SFT2B_HUMAN
ID SFT2B_HUMAN Reviewed; 160 AA.
AC O95562; A8K2R0; Q6UWR8;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Vesicle transport protein SFT2B;
DE AltName: Full=SFT2 domain-containing protein 2;
GN Name=SFT2D2 {ECO:0000312|EMBL:AAH68098.1}; ORFNames=UNQ512/PRO1027;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1] {ECO:0000312|EMBL:CAA22897.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Rhodes S.;
RL Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Tongue;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [4] {ECO:0000312|EMBL:CAA22897.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000312|EMBL:AAH68098.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lymph {ECO:0000312|EMBL:AAH68098.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6] {ECO:0000312|EMBL:AAQ89038.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 74-160.
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [10]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [11]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [12]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- FUNCTION: May be involved in fusion of retrograde transport vesicles
CC derived from an endocytic compartment with the Golgi complex.
CC {ECO:0000250|UniProtKB:P38166}.
CC -!- INTERACTION:
CC O95562; Q13520: AQP6; NbExp=3; IntAct=EBI-4402330, EBI-13059134;
CC O95562; P18859: ATP5PF; NbExp=3; IntAct=EBI-4402330, EBI-2606700;
CC O95562; Q12982: BNIP2; NbExp=3; IntAct=EBI-4402330, EBI-752094;
CC O95562; Q9GZR5: ELOVL4; NbExp=3; IntAct=EBI-4402330, EBI-18535450;
CC O95562; Q8TDT2: GPR152; NbExp=3; IntAct=EBI-4402330, EBI-13345167;
CC O95562; P31937: HIBADH; NbExp=3; IntAct=EBI-4402330, EBI-11427100;
CC O95562; Q8NBQ5: HSD17B11; NbExp=3; IntAct=EBI-4402330, EBI-1052304;
CC O95562; Q7Z5P4: HSD17B13; NbExp=3; IntAct=EBI-4402330, EBI-18053395;
CC O95562; P38484: IFNGR2; NbExp=3; IntAct=EBI-4402330, EBI-3905457;
CC O95562; Q9BT40: INPP5K; NbExp=3; IntAct=EBI-4402330, EBI-749162;
CC O95562; Q70IA6: MOB2; NbExp=3; IntAct=EBI-4402330, EBI-2558739;
CC O95562; Q9ULP0-2: NDRG4; NbExp=3; IntAct=EBI-4402330, EBI-11978907;
CC O95562; Q96AL5: PBX3; NbExp=3; IntAct=EBI-4402330, EBI-741171;
CC O95562; Q99541: PLIN2; NbExp=3; IntAct=EBI-4402330, EBI-2115275;
CC O95562; O60664: PLIN3; NbExp=3; IntAct=EBI-4402330, EBI-725795;
CC O95562; Q9BRK0: REEP2; NbExp=3; IntAct=EBI-4402330, EBI-11337973;
CC O95562; Q9H6H4: REEP4; NbExp=3; IntAct=EBI-4402330, EBI-7545592;
CC O95562; Q14973: SLC10A1; NbExp=3; IntAct=EBI-4402330, EBI-3923031;
CC O95562; Q3KNW5: SLC10A6; NbExp=3; IntAct=EBI-4402330, EBI-18159983;
CC O95562; Q8IWU4: SLC30A8; NbExp=3; IntAct=EBI-4402330, EBI-10262251;
CC O95562; P27105: STOM; NbExp=3; IntAct=EBI-4402330, EBI-1211440;
CC O95562; Q9NYW4: TAS2R5; NbExp=3; IntAct=EBI-4402330, EBI-17933167;
CC O95562; O43615: TIMM44; NbExp=3; IntAct=EBI-4402330, EBI-861737;
CC O95562; Q96AN5: TMEM143; NbExp=3; IntAct=EBI-4402330, EBI-13342951;
CC O95562; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-4402330, EBI-8638294;
CC O95562; Q9NW97: TMEM51; NbExp=3; IntAct=EBI-4402330, EBI-726044;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the SFT2 family. {ECO:0000255}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAQ89038.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AL035297; CAA22897.1; -; mRNA.
DR EMBL; AK290325; BAF83014.1; -; mRNA.
DR EMBL; AL009051; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471067; EAW90822.1; -; Genomic_DNA.
DR EMBL; BC068098; AAH68098.1; -; mRNA.
DR EMBL; AY358675; AAQ89038.1; ALT_INIT; mRNA.
DR CCDS; CCDS1271.1; -.
DR RefSeq; NP_955376.1; NM_199344.2.
DR AlphaFoldDB; O95562; -.
DR BioGRID; 131951; 39.
DR IntAct; O95562; 28.
DR STRING; 9606.ENSP00000271375; -.
DR iPTMnet; O95562; -.
DR PhosphoSitePlus; O95562; -.
DR SwissPalm; O95562; -.
DR BioMuta; SFT2D2; -.
DR EPD; O95562; -.
DR jPOST; O95562; -.
DR MassIVE; O95562; -.
DR MaxQB; O95562; -.
DR PaxDb; O95562; -.
DR PeptideAtlas; O95562; -.
DR PRIDE; O95562; -.
DR ProteomicsDB; 50941; -.
DR Antibodypedia; 50453; 164 antibodies from 22 providers.
DR DNASU; 375035; -.
DR Ensembl; ENST00000271375.7; ENSP00000271375.3; ENSG00000213064.10.
DR GeneID; 375035; -.
DR KEGG; hsa:375035; -.
DR MANE-Select; ENST00000271375.7; ENSP00000271375.3; NM_199344.3; NP_955376.1.
DR UCSC; uc001gfk.3; human.
DR CTD; 375035; -.
DR GeneCards; SFT2D2; -.
DR HGNC; HGNC:25140; SFT2D2.
DR HPA; ENSG00000213064; Low tissue specificity.
DR neXtProt; NX_O95562; -.
DR OpenTargets; ENSG00000213064; -.
DR PharmGKB; PA142670928; -.
DR VEuPathDB; HostDB:ENSG00000213064; -.
DR eggNOG; KOG2887; Eukaryota.
DR GeneTree; ENSGT00390000018525; -.
DR HOGENOM; CLU_099529_2_2_1; -.
DR InParanoid; O95562; -.
DR OMA; FWWRKAG; -.
DR OrthoDB; 1476123at2759; -.
DR PhylomeDB; O95562; -.
DR TreeFam; TF315157; -.
DR PathwayCommons; O95562; -.
DR SignaLink; O95562; -.
DR BioGRID-ORCS; 375035; 12 hits in 1077 CRISPR screens.
DR ChiTaRS; SFT2D2; human.
DR GenomeRNAi; 375035; -.
DR Pharos; O95562; Tdark.
DR PRO; PR:O95562; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; O95562; protein.
DR Bgee; ENSG00000213064; Expressed in sperm and 195 other tissues.
DR ExpressionAtlas; O95562; baseline and differential.
DR Genevisible; O95562; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:InterPro.
DR InterPro; IPR007305; Vesicle_transpt_Got1/SFT2.
DR InterPro; IPR011691; Vesicle_transpt_SFT2.
DR PANTHER; PTHR23137; PTHR23137; 1.
DR Pfam; PF04178; Got1; 1.
PE 1: Evidence at protein level;
KW Acetylation; Membrane; Phosphoprotein; Protein transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..160
FT /note="Vesicle transport protein SFT2B"
FT /id="PRO_0000238610"
FT TOPO_DOM 1..36
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 37..57
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 58..63
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..84
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 85..98
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..123
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..160
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT MOD_RES 9
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231,
FT ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163"
SQ SEQUENCE 160 AA; 17779 MW; F05C57532B7593BD CRC64;
MDKLKKVLSG QDTEDRSGLS EVVEASSLSW STRIKGFIAC FAIGILCSLL GTVLLWVPRK
GLHLFAVFYT FGNIASIGST IFLMGPVKQL KRMFEPTRLI ATIMVLLCFA LTLCSAFWWH
NKGLALIFCI LQSLALTWYS LSFIPFARDA VKKCFAVCLA
