SG11B_HUMAN
ID SG11B_HUMAN Reviewed; 103 AA.
AC Q08648; E9PFH0; Q546A0; Q6ZYB2; Q9H4P8; Q9H4P9; Q9H4Q0; Q9H4Q1; Q9H4Q2;
AC Q9NRT3; Q9NRV4; Q9NRV5; Q9NRV6; Q9NRV7; Q9NRV8;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 18-APR-2006, sequence version 4.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Sperm-associated antigen 11B {ECO:0000312|HGNC:HGNC:14534};
DE AltName: Full=Human epididymis-specific protein 2 {ECO:0000303|PubMed:10698202};
DE Short=He2 {ECO:0000303|PubMed:10698202};
DE AltName: Full=Protein EP2 {ECO:0000303|PubMed:11259252};
DE AltName: Full=Sperm antigen HE2 {ECO:0000303|PubMed:8167223};
DE Flags: Precursor;
GN Name=SPAG11B {ECO:0000312|HGNC:HGNC:14534};
GN Synonyms=EP2 {ECO:0000303|PubMed:11259252},
GN HE2 {ECO:0000303|PubMed:8167223};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM EP2A), AND TISSUE SPECIFICITY.
RX PubMed=8167223; DOI=10.1095/biolreprod50.3.516;
RA Osterhoff C., Kirchhoff C., Krull N., Ivell R.;
RT "Molecular cloning and characterization of a novel human sperm antigen
RT (HE2) specifically expressed in the proximal epididymis.";
RL Biol. Reprod. 50:516-525(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS EP2A AND EP2D).
RX PubMed=10698202; DOI=10.1210/endo.141.3.7389;
RA Hamil K.G., Sivashanmugam P., Richardson R.T., Grossman G., Ruben S.M.,
RA Mohler J.L., Petrusz P., O'Rand M.G., French F.S., Hall S.H.;
RT "HE2 beta and HE2 gamma, new members of an epididymis-specific family of
RT androgen-regulated proteins in the human.";
RL Endocrinology 141:1245-1253(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS EP2A; EP2B; EP2C; EP2D AND
RP EP2E), AND VARIANT GLN-77.
RX PubMed=11259252; DOI=10.1095/biolreprod64.4.1072;
RA Frohlich O., Po C., Young L.G.;
RT "Organization of the human gene encoding the epididymis-specific EP2
RT protein variants and its relationship to defensin genes.";
RL Biol. Reprod. 64:1072-1079(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM EP2C).
RA von Horsten H.H., Derr P., Schaefer B., Kirchhoff C.;
RT "SPAG11/Isoform HE2C, an atypical anionic beta-defensin-like peptide of the
RT proximal human epididymis.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA Platzer M., Shimizu N., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
CC -!- FUNCTION: Has antimicrobial activity against E.coli (By similarity).
CC Plays a role in the defense response in the male reproductive tract,
CC contributing to sperm maturation, storage and protection (By
CC similarity). {ECO:0000250|UniProtKB:Q8VBV2}.
CC -!- INTERACTION:
CC Q08648-4; Q5W5X9-3: TTC23; NbExp=3; IntAct=EBI-14835966, EBI-9090990;
CC Q08648-4; Q9UHD9: UBQLN2; NbExp=3; IntAct=EBI-14835966, EBI-947187;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=6;
CC Comment=Exon 6 is read in two different reading frames, one resulting
CC in the N-terminal amino acid sequences of EP2A, EP2B and EP2F and the
CC other in the N-terminal sequences of EP2D, EP2E and EP2G.;
CC Name=EP2A; Synonyms=HE2 alpha1;
CC IsoId=Q08648-1; Sequence=Displayed;
CC Name=EP2B;
CC IsoId=Q08648-2; Sequence=VSP_006208;
CC Name=EP2C; Synonyms=HE2C;
CC IsoId=Q08648-3; Sequence=VSP_006209;
CC Name=EP2D; Synonyms=HE2 beta1;
CC IsoId=Q08648-4; Sequence=VSP_006210;
CC Name=EP2E;
CC IsoId=Q08648-5; Sequence=VSP_044926, VSP_044927;
CC Name=EP2F;
CC IsoId=Q08648-6; Sequence=Not described;
CC -!- TISSUE SPECIFICITY: Specifically expressed in caput and proximal corpus
CC of epididymis (at protein level) (PubMed:8167223). Present in the
CC epididymal epithelium and on the sperm surface, with a subacrosomal
CC equatorial distribution on the sperm head (at protein level)
CC (PubMed:8167223). {ECO:0000269|PubMed:8167223}.
CC -!- SIMILARITY: Belongs to the SPAG11 family. {ECO:0000305}.
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DR EMBL; X67697; CAA47927.1; -; mRNA.
DR EMBL; AF168616; AAF37186.1; -; mRNA.
DR EMBL; AF168617; AAF37187.1; -; mRNA.
DR EMBL; AY005129; AAG21878.1; -; Genomic_DNA.
DR EMBL; AY005129; AAG21879.1; -; Genomic_DNA.
DR EMBL; AY005129; AAG21880.1; -; Genomic_DNA.
DR EMBL; AY005129; AAG21881.1; -; Genomic_DNA.
DR EMBL; AY005129; AAG21882.1; -; Genomic_DNA.
DR EMBL; AY005129; AAG21883.1; -; Genomic_DNA.
DR EMBL; AJ628017; CAF31328.1; -; mRNA.
DR EMBL; AJ635328; CAG25732.1; -; mRNA.
DR EMBL; AC130360; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS47774.1; -. [Q08648-4]
DR CCDS; CCDS5966.1; -. [Q08648-1]
DR CCDS; CCDS5967.1; -. [Q08648-3]
DR PIR; I37454; I37454.
DR RefSeq; NP_057596.1; NM_016512.3. [Q08648-1]
DR RefSeq; NP_478107.2; NM_058200.2.
DR RefSeq; NP_478108.2; NM_058201.2. [Q08648-4]
DR RefSeq; NP_478109.1; NM_058202.2.
DR RefSeq; NP_478110.1; NM_058203.2. [Q08648-3]
DR RefSeq; NP_478113.2; NM_058206.3. [Q08648-2]
DR RefSeq; NP_478114.2; NM_058207.2. [Q08648-5]
DR RefSeq; XP_005272449.2; XM_005272392.4. [Q08648-3]
DR AlphaFoldDB; Q08648; -.
DR BioGRID; 115678; 3.
DR IntAct; Q08648; 3.
DR TCDB; 1.C.85.1.5; the pore-forming Beta-defensin (Beta-defensin) family.
DR GlyGen; Q08648; 1 site.
DR BioMuta; SPAG11B; -.
DR DMDM; 93141314; -.
DR MassIVE; Q08648; -.
DR PeptideAtlas; Q08648; -.
DR PRIDE; Q08648; -.
DR ProteomicsDB; 20100; -.
DR ProteomicsDB; 58638; -. [Q08648-1]
DR ProteomicsDB; 58639; -. [Q08648-2]
DR ProteomicsDB; 58641; -. [Q08648-4]
DR TopDownProteomics; Q08648-2; -. [Q08648-2]
DR Antibodypedia; 22021; 49 antibodies from 12 providers.
DR DNASU; 10407; -.
DR Ensembl; ENST00000297498.6; ENSP00000297498.2; ENSG00000164871.18. [Q08648-1]
DR Ensembl; ENST00000317900.7; ENSP00000322591.5; ENSG00000164871.18. [Q08648-3]
DR Ensembl; ENST00000398462.7; ENSP00000381480.2; ENSG00000164871.18. [Q08648-4]
DR Ensembl; ENST00000458665.5; ENSP00000398550.1; ENSG00000164871.18. [Q08648-5]
DR Ensembl; ENST00000528168.3; ENSP00000431230.1; ENSG00000164871.18. [Q08648-2]
DR Ensembl; ENST00000619134.4; ENSP00000483205.1; ENSG00000274405.4. [Q08648-5]
DR Ensembl; ENST00000622314.3; ENSP00000481630.1; ENSG00000274405.4. [Q08648-2]
DR GeneID; 10407; -.
DR GeneID; 653423; -.
DR KEGG; hsa:10407; -.
DR MANE-Select; ENST00000398462.7; ENSP00000381480.2; NM_058201.4; NP_478108.2. [Q08648-4]
DR UCSC; uc003wri.4; human. [Q08648-1]
DR CTD; 10407; -.
DR CTD; 653423; -.
DR DisGeNET; 10407; -.
DR DisGeNET; 653423; -.
DR GeneCards; SPAG11B; -.
DR HGNC; HGNC:14534; SPAG11B.
DR HPA; ENSG00000164871; Tissue enriched (epididymis).
DR MIM; 606560; gene.
DR neXtProt; NX_Q08648; -.
DR OpenTargets; ENSG00000178287; -.
DR PharmGKB; PA37894; -.
DR VEuPathDB; HostDB:ENSG00000164871; -.
DR GeneTree; ENSGT00940000161432; -.
DR HOGENOM; CLU_178650_0_0_1; -.
DR InParanoid; Q08648; -.
DR OMA; ACCLHQN; -.
DR OrthoDB; 1204101at2759; -.
DR PhylomeDB; Q08648; -.
DR PathwayCommons; Q08648; -.
DR SignaLink; Q08648; -.
DR BioGRID-ORCS; 10407; 15 hits in 975 CRISPR screens.
DR ChiTaRS; SPAG11B; human.
DR GeneWiki; SPAG11B; -.
DR GenomeRNAi; 10407; -.
DR Pharos; Q08648; Tbio.
DR PRO; PR:Q08648; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q08648; protein.
DR Bgee; ENSG00000164871; Expressed in monocyte and 33 other tissues.
DR ExpressionAtlas; Q08648; baseline and differential.
DR Genevisible; Q08648; HS.
DR GO; GO:0005576; C:extracellular region; NAS:UniProtKB.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IMP:UniProtKB.
DR GO; GO:0051838; P:cytolysis by host of symbiont cells; IMP:UniProtKB.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0007283; P:spermatogenesis; TAS:ProtInc.
DR InterPro; IPR007988; Sperm_Ag_11A_B.
DR PANTHER; PTHR14081; PTHR14081; 1.
DR Pfam; PF05324; Sperm_Ag_HE2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Antibiotic; Antimicrobial; Defensin; Glycoprotein;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..103
FT /note="Sperm-associated antigen 11B"
FT /id="PRO_0000033185"
FT CARBOHYD 29
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..80
FT /note="MRQRLLPSVTSLLLVALLFPGSSQARHVNHSATEALGELRERAPGQGTNGFQ
FT LLRHAVKRDLLPPRTPPYQVHISHREAR -> MKVFFLFAVLFCLVQTNSGDVPPGIRN
FT TICHMQQGICRLFFCHSGEKKRDICSDPWNRCCVSNTDEEGKEKPEMDGRSGI (in
FT isoform EP2E)"
FT /evidence="ECO:0000305"
FT /id="VSP_044926"
FT VAR_SEQ 1..71
FT /note="MRQRLLPSVTSLLLVALLFPGSSQARHVNHSATEALGELRERAPGQGTNGFQ
FT LLRHAVKRDLLPPRTPPYQ -> MKVFFLFAVLFCLVQTNS (in isoform
FT EP2B)"
FT /evidence="ECO:0000305"
FT /id="VSP_006208"
FT VAR_SEQ 72..103
FT /note="VHISHREARGPSFRICVDFLGPRWARGCSTGN -> EPASDLKVVDCRRSEG
FT FCQEYCNYMETQVGYCSKKKDACCLH (in isoform EP2C)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_006209"
FT VAR_SEQ 72..103
FT /note="VHISHREARGPSFRICVDFLGPRWARGCSTGN -> GDVPPGIRNTICHMQQ
FT GICRLFFCHSGEKKRDICSDPWNRCCVSNTDEEGKEKPEMDGRSGI (in isoform
FT EP2D)"
FT /evidence="ECO:0000303|PubMed:10698202"
FT /id="VSP_006210"
FT VAR_SEQ 81..103
FT /note="Missing (in isoform EP2E)"
FT /evidence="ECO:0000305"
FT /id="VSP_044927"
FT VARIANT 77
FT /note="R -> Q (in dbSNP:rs2853658)"
FT /evidence="ECO:0000269|PubMed:11259252"
FT /id="VAR_005269"
FT VARIANT 89
FT /note="D -> G (in dbSNP:rs2738035)"
FT /id="VAR_053683"
FT CONFLICT Q08648-3:18
FT /note="L -> C (in Ref. 4; CAG25732)"
FT /evidence="ECO:0000305"
FT CONFLICT Q08648-4:84
FT /note="H -> R (in Ref. 2; AAF37187)"
FT /evidence="ECO:0000305"
FT CONFLICT Q08648-5:31
FT /note="H -> R (in Ref. 3; AAG21882)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 103 AA; 11431 MW; ABDD1CE4BB54ABD3 CRC64;
MRQRLLPSVT SLLLVALLFP GSSQARHVNH SATEALGELR ERAPGQGTNG FQLLRHAVKR
DLLPPRTPPY QVHISHREAR GPSFRICVDF LGPRWARGCS TGN
