SGMR2_MOUSE
ID SGMR2_MOUSE Reviewed; 176 AA.
AC Q8VD00;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Sigma intracellular receptor 2 {ECO:0000250|UniProtKB:Q5BJF2};
DE Short=Sigma-2 receptor {ECO:0000250|UniProtKB:Q5BJF2};
DE Short=Sigma2 receptor {ECO:0000250|UniProtKB:Q5BJF2};
DE AltName: Full=Transmembrane protein 97;
GN Name=Tmem97; Synonyms=S2r {ECO:0000250|UniProtKB:Q5BJF2};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NOD; TISSUE=Dendritic cell, and Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Liver, and Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver, Pancreas, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Intracellular orphan receptor that binds numerous drugs and
CC which is highly expressed in various proliferating cells. Corresponds
CC to the sigma-2 receptor, which is thought to play important role in
CC regulating cell survival, morphology and differentiation. May play a
CC role as a regulator of cellular cholesterol homeostasis. May function
CC as sterol isomerase. May alter the activity of some cytochrome P450
CC proteins. {ECO:0000250|UniProtKB:Q5BJF2}.
CC -!- SUBUNIT: Interacts with NPC1. {ECO:0000250|UniProtKB:Q5BJF2}.
CC -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000250|UniProtKB:Q5BJF2};
CC Multi-pass membrane protein {ECO:0000255}. Rough endoplasmic reticulum
CC membrane {ECO:0000250|UniProtKB:Q5BJF2}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Localized at cell membrane and in lysosomes in
CC sterol-depleted cells when expression of endogenous TMEM97 is
CC stimulated. {ECO:0000250|UniProtKB:Q5BJF2}.
CC -!- MISCELLANEOUS: Sigma receptors are classified into two subtypes (Sigma-
CC 1 and Sigma-2) based on their different pharmacological profile. Sigma-
CC 2 receptors are identified by radioligand-binding studies as a binding
CC site with high affinity for di-o-tolylguanidine (DTG) and haloperidol.
CC {ECO:0000250|UniProtKB:Q5BJF2}.
CC -!- SIMILARITY: Belongs to the TMEM97/sigma-2 receptor family.
CC {ECO:0000305}.
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DR EMBL; AK155107; BAE33052.1; -; mRNA.
DR EMBL; AK171954; BAE42746.1; -; mRNA.
DR EMBL; AL591177; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC018156; AAH18156.1; -; mRNA.
DR EMBL; BC093511; AAH93511.1; -; mRNA.
DR CCDS; CCDS25112.1; -.
DR RefSeq; NP_598467.1; NM_133706.2.
DR AlphaFoldDB; Q8VD00; -.
DR SMR; Q8VD00; -.
DR STRING; 10090.ENSMUSP00000099532; -.
DR BindingDB; Q8VD00; -.
DR ChEMBL; CHEMBL4802011; -.
DR GuidetoPHARMACOLOGY; 2553; -.
DR iPTMnet; Q8VD00; -.
DR PhosphoSitePlus; Q8VD00; -.
DR jPOST; Q8VD00; -.
DR MaxQB; Q8VD00; -.
DR PaxDb; Q8VD00; -.
DR PeptideAtlas; Q8VD00; -.
DR PRIDE; Q8VD00; -.
DR ProteomicsDB; 261205; -.
DR TopDownProteomics; Q8VD00; -.
DR Antibodypedia; 50911; 103 antibodies from 11 providers.
DR DNASU; 69071; -.
DR Ensembl; ENSMUST00000103242; ENSMUSP00000099532; ENSMUSG00000037278.
DR GeneID; 69071; -.
DR KEGG; mmu:69071; -.
DR UCSC; uc007kjv.1; mouse.
DR CTD; 27346; -.
DR MGI; MGI:1916321; Tmem97.
DR VEuPathDB; HostDB:ENSMUSG00000037278; -.
DR eggNOG; ENOG502RZRX; Eukaryota.
DR GeneTree; ENSGT00390000007149; -.
DR HOGENOM; CLU_086812_1_0_1; -.
DR InParanoid; Q8VD00; -.
DR OMA; FYFASHI; -.
DR OrthoDB; 1459246at2759; -.
DR PhylomeDB; Q8VD00; -.
DR TreeFam; TF300241; -.
DR BioGRID-ORCS; 69071; 4 hits in 73 CRISPR screens.
DR ChiTaRS; Tmem97; mouse.
DR PRO; PR:Q8VD00; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8VD00; protein.
DR Bgee; ENSMUSG00000037278; Expressed in spermatocyte and 99 other tissues.
DR Genevisible; Q8VD00; MM.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR GO; GO:0031965; C:nuclear membrane; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0005791; C:rough endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0030867; C:rough endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042632; P:cholesterol homeostasis; ISS:UniProtKB.
DR InterPro; IPR033118; EXPERA.
DR InterPro; IPR016964; Sigma2_recept.
DR PIRSF; PIRSF031032; TMP_97_prd; 1.
DR PROSITE; PS51751; EXPERA; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Membrane; Nucleus; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..176
FT /note="Sigma intracellular receptor 2"
FT /id="PRO_0000254569"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..68
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 90..99
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..140
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 162..176
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 10..158
FT /note="EXPERA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01087"
SQ SEQUENCE 176 AA; 20811 MW; 245D2427FC03AE2E CRC64;
MGALAARRCV EWLLGLYFVS HIPITLFIDL QAVLPPELYP QEFSNLLRWY SKEFKDPLMQ
EPPVWFKSFL LCELVFQLPF FPIAAYAFFK GSCRWIRIPA IIYAAHTITT LIPILYTLLF
EDFSKAVAFK GQRPESFRER LTLVGVYAPY LIIPLILLLF MLRNPYYKYE EKRKKK
