SGE1_YEAST
ID SGE1_YEAST Reviewed; 543 AA.
AC P33335; D6W4J6; Q05720;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Protein SGE1;
DE AltName: Full=10-N-nonyl acridine orange resistance protein;
DE AltName: Full=Crystal violet resistance protein;
GN Name=SGE1; Synonyms=NOR1; OrderedLocusNames=YPR198W; ORFNames=P9677.3;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8349103; DOI=10.1093/genetics/134.3.675;
RA Amakasu H., Suzuki Y., Nishizawa M., Fukasawa T.;
RT "Isolation and characterization of SGE1: a yeast gene that partially
RT suppresses the gal11 mutation in multiple copies.";
RL Genetics 134:675-683(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8058039; DOI=10.1007/bf00285456;
RA Ehrenhofer-Murray A.E., Wuergler F.E., Sengstag C.;
RT "The Saccharomyces cerevisiae SGE1 gene product: a novel drug-resistance
RT protein within the major facilitator superfamily.";
RL Mol. Gen. Genet. 244:287-294(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 28383 / FL100 / VTT C-80102;
RA Urdaci M.C., Jacquot C.;
RT "Membrane protein confering resistance to drug.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9271105;
RX DOI=10.1002/(sici)1097-0061(199708)13:10<891::aid-yea138>3.0.co;2-u;
RA Jacquot C., Julien R., Guilloton M.;
RT "The Saccharomyces cerevisiae MFS superfamily SGE1 gene confers resistance
RT to cationic dyes.";
RL Yeast 13:891-902(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [7]
RP CHARACTERIZATION.
RX PubMed=9483795;
RX DOI=10.1002/(sici)1097-0061(19980115)14:1<49::aid-yea199>3.0.co;2-t;
RA Ehrenhofer-Murray A.E., Keller Seitz M.U., Sengstag C.;
RT "The Sge1 protein of Saccharomyces cerevisiae is a membrane-associated
RT multidrug transporter.";
RL Yeast 14:49-65(1998).
RN [8]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Drug export permease. Multi-copy suppressor of loss-of-
CC function mutation of GAL11. Involved specifically in transcription of
CC GAL4-dependent genes. Can link GAL4 with the basal transcription
CC machinery if GAL11 is missing. Confers resistance to 10-N-nonyl
CC acridine orange (NAO) and in general to cationic dyes.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; L11640; AAA35043.1; -; Genomic_DNA.
DR EMBL; U02077; AAA52190.1; -; Genomic_DNA.
DR EMBL; L24961; AAA65606.1; -; Genomic_DNA.
DR EMBL; X77765; CAA54805.1; -; Genomic_DNA.
DR EMBL; U25841; AAB64626.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11612.1; -; Genomic_DNA.
DR PIR; S40888; S40888.
DR RefSeq; NP_015524.1; NM_001184295.1.
DR AlphaFoldDB; P33335; -.
DR BioGRID; 36368; 71.
DR DIP; DIP-7575N; -.
DR IntAct; P33335; 3.
DR MINT; P33335; -.
DR STRING; 4932.YPR198W; -.
DR TCDB; 2.A.1.3.54; the major facilitator superfamily (mfs).
DR iPTMnet; P33335; -.
DR MaxQB; P33335; -.
DR PaxDb; P33335; -.
DR PRIDE; P33335; -.
DR EnsemblFungi; YPR198W_mRNA; YPR198W; YPR198W.
DR GeneID; 856327; -.
DR KEGG; sce:YPR198W; -.
DR SGD; S000006402; SGE1.
DR VEuPathDB; FungiDB:YPR198W; -.
DR eggNOG; KOG0254; Eukaryota.
DR GeneTree; ENSGT00940000176547; -.
DR HOGENOM; CLU_000960_22_1_1; -.
DR InParanoid; P33335; -.
DR OMA; IVMARIN; -.
DR BioCyc; YEAST:G3O-34318-MON; -.
DR PRO; PR:P33335; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; P33335; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:SGD.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IMP:SGD.
DR GO; GO:0055085; P:transmembrane transport; IMP:SGD.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..543
FT /note="Protein SGE1"
FT /id="PRO_0000173422"
FT TOPO_DOM 1..8
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..41
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 63..79
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 101..103
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..131
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..162
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..227
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 249..255
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 277..300
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 301..321
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 322..341
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 363..373
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 395..399
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 400..420
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 421..443
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 444..464
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 465..508
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 509..529
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 530..543
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 429
FT /note="D -> G (in Ref. 3; AAA65606)"
FT /evidence="ECO:0000305"
FT CONFLICT 447..486
FT /note="KSLGFAFGGNMGAMIFTASLKNQMRSSQLNIPQFTSVETL -> NPWALRLE
FT GYGGNDIHCITQKPDALFPIKHTTIYVCRNT (in Ref. 3; AAA65606)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 543 AA; 59426 MW; 16A3D91DA0B950FC CRC64;
MKSTLSLTLC VISLLLTLFL AALDIVIVVT LYDTIGIKFH DFGNIGWLVT GYALSNAVFM
LLWGRLAEIL GTKECLMISV IVFEIGSLIS ALSNSMATLI SGRVVAGFGG SGIESLAFVV
GTSIVRENHR GIMITALAIS YVIAEGVGPF IGGAFNEHLS WRWCFYINLP IGAFAFIILA
FCNTSGEPHQ KMWLPSKIKK IMNYDYGELL KASFWKNTFE VLVFKLDMVG IILSSAGFTL
LMLGLSFGGN NFPWNSGIII CFFTVGPILL LLFCAYDFHF LSLSGLHYDN KRIKPLLTWN
IASNCGIFTS SITGFLSCFA YELQSAYLVQ LYQLVFKKKP TLASIHLWEL SIPAMIATMA
IAYLNSKYGI IKPAIVFGVL CGIVGSGLFT LINGELSQSI GYSILPGIAF GSIFQATLLS
SQVQITSDDP DFQNKFIEVT AFNSFAKSLG FAFGGNMGAM IFTASLKNQM RSSQLNIPQF
TSVETLLAYS TEHYDGPQSS LSKFINTAIH DVFYCALGCY ALSFFFGIFT SSKKTTISAK
KQQ
