SFXN5_MOUSE
ID SFXN5_MOUSE Reviewed; 342 AA.
AC Q925N0; Q8BGG3;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 09-MAY-2003, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Sideroflexin-5 {ECO:0000303|PubMed:11274051};
GN Name=Sfxn5 {ECO:0000303|PubMed:11274051, ECO:0000312|MGI:MGI:2137681};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE OF 1-181, AND TISSUE SPECIFICITY.
RX PubMed=11274051; DOI=10.1101/gad.873001;
RA Fleming M.D., Campagna D.R., Haslett J.N., Trenor C.C. III, Andrews N.C.;
RT "A mutation in a mitochondrial transmembrane protein is responsible for the
RT pleiotropic hematological and skeletal phenotype of flexed-tail (f/f)
RT mice.";
RL Genes Dev. 15:652-657(2001).
RN [3]
RP PROTEIN SEQUENCE OF 54-60; 64-73; 94-104; 204-244 AND 249-278, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Liver, and Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Mitochondrial amino-acid transporter (By similarity). Does
CC not act as a serine transporter: not able to mediate transport of
CC serine into mitochondria (By similarity). Transports citrate (By
CC similarity). {ECO:0000250|UniProtKB:Q8CFD0,
CC ECO:0000250|UniProtKB:Q8TD22, ECO:0000250|UniProtKB:Q9H9B4}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane
CC {ECO:0000250|UniProtKB:Q8CFD0}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in liver and brain.
CC {ECO:0000269|PubMed:11274051}.
CC -!- SIMILARITY: Belongs to the sideroflexin family. {ECO:0000305}.
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DR EMBL; AK036285; BAC29372.1; -; mRNA.
DR EMBL; AK082073; BAC38403.1; -; mRNA.
DR EMBL; AF325264; AAK39432.1; -; Transcribed_RNA.
DR CCDS; CCDS20291.1; -.
DR RefSeq; NP_848754.1; NM_178639.4.
DR AlphaFoldDB; Q925N0; -.
DR BioGRID; 220501; 8.
DR IntAct; Q925N0; 2.
DR MINT; Q925N0; -.
DR STRING; 10090.ENSMUSP00000041872; -.
DR TCDB; 2.A.54.1.5; the sideroflexin (sfxn) family (formerly the mitochondrial tricarboxylate carrier (mtc) family.
DR PhosphoSitePlus; Q925N0; -.
DR SwissPalm; Q925N0; -.
DR jPOST; Q925N0; -.
DR MaxQB; Q925N0; -.
DR PaxDb; Q925N0; -.
DR PRIDE; Q925N0; -.
DR ProteomicsDB; 261202; -.
DR Antibodypedia; 16510; 61 antibodies from 15 providers.
DR DNASU; 94282; -.
DR Ensembl; ENSMUST00000045846; ENSMUSP00000041872; ENSMUSG00000033720.
DR GeneID; 94282; -.
DR KEGG; mmu:94282; -.
DR UCSC; uc009cpj.2; mouse.
DR CTD; 94097; -.
DR MGI; MGI:2137681; Sfxn5.
DR VEuPathDB; HostDB:ENSMUSG00000033720; -.
DR eggNOG; KOG3767; Eukaryota.
DR GeneTree; ENSGT01030000234641; -.
DR HOGENOM; CLU_039425_2_1_1; -.
DR InParanoid; Q925N0; -.
DR OMA; WINQSYN; -.
DR OrthoDB; 881974at2759; -.
DR PhylomeDB; Q925N0; -.
DR TreeFam; TF313205; -.
DR BioGRID-ORCS; 94282; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Sfxn5; mouse.
DR PRO; PR:Q925N0; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q925N0; protein.
DR Bgee; ENSMUSG00000033720; Expressed in granulocyte and 192 other tissues.
DR ExpressionAtlas; Q925N0; baseline and differential.
DR Genevisible; Q925N0; MM.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:0005743; C:mitochondrial inner membrane; HDA:MGI.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0015137; F:citrate transmembrane transporter activity; ISO:MGI.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR GO; GO:0015746; P:citrate transport; ISO:MGI.
DR GO; GO:1990542; P:mitochondrial transmembrane transport; IBA:GO_Central.
DR InterPro; IPR004686; Mtc.
DR PANTHER; PTHR11153; PTHR11153; 1.
DR Pfam; PF03820; SFXNs; 1.
DR TIGRFAMs; TIGR00798; mtc; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Direct protein sequencing; Membrane; Mitochondrion;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..342
FT /note="Sideroflexin-5"
FT /id="PRO_0000177044"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..23
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 342 AA; 37329 MW; 9BDB8261D5EF79D9 CRC64;
MADTATTASA ASAAASAPNA STDAPPFQLG KPRFQQTSFY GRFRHFLDII DPRTLFVTEK
RLREAVQLLE DYKHGTLRPG VTNEQLWSAQ KIKQAILHPD TNEKIFMPFR MSGYIPFGTP
IVVGLLLPNQ TLASTVFWQW LNQSHNACVN YANRNATKPS PASKFIQGYL GAVISAVSIA
VGLNVLVQKA NKFTPATRLL VQRFVPFPAV ASANICNVVL MRYGELEEGI DVLDADGNLV
GSSKIAARHA LLETALTRVV LPMPILVLPP IVMSMLEKTA LLQARPRLLL PVHSLVCLAA
FGLALPLAIS LFPQMSEIET SQLEPEIARA TSSRTVVYNK GL
