BGLH_ECOLI
ID BGLH_ECOLI Reviewed; 538 AA.
AC P26218; P76743; Q2M838;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 3.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Cryptic outer membrane porin BglH;
DE Flags: Precursor;
GN Name=bglH; Synonyms=yieC; OrderedLocusNames=b3720, JW3698;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7686882; DOI=10.1006/geno.1993.1230;
RA Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.;
RT "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome:
RT organizational symmetry around the origin of replication.";
RL Genomics 16:551-561(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO
RP 202-251.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-113.
RC STRAIN=K12;
RX PubMed=3034860; DOI=10.1128/jb.169.6.2579-2590.1987;
RA Schnetz K., Toloczyki C., Rak B.;
RT "Beta-glucoside (bgl) operon of Escherichia coli K-12: nucleotide sequence,
RT genetic organization, and possible evolutionary relationship to regulatory
RT components of two Bacillus subtilis genes.";
RL J. Bacteriol. 169:2579-2590(1987).
RN [5]
RP OVEREXPRESSION OF 125-538.
RC STRAIN=K12;
RX PubMed=9426150;
RA Wang J., Betton J.-M., Michel V., Hofnung M., Charbit A.;
RT "Identification of a cryptic porin gene in the Escherichia coli genome:
RT expression and insertion of a monomeric form of the protein into the outer
RT membrane.";
RL Mol. Microbiol. 26:1141-1143(1997).
RN [6]
RP OVEREXPRESSION, CHARACTERIZATION, SUBCELLULAR LOCATION, AND SUBSTRATE
RP SPECIFICITY.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=10027967; DOI=10.1046/j.1365-2958.1999.01191.x;
RA Andersen C., Rak B., Benz R.;
RT "The gene bglH present in the bgl operon of Escherichia coli, responsible
RT for uptake and fermentation of beta-glucosides encodes for a carbohydrate-
RT specific outer membrane porin.";
RL Mol. Microbiol. 31:499-510(1999).
CC -!- FUNCTION: Part of a cryptic operon that is poorly expressed in vivo.
CC May be an ancestral sugar porin with a broad carbohydrate specificity;
CC it binds aromatic beta-D-glucosides such as arbutin and salicin, but
CC with low affinity compared to the binding of maltooligosaccharides to
CC the LamB porin.
CC -!- SUBUNIT: Homomonomer; no physical evidence of a homotrimer has been
CC found (PubMed:9426150, PubMed:10027967), however conductance
CC experiments (PubMed:10027967) suggest it may be a homotrimer. The
CC monomer probably consists of 18 antiparallel beta-strands.
CC {ECO:0000269|PubMed:10027967, ECO:0000269|PubMed:9426150}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000305|PubMed:10027967}; Multi-pass membrane protein
CC {ECO:0000305|PubMed:10027967}. Note=Can be easily removed from the
CC outer membrane by treatment with detergent.
CC -!- SIMILARITY: Belongs to the porin LamB (TC 1.B.3) family. {ECO:0000305}.
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DR EMBL; L10328; AAA62071.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76743.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77568.1; -; Genomic_DNA.
DR EMBL; M16487; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A65175; A65175.
DR RefSeq; NP_418176.1; NC_000913.3.
DR RefSeq; WP_000489881.1; NZ_LN832404.1.
DR AlphaFoldDB; P26218; -.
DR SMR; P26218; -.
DR BioGRID; 4261194; 167.
DR STRING; 511145.b3720; -.
DR TCDB; 1.B.3.1.3; the sugar porin (sp) family.
DR PaxDb; P26218; -.
DR PRIDE; P26218; -.
DR EnsemblBacteria; AAC76743; AAC76743; b3720.
DR EnsemblBacteria; BAE77568; BAE77568; BAE77568.
DR GeneID; 948228; -.
DR KEGG; ecj:JW3698; -.
DR KEGG; eco:b3720; -.
DR PATRIC; fig|1411691.4.peg.2981; -.
DR EchoBASE; EB1339; -.
DR eggNOG; COG4580; Bacteria.
DR HOGENOM; CLU_032473_2_1_6; -.
DR OMA; NTHTIDL; -.
DR PhylomeDB; P26218; -.
DR BioCyc; EcoCyc:EG11364-MON; -.
DR PRO; PR:P26218; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IDA:EcoCyc.
DR GO; GO:0045203; C:integral component of cell outer membrane; IBA:GO_Central.
DR GO; GO:0046930; C:pore complex; IBA:GO_Central.
DR GO; GO:0015144; F:carbohydrate transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0015288; F:porin activity; IBA:GO_Central.
DR GO; GO:0034219; P:carbohydrate transmembrane transport; IDA:EcoCyc.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0015774; P:polysaccharide transport; IBA:GO_Central.
DR CDD; cd01346; Maltoporin-like; 1.
DR Gene3D; 2.40.170.10; -; 1.
DR InterPro; IPR021570; LamB-type_porin_N_dom.
DR InterPro; IPR003192; Porin_LamB.
DR InterPro; IPR036998; Porin_LamB_sf.
DR Pfam; PF02264; LamB; 1.
DR Pfam; PF11471; Sugarporin_N; 1.
PE 1: Evidence at protein level;
KW Cell outer membrane; Ion transport; Membrane; Porin; Reference proteome;
KW Signal; Transmembrane; Transmembrane beta strand; Transport.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..538
FT /note="Cryptic outer membrane porin BglH"
FT /id="PRO_0000025188"
FT REGION 52..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 62..80
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 202..251
FT /note="GGENYMQFSDMYVTTKGFLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTD
FT -> VARTICSSPICTLPPKVSCPLAQRLISGWVNTVRRKLKSRCLTGKRSVLM (in
FT Ref. 1; AAA62071)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 538 AA; 60657 MW; C537613D6D39B8F9 CRC64;
MFRRNLITSA ILLMAPLAFS AQSLAESLTV EQRLELLEKA LRETQSELKK YKDEEKKKYT
PATVNRSVST NDQGYAANPF PTSSAAKPDA VLVKNEEKNA SETGSIYSSM TLKDFSKFVK
DEIGFSYNGY YRSGWGTASH GSPKSWAIGS LGRFGNEYSG WFDLQLKQRV YNENGKRVDA
VVMMDGNVGQ QYSTGWFGDN AGGENYMQFS DMYVTTKGFL PFAPEADFWV GKHGAPKIEI
QMLDWKTQRT DAAAGVGLEN WKVGPGKIDI ALVREDVDDY DRSLQNKQQI NTNTIDLRYK
DIPLWDKATL MVSGRYVTAN ESASEKDNQD NNGYYDWKDT WMFGTSLTQK FDKGGFNEFS
FLVANNSIAS NFGRYAGASP FTTFNGRYYG DHTGGTAVRL TSQGEAYIGD HFIVANAIVY
SFGNDIYSYE TGAHSDFESI RAVVRPAYIW DQYNQTGVEL GYFTQQNKDA NSNKFNESGY
KTTLFHTFKV NTSMLTSRPE IRFYATYIKA LENELDGFTF EDNKDDQFAV GAQAEIWW
