0071456; P:cellular response to hypoxia; ISO:RGD.
DR GO; GO:0071347; P:cellular response to interleukin-1; ISO:RGD.
DR GO; GO:0071380; P:cellular response to prostaglandin E stimulus; IEP:RGD.
DR GO; GO:0009267; P:cellular response to starvation; ISO:RGD.
DR GO; GO:0071560; P:cellular response to transforming growth factor beta stimulus; ISO:RGD.
DR GO; GO:0071356; P:cellular response to tumor necrosis factor; ISO:RGD.
DR GO; GO:0071305; P:cellular response to vitamin D; ISO:RGD.
DR GO; GO:0071481; P:cellular response to X-ray; ISO:RGD.
DR GO; GO:0090246; P:convergent extension involved in somitogenesis; ISO:RGD.
DR GO; GO:0046546; P:development of primary male sexual characteristics; ISO:RGD.
DR GO; GO:0048546; P:digestive tract morphogenesis; ISO:RGD.
DR GO; GO:0071542; P:dopaminergic neuron differentiation; ISO:RGD.
DR GO; GO:0009950; P:dorsal/ventral axis specification; ISO:RGD.
DR GO; GO:0008585; P:female gonad development; ISO:RGD.
DR GO; GO:0002244; P:hematopoietic progenitor cell differentiation; ISO:RGD.
DR GO; GO:0060218; P:hematopoietic stem cell differentiation; ISO:RGD.
DR GO; GO:0030097; P:hemopoiesis; ISO:RGD.
DR GO; GO:0008584; P:male gonad development; ISO:RGD.
DR GO; GO:0060766; P:negative regulation of androgen receptor signaling pathway; ISO:RGD.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:RGD.
DR GO; GO:0045578; P:negative regulation of B cell differentiation; ISO:RGD.
DR GO; GO:0030514; P:negative regulation of BMP signaling pathway; ISO:RGD.
DR GO; GO:0046851; P:negative regulation of bone remodeling; ISO:RGD.
DR GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; IDA:UniProtKB.
DR GO; GO:2000080; P:negative regulation of canonical Wnt signaling pathway involved in controlling type B pancreatic cell proliferation; ISO:RGD.
DR GO; GO:0030308; P:negative regulation of cell growth; ISO:RGD.
DR GO; GO:0030336; P:negative regulation of cell migration; ISO:RGD.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISO:RGD.
DR GO; GO:0050680; P:negative regulation of epithelial cell proliferation; ISO:RGD.
DR GO; GO:0010719; P:negative regulation of epithelial to mesenchymal transition; ISO:RGD.
DR GO; GO:2000270; P:negative regulation of fibroblast apoptotic process; ISO:RGD.
DR GO; GO:0048147; P:negative regulation of fibroblast proliferation; ISO:RGD.
DR GO; GO:0010629; P:negative regulation of gene expression; ISO:RGD.
DR GO; GO:0046676; P:negative regulation of insulin secretion; ISO:RGD.
DR GO; GO:0043508; P:negative regulation of JUN kinase activity; ISO:RGD.
DR GO; GO:0030279; P:negative regulation of ossification; ISO:RGD.
DR GO; GO:0045668; P:negative regulation of osteoblast differentiation; ISO:RGD.
DR GO; GO:0033689; P:negative regulation of osteoblast proliferation; ISO:RGD.
DR GO; GO:0045671; P:negative regulation of osteoclast differentiation; IMP:RGD.
DR GO; GO:0050732; P:negative regulation of peptidyl-tyrosine phosphorylation; ISO:RGD.
DR GO; GO:2000041; P:negative regulation of planar cell polarity pathway involved in axis elongation; ISO:RGD.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0030178; P:negative regulation of Wnt signaling pathway; IDA:RGD.
DR GO; GO:2000054; P:negative regulation of Wnt signaling pathway involved in dorsal/ventral axis specification; ISO:RGD.
DR GO; GO:0014034; P:neural crest cell fate commitment; ISO:RGD.
DR GO; GO:0001843; P:neural tube closure; ISO:RGD.
DR GO; GO:0021915; P:neural tube development; ISO:RGD.
DR GO; GO:0001649; P:osteoblast differentiation; IEP:UniProtKB.
DR GO; GO:0090179; P:planar cell polarity pathway involved in neural tube closure; ISO:RGD.
DR GO; GO:0043065; P:positive regulation of apoptotic process; ISO:RGD.
DR GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; ISO:RGD.
DR GO; GO:0030307; P:positive regulation of cell growth; ISO:RGD.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:RGD.
DR GO; GO:0050679; P:positive regulation of epithelial cell proliferation; ISO:RGD.
DR GO; GO:2001238; P:positive regulation of extrinsic apoptotic signaling pathway; ISO:RGD.
DR GO; GO:1902043; P:positive regulation of extrinsic apoptotic signaling pathway via death domain receptors; ISO:RGD.
DR GO; GO:0045600; P:positive regulation of fat cell differentiation; ISO:RGD.
DR GO; GO:2000271; P:positive regulation of fibroblast apoptotic process; ISO:RGD.
DR GO; GO:2000052; P:positive regulation of non-canonical Wnt signaling pathway; ISO:RGD.
DR GO; GO:0045880; P:positive regulation of smoothened signaling pathway; ISO:RGD.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0030177; P:positive regulation of Wnt signaling pathway; ISO:RGD.
DR GO; GO:0060527; P:prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis; ISO:RGD.
DR GO; GO:0060687; P:regulation of branching involved in prostate gland morphogenesis; ISO:RGD.
DR GO; GO:0010564; P:regulation of cell cycle process; ISO:RGD.
DR GO; GO:0090175; P:regulation of establishment of planar polarity; ISO:RGD.
DR GO; GO:1904956; P:regulation of midbrain dopaminergic neuron differentiation; ISO:RGD.
DR GO; GO:0010975; P:regulation of neuron projection development; ISO:RGD.
DR GO; GO:0030278; P:regulation of ossification; IMP:RGD.
DR GO; GO:0014070; P:response to organic cyclic compound; ISO:RGD.
DR GO; GO:0009410; P:response to xenobiotic stimulus; ISO:RGD.
DR GO; GO:0035019; P:somatic stem cell population maintenance; ISO:RGD.
DR GO; GO:0001756; P:somitogenesis; ISO:RGD.
DR GO; GO:0044345; P:stromal-epithelial cell signaling involved in prostate gland development; ISO:RGD.
DR GO; GO:0001657; P:ureteric bud development; ISO:RGD.
DR GO; GO:0090244; P:Wnt signaling pathway involved in somitogenesis; ISO:RGD.
DR GO; GO:0060071; P:Wnt signaling pathway, planar cell polarity pathway; ISO:RGD.
DR Gene3D; 1.10.2000.10; -; 1.
DR Gene3D; 2.40.50.120; -; 1.
DR InterPro; IPR015526; Frizzled/SFRP.
DR InterPro; IPR020067; Frizzled_dom.
DR InterPro; IPR036790; Frizzled_dom_sf.
DR InterPro; IPR001134; Netrin_domain.
DR InterPro; IPR018933; Netrin_module_non-TIMP.
DR InterPro; IPR026559; SFRP1.
DR InterPro; IPR008993; TIMP-like_OB-fold.
DR PANTHER; PTHR11309; PTHR11309; 1.
DR PANTHER; PTHR11309:SF87; PTHR11309:SF87; 1.
DR Pfam; PF01759; NTR; 1.
DR SMART; SM00643; C345C; 1.
DR SUPFAM; SSF50242; SSF50242; 1.
DR SUPFAM; SSF63501; SSF63501; 1.
DR PROSITE; PS50038; FZ; 1.
DR PROSITE; PS50189; NTR; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; Disulfide bond; Glycoprotein;
KW Reference proteome; Secreted; Wnt signaling pathway.
FT CHAIN <1..>158
FT /note="Secreted frizzled-related protein 1"
FT /id="PRO_0000191647"
FT DOMAIN <1..34
FT /note="FZ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00090"
FT DOMAIN 51..>158
FT /note="NTR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00295"
FT CARBOHYD 38
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
FT DISULFID 51..121
FT /evidence="ECO:0000250"
FT DISULFID 68..123
FT /evidence="ECO:0000250"
FT NON_TER 1
FT NON_TER 158
SQ SEQUENCE 158 AA; 18072 MW; ABB1F13E3FB62690 CRC64;
VRDSCEPVMQ FFGFYWPEML KCDKFPEGDV CIAMTPPNAT EASKPQGTTV CPPCDNELKS
EAIIEHLCAS EFALRMKIKE VKKENGDKKI VPKKKKPLKL GPIKKKELKR LVLFLKNGAD
CPCHQLDNLS HNFLIMGRKV KSQYLLTAIH KWDKKNKE
