ID   SGN1_YEAST              Reviewed;         250 AA.
AC   P40561; D6VVT1;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 167.
DE   RecName: Full=RNA-binding protein SGN1;
GN   Name=SGN1; Synonyms=RBP29; OrderedLocusNames=YIR001C; ORFNames=YIB1C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7762303; DOI=10.1002/yea.320110109;
RA   Voss H., Tamames J., Teodoru C., Valencia A., Sensen C., Wiemann S.,
RA   Schwager C., Zimmermann J., Sander C., Ansorge W.;
RT   "Nucleotide sequence and analysis of the centromeric region of yeast
RT   chromosome IX.";
RL   Yeast 11:61-78(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169870;
RA   Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA   Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA   Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA   Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA   Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL   Nature 387:84-87(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   RNA-BINDING, AND SUBCELLULAR LOCATION.
RX   PubMed=10764794; DOI=10.1074/jbc.m002412200;
RA   Winstall E., Sadowski M., Kuhn U., Wahle E., Sachs A.B.;
RT   "The Saccharomyces cerevisiae RNA-binding protein Rbp29 functions in
RT   cytoplasmic mRNA metabolism.";
RL   J. Biol. Chem. 275:21817-21826(2000).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: mRNA-binding protein that may play a role in modulating the
CC       expression of cytoplasmic mRNA.
CC   -!- INTERACTION:
CC       P40561; P32588: PUB1; NbExp=3; IntAct=EBI-25362, EBI-14231;
CC       P40561; P53316: YGR250C; NbExp=5; IntAct=EBI-25362, EBI-23586;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10764794}.
CC   -!- MISCELLANEOUS: Present with 6490 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; X79743; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; Z38062; CAA86203.1; -; Genomic_DNA.
DR   EMBL; BK006942; DAA08547.1; -; Genomic_DNA.
DR   PIR; S48435; S48435.
DR   RefSeq; NP_012266.1; NM_001179523.1.
DR   AlphaFoldDB; P40561; -.
DR   SMR; P40561; -.
DR   BioGRID; 34992; 159.
DR   DIP; DIP-6566N; -.
DR   IntAct; P40561; 18.
DR   MINT; P40561; -.
DR   STRING; 4932.YIR001C; -.
DR   iPTMnet; P40561; -.
DR   MaxQB; P40561; -.
DR   PaxDb; P40561; -.
DR   PRIDE; P40561; -.
DR   EnsemblFungi; YIR001C_mRNA; YIR001C; YIR001C.
DR   GeneID; 854817; -.
DR   KEGG; sce:YIR001C; -.
DR   SGD; S000001440; SGN1.
DR   VEuPathDB; FungiDB:YIR001C; -.
DR   eggNOG; KOG4209; Eukaryota.
DR   HOGENOM; CLU_1111890_0_0_1; -.
DR   InParanoid; P40561; -.
DR   OMA; ESKDEGC; -.
DR   BioCyc; YEAST:G3O-31422-MON; -.
DR   PRO; PR:P40561; -.
DR   Proteomes; UP000002311; Chromosome IX.
DR   RNAct; P40561; protein.
DR   GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR   GO; GO:0008143; F:poly(A) binding; IDA:SGD.
DR   GO; GO:0016071; P:mRNA metabolic process; IGI:SGD.
DR   Gene3D; 3.30.70.330; -; 1.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   Pfam; PF00076; RRM_1; 1.
DR   SMART; SM00360; RRM; 1.
DR   SUPFAM; SSF54928; SSF54928; 1.
DR   PROSITE; PS50102; RRM; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Reference proteome; RNA-binding.
FT   CHAIN           1..250
FT                   /note="RNA-binding protein SGN1"
FT                   /id="PRO_0000081956"
FT   DOMAIN          64..141
FT                   /note="RRM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   REGION          1..27
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          178..250
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        187..211
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        221..242
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   250 AA;  28954 MW;  373908F72DEB6873 CRC64;
     MSQEEKVDAK ATLKTEISNN KKNDKQELEL DELVGKLSIE GTPQVSQKLS KEEKHAHQLE
     ADSRSIFVGN ITPDVTPEQI EDHFKDCGQI KRITLLYDRN TGTPKGYGYI EFESPAYREK
     ALQLNGGELK GKKIAVSRKR TNIPGFNRHY NSQNQYFQQW QWNYPLMAYP NPDTFPYYPP
     YPPNQSPNQN FGYNKNNYYR SPYNNKNRTF QKKHFNSAKD STKNIRSTSQ KPVVMPSDNV
     KSSTQEKDSK