ABEC2_BOVIN
ID ABEC2_BOVIN Reviewed; 224 AA.
AC Q3SYR3;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Probable C->U-editing enzyme APOBEC-2;
DE EC=3.5.4.36;
DE AltName: Full=mRNA(cytosine(6666)) deaminase 2;
GN Name=APOBEC2;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Korean;
RA Chung H.J., Jung K.C., Lee Y.J., Lee J.H., Yoon D.H., Lee S.H., Sang B.C.;
RT "Characterization of apolipoprotein B mRNA editing enzyme catalytic
RT polypeptide-like 2 (APOBEC-2) in Korean cattle.";
RL Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Heart ventricle;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probable C to U editing enzyme whose physiological substrate
CC is not yet known. Does not display detectable apoB mRNA editing. Has a
CC low intrinsic cytidine deaminase activity. May play a role in the
CC epigenetic regulation of gene expression through the process of active
CC DNA demethylation. {ECO:0000250|UniProtKB:Q9Y235}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cytidine(6666) in apoB mRNA + H(+) + H2O = NH4(+) +
CC uridine(6666) in apoB mRNA; Xref=Rhea:RHEA:21772, Rhea:RHEA-
CC COMP:13888, Rhea:RHEA-COMP:13889, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, ChEBI:CHEBI:65315,
CC ChEBI:CHEBI:82748; EC=3.5.4.36;
CC Evidence={ECO:0000250|UniProtKB:Q9Y235};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000250|UniProtKB:Q9Y235};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000250|UniProtKB:Q9Y235};
CC -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:Q9Y235}.
CC -!- SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase
CC family. {ECO:0000305}.
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DR EMBL; DQ280381; ABB90107.1; -; mRNA.
DR EMBL; BC103438; AAI03439.1; -; mRNA.
DR RefSeq; NP_001029527.1; NM_001034355.1.
DR AlphaFoldDB; Q3SYR3; -.
DR SMR; Q3SYR3; -.
DR STRING; 9913.ENSBTAP00000035907; -.
DR PaxDb; Q3SYR3; -.
DR PRIDE; Q3SYR3; -.
DR GeneID; 509619; -.
DR KEGG; bta:509619; -.
DR CTD; 10930; -.
DR eggNOG; ENOG502RABR; Eukaryota.
DR HOGENOM; CLU_080056_0_0_1; -.
DR InParanoid; Q3SYR3; -.
DR OrthoDB; 1380685at2759; -.
DR TreeFam; TF331356; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004126; F:cytidine deaminase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0016554; P:cytidine to uridine editing; IBA:GO_Central.
DR GO; GO:0080111; P:DNA demethylation; ISS:UniProtKB.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR InterPro; IPR002125; CMP_dCMP_dom.
DR InterPro; IPR016193; Cytidine_deaminase-like.
DR SUPFAM; SSF53927; SSF53927; 1.
DR PROSITE; PS51747; CYT_DCMP_DEAMINASES_2; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Metal-binding; mRNA processing; Reference proteome; Zinc.
FT CHAIN 1..224
FT /note="Probable C->U-editing enzyme APOBEC-2"
FT /id="PRO_0000244422"
FT DOMAIN 64..169
FT /note="CMP/dCMP-type deaminase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01083"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 100
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q9Y235"
FT BINDING 60
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250|UniProtKB:Q9Y235"
FT BINDING 98
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250|UniProtKB:Q9Y235"
FT BINDING 128
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250|UniProtKB:Q9Y235"
FT BINDING 131
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250|UniProtKB:Q9Y235"
SQ SEQUENCE 224 AA; 25962 MW; 54881038F34C504C CRC64;
MAQKEEAAAA AEPASQNGEE VENLEDPEKL KELIELPPFE IVTGERLPAH YFKFQFRNVE
YSSGRNKTFL CYVVEAQSKG GQVQASRGYL EDEHATNHAE EAFFNSIMPT FDPALRYMVT
WYVSSSPCAA CADRIVKTLN KTKNLRLLIL VGRLFMWEEP EIQAALRKLK EAGCRLRIMK
PQDFEYIWQN FVEQEEGESK AFEPWEDIQE NFLYYEEKLA DILK