SFXN5_HUMAN
ID SFXN5_HUMAN Reviewed; 340 AA.
AC Q8TD22; A8K116; Q494Y3; Q53T29;
DT 09-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Sideroflexin-5 {ECO:0000303|PubMed:12039050};
GN Name=SFXN5 {ECO:0000303|PubMed:12039050, ECO:0000312|HGNC:HGNC:16073};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=12039050; DOI=10.1016/s0378-1119(02)00402-x;
RA Lockhart P.J., Holtom B., Lincoln S., Hussey J., Zimprich A., Gasser T.,
RA Wszolek Z.K., Hardy J., Farrer M.J.;
RT "The human sideroflexin 5 (SFXN5) gene: sequence, expression analysis and
RT exclusion as a candidate for PARK3.";
RL Gene 285:229-237(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [7]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=30442778; DOI=10.1126/science.aat9528;
RA Kory N., Wyant G.A., Prakash G., Uit de Bos J., Bottanelli F., Pacold M.E.,
RA Chan S.H., Lewis C.A., Wang T., Keys H.R., Guo Y.E., Sabatini D.M.;
RT "SFXN1 is a mitochondrial serine transporter required for one-carbon
RT metabolism.";
RL Science 362:0-0(2018).
CC -!- FUNCTION: Mitochondrial amino-acid transporter (By similarity). Does
CC not act as a serine transporter: not able to mediate transport of
CC serine into mitochondria (PubMed:30442778). Transports citrate (By
CC similarity). {ECO:0000250|UniProtKB:Q8CFD0,
CC ECO:0000250|UniProtKB:Q9H9B4, ECO:0000269|PubMed:30442778}.
CC -!- INTERACTION:
CC Q8TD22; Q9BQE5: APOL2; NbExp=3; IntAct=EBI-17274136, EBI-4290634;
CC Q8TD22; Q13520: AQP6; NbExp=3; IntAct=EBI-17274136, EBI-13059134;
CC Q8TD22; Q96BA8: CREB3L1; NbExp=3; IntAct=EBI-17274136, EBI-6942903;
CC Q8TD22; Q15125: EBP; NbExp=3; IntAct=EBI-17274136, EBI-3915253;
CC Q8TD22; Q9Y282: ERGIC3; NbExp=3; IntAct=EBI-17274136, EBI-781551;
CC Q8TD22; P27105: STOM; NbExp=3; IntAct=EBI-17274136, EBI-1211440;
CC Q8TD22; Q96Q45-2: TMEM237; NbExp=3; IntAct=EBI-17274136, EBI-10982110;
CC Q8TD22; Q8N661: TMEM86B; NbExp=3; IntAct=EBI-17274136, EBI-2548832;
CC Q8TD22; Q6ZT21: TMPPE; NbExp=3; IntAct=EBI-17274136, EBI-11724433;
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane
CC {ECO:0000269|PubMed:30442778}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Primarily expressed in the brain.
CC {ECO:0000269|PubMed:12039050}.
CC -!- SIMILARITY: Belongs to the sideroflexin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY044437; AAK95826.1; -; mRNA.
DR EMBL; AK289731; BAF82420.1; -; mRNA.
DR EMBL; AC012366; AAY14745.1; -; Genomic_DNA.
DR EMBL; CH471053; EAW99754.1; -; Genomic_DNA.
DR EMBL; BC101311; AAI01312.1; -; mRNA.
DR EMBL; BC101312; AAI01313.1; -; mRNA.
DR EMBL; BC101313; AAI01314.1; -; mRNA.
DR CCDS; CCDS1922.1; -.
DR RefSeq; NP_653180.1; NM_144579.2.
DR AlphaFoldDB; Q8TD22; -.
DR BioGRID; 125109; 49.
DR IntAct; Q8TD22; 32.
DR STRING; 9606.ENSP00000272433; -.
DR iPTMnet; Q8TD22; -.
DR PhosphoSitePlus; Q8TD22; -.
DR BioMuta; SFXN5; -.
DR DMDM; 30580506; -.
DR EPD; Q8TD22; -.
DR jPOST; Q8TD22; -.
DR MassIVE; Q8TD22; -.
DR MaxQB; Q8TD22; -.
DR PaxDb; Q8TD22; -.
DR PeptideAtlas; Q8TD22; -.
DR PRIDE; Q8TD22; -.
DR ProteomicsDB; 74221; -.
DR Antibodypedia; 16510; 61 antibodies from 15 providers.
DR DNASU; 94097; -.
DR Ensembl; ENST00000272433.7; ENSP00000272433.2; ENSG00000144040.13.
DR GeneID; 94097; -.
DR KEGG; hsa:94097; -.
DR MANE-Select; ENST00000272433.7; ENSP00000272433.2; NM_144579.3; NP_653180.1.
DR UCSC; uc002siq.3; human.
DR CTD; 94097; -.
DR DisGeNET; 94097; -.
DR GeneCards; SFXN5; -.
DR HGNC; HGNC:16073; SFXN5.
DR HPA; ENSG00000144040; Tissue enhanced (brain).
DR MIM; 615572; gene.
DR neXtProt; NX_Q8TD22; -.
DR OpenTargets; ENSG00000144040; -.
DR PharmGKB; PA38088; -.
DR VEuPathDB; HostDB:ENSG00000144040; -.
DR eggNOG; KOG3767; Eukaryota.
DR GeneTree; ENSGT01030000234641; -.
DR HOGENOM; CLU_039425_2_1_1; -.
DR InParanoid; Q8TD22; -.
DR OMA; WINQSYN; -.
DR OrthoDB; 881974at2759; -.
DR PhylomeDB; Q8TD22; -.
DR TreeFam; TF313205; -.
DR PathwayCommons; Q8TD22; -.
DR SignaLink; Q8TD22; -.
DR BioGRID-ORCS; 94097; 8 hits in 1079 CRISPR screens.
DR ChiTaRS; SFXN5; human.
DR GenomeRNAi; 94097; -.
DR Pharos; Q8TD22; Tdark.
DR PRO; PR:Q8TD22; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q8TD22; protein.
DR Bgee; ENSG00000144040; Expressed in amygdala and 153 other tissues.
DR ExpressionAtlas; Q8TD22; baseline and differential.
DR Genevisible; Q8TD22; HS.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0015137; F:citrate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR GO; GO:0015746; P:citrate transport; IBA:GO_Central.
DR GO; GO:1990542; P:mitochondrial transmembrane transport; IBA:GO_Central.
DR InterPro; IPR004686; Mtc.
DR PANTHER; PTHR11153; PTHR11153; 1.
DR Pfam; PF03820; SFXNs; 1.
DR TIGRFAMs; TIGR00798; mtc; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Membrane; Mitochondrion; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..340
FT /note="Sideroflexin-5"
FT /id="PRO_0000177043"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 287..307
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 340 AA; 37124 MW; AF4103C7DB3F2DAC CRC64;
MADTATTASA AAASAASASS DAPPFQLGKP RFQQTSFYGR FRHFLDIIDP RTLFVTERRL
REAVQLLEDY KHGTLRPGVT NEQLWSAQKI KQAILHPDTN EKIFMPFRMS GYIPFGTPIV
VGLLLPNQTL ASTVFWQWLN QSHNACVNYA NRNATKPSPA SKFIQGYLGA VISAVSIAVG
LNVLVQKANK FTPATRLLIQ RFVPFPAVAS ANICNVVLMR YGELEEGIDV LDSDGNLVGS
SKIAARHALL ETALTRVVLP MPILVLPPIV MSMLEKTALL QARPRLLLPV QSLVCLAAFG
LALPLAISLF PQMSEIETSQ LEPEIAQATS SRTVVYNKGL
