SGCB_ECOLI
ID SGCB_ECOLI Reviewed; 92 AA.
AC P58035; Q2EEU3; Q2M610;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 27-APR-2001, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Putative phosphotransferase enzyme IIB component SgcB;
DE EC=2.7.1.-;
DE AltName: Full=Putative PTS system EIIB component;
GN Name=sgcB; OrderedLocusNames=b4565, JW5967;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP DISCUSSION OF SEQUENCE.
RA Reizer J., Charbit A., Reizer A., Saier M.H. Jr.;
RT "Novel phosphotransferases system genes revealed by bacterial genome
RT analysis: operons encoding homologues of sugar-specific permease domains of
RT the phosphotransferase system and pentose catabolic enzymes.";
RL Genome Sci. Technol. 1:53-75(1996).
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC system (sugar PTS), a major carbohydrate active -transport system,
CC catalyzes the phosphorylation of incoming sugar substrates
CC concomitantly with their translocation across the cell membrane.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a
CC cysteinyl or histidyl residue, depending on the transported sugar.
CC Then, it transfers the phosphoryl group to the sugar substrate
CC concomitantly with the sugar uptake processed by the EIIC domain.
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DR EMBL; U00096; ABD18717.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78296.1; -; Genomic_DNA.
DR RefSeq; WP_000722973.1; NZ_SSUV01000012.1.
DR RefSeq; YP_588476.1; NC_000913.3.
DR AlphaFoldDB; P58035; -.
DR SMR; P58035; -.
DR BioGRID; 4262742; 7.
DR ComplexPortal; CPX-5990; sgcABC sugar permease enzyme II complex.
DR STRING; 511145.b4565; -.
DR TCDB; 4.A.5.1.3; the pts galactitol (gat) family.
DR PaxDb; P58035; -.
DR PRIDE; P58035; -.
DR EnsemblBacteria; ABD18717; ABD18717; b4565.
DR EnsemblBacteria; BAE78296; BAE78296; BAE78296.
DR GeneID; 1450295; -.
DR KEGG; ecj:JW5967; -.
DR KEGG; eco:b4565; -.
DR PATRIC; fig|1411691.4.peg.2392; -.
DR eggNOG; COG3414; Bacteria.
DR HOGENOM; CLU_159248_3_3_6; -.
DR OMA; QVSMDQT; -.
DR PhylomeDB; P58035; -.
DR BioCyc; EcoCyc:MON0-2121; -.
DR BioCyc; MetaCyc:MON0-2121; -.
DR PRO; PR:P58035; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:1902495; C:transmembrane transporter complex; IC:ComplexPortal.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR GO; GO:0008643; P:carbohydrate transport; IC:ComplexPortal.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; ISM:EcoCyc.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0015795; P:sorbitol transmembrane transport; IC:ComplexPortal.
DR InterPro; IPR036095; PTS_EIIB-like_sf.
DR InterPro; IPR013011; PTS_EIIB_2.
DR InterPro; IPR003501; PTS_EIIB_2/3.
DR Pfam; PF02302; PTS_IIB; 1.
DR SUPFAM; SSF52794; SSF52794; 1.
DR PROSITE; PS51099; PTS_EIIB_TYPE_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Kinase; Phosphotransferase system; Reference proteome;
KW Sugar transport; Transferase; Transport.
FT CHAIN 1..92
FT /note="Putative phosphotransferase enzyme IIB component
FT SgcB"
FT /id="PRO_0000186690"
FT DOMAIN 1..92
FT /note="PTS EIIB type-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00422"
FT ACT_SITE 8
FT /note="Phosphocysteine intermediate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 92 AA; 9803 MW; 371BC568AF1F0862 CRC64;
MKKILVACGT GMSTSTMIAH KLQEFLTEQG ISATTAQCCL NEIPLNCNGM DLIVTSMRTN
SDYGIPTLNG AALLTGINDD ALKQQIKALL TQ
