SGO1_CAEEL
ID SGO1_CAEEL Reviewed; 309 AA.
AC Q18412; W6SBE3;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2020, sequence version 3.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Shugoshin;
GN Name=sgo-1 {ECO:0000312|WormBase:C33H5.15b};
GN ORFNames=C33H5.15 {ECO:0000312|WormBase:C33H5.15b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP DISRUPTION PHENOTYPE, AND MUTAGENESIS OF 157-GLU--PHE-309.
RX PubMed=18923084; DOI=10.1101/gad.1691208;
RA de Carvalho C.E., Zaaijer S., Smolikov S., Gu Y., Schumacher J.M.,
RA Colaiacovo M.P.;
RT "LAB-1 antagonizes the Aurora B kinase in C. elegans.";
RL Genes Dev. 22:2869-2885(2008).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE,
RP AND MUTAGENESIS OF 157-GLU--PHE-309.
RX PubMed=30293721; DOI=10.1016/j.cub.2018.08.026;
RA Bohr T., Nelson C.R., Giacopazzi S., Lamelza P., Bhalla N.;
RT "Shugoshin is essential for meiotic prophase checkpoints in C. elegans.";
RL Curr. Biol. 28:3199-3211(2018).
CC -!- FUNCTION: Component of cell cycle checkpoints, which ensures chromosome
CC segregation during meiosis and mitosis (PubMed:18923084). During
CC meiotic prophase, it is involved in the regulation of the synapsis
CC checkpoint, which monitors whether homologous chromosomes have
CC synapsed, and the DNA damage response (PubMed:30293721). Plays a
CC central role in chromosome cohesion during cell division by preventing
CC premature dissociation of cohesin complex after prophase, when most of
CC cohesin complex dissociates from chromosomes arms (By similarity).
CC {ECO:0000250|UniProtKB:Q5FBB7, ECO:0000269|PubMed:18923084,
CC ECO:0000269|PubMed:30293721}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:30293721}.
CC Chromosome, centromere {ECO:0000269|PubMed:18923084}. Chromosome
CC {ECO:0000269|PubMed:18923084}. Note=Localizes to nuclei prior to entry
CC into meiotic prophase and late meiotic prophase (PubMed:30293721).
CC Localizes to the short arms of bivalent chromosomes from late
CC diakinesis until anaphase II (PubMed:18923084).
CC {ECO:0000269|PubMed:18923084, ECO:0000269|PubMed:30293721}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b {ECO:0000312|WormBase:C33H5.15b};
CC IsoId=Q18412-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:C33H5.15a};
CC IsoId=Q18412-2; Sequence=VSP_060484;
CC -!- TISSUE SPECIFICITY: Expressed in gonads. {ECO:0000269|PubMed:18923084,
CC ECO:0000269|PubMed:30293721}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryos.
CC {ECO:0000269|PubMed:18923084}.
CC -!- DISRUPTION PHENOTYPE: Viable with 100% of viable progeny
CC (PubMed:30293721). Defects in the regulation of the synapsis checkpoint
CC and DNA damage response in meiosis with an increased rate of
CC synaptonemal complex disassembly in late pachytene meiotic nuclei and
CC an increased number of rad-51 positive and hus-1 positive nuclei in
CC meiotic prophase (PubMed:30293721). Reduces the number of apoptotic
CC nuclei in the syp-1 mutant background, which is indicative of
CC inactivation of the synapsis checkpoint or the DNA damage response
CC (PubMed:30293721). RNAi-mediated knockdown results in chromosome
CC segregation defects during meiosis and mitosis (PubMed:18923084).
CC {ECO:0000269|PubMed:18923084, ECO:0000269|PubMed:30293721}.
CC -!- SIMILARITY: Belongs to the shugoshin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BX284604; CCD66579.1; -; Genomic_DNA.
DR EMBL; BX284604; CDM63523.1; -; Genomic_DNA.
DR PIR; T34148; T34148.
DR RefSeq; NP_001294168.1; NM_001307239.1. [Q18412-1]
DR RefSeq; NP_501290.2; NM_068889.4. [Q18412-2]
DR AlphaFoldDB; Q18412; -.
DR SMR; Q18412; -.
DR BioGRID; 48020; 2.
DR IntAct; Q18412; 2.
DR STRING; 6239.C33H5.15; -.
DR EPD; Q18412; -.
DR PaxDb; Q18412; -.
DR PeptideAtlas; Q18412; -.
DR EnsemblMetazoa; C33H5.15a.1; C33H5.15a.1; WBGene00016381. [Q18412-2]
DR EnsemblMetazoa; C33H5.15b.1; C33H5.15b.1; WBGene00016381. [Q18412-1]
DR EnsemblMetazoa; C33H5.15b.2; C33H5.15b.2; WBGene00016381. [Q18412-1]
DR GeneID; 183189; -.
DR KEGG; cel:CELE_C33H5.15; -.
DR UCSC; C33H5.15; c. elegans. [Q18412-1]
DR CTD; 183189; -.
DR WormBase; C33H5.15a; CE34732; WBGene00016381; sgo-1. [Q18412-2]
DR WormBase; C33H5.15b; CE49542; WBGene00016381; sgo-1. [Q18412-1]
DR eggNOG; ENOG502RT7Z; Eukaryota.
DR HOGENOM; CLU_858533_0_0_1; -.
DR InParanoid; Q18412; -.
DR OMA; DEPMPFI; -.
DR OrthoDB; 1385559at2759; -.
DR PRO; PR:Q18412; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00016381; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR InterPro; IPR011516; Shugoshin_N.
DR Pfam; PF07558; Shugoshin_N; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome;
KW Chromosome partition; Coiled coil; Nucleus; Reference proteome.
FT CHAIN 1..309
FT /note="Shugoshin"
FT /id="PRO_0000055442"
FT REGION 165..195
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 210..309
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 42..77
FT /evidence="ECO:0000255"
FT COMPBIAS 165..185
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 236..250
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 255..276
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 283..300
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 268..269
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_060484"
FT MUTAGEN 157..309
FT /note="Missing: In tm2443; chromosome segregation defects
FT during meiosis and mitosis."
FT /evidence="ECO:0000269|PubMed:18923084,
FT ECO:0000269|PubMed:30293721"
SQ SEQUENCE 309 AA; 34567 MW; D2951E740D04B447 CRC64;
MDAKTAQSIF GGIVAAKKRP SKEVPEPTIN FKSANDSLVK KNLLLKQQVV QCTKTIEKLR
NENVALRQKN QELIDGTLEQ RIELIVEQRL KFRLAHAAVL HKKLVQNIQQ TGLELDGLFK
DLEPEPSGLN TRRPPKLELN LERVDEIPFC QSSMQGEIDE NEIRFDNNSS QSTSSIQNAV
NGTPRKKQSV GKGRRSELFQ SFNADSSIVE EASTIPTNRR APMLIAPSST PAGPSKPTAR
KPPTPRFKKP STPALAPQSD DTELSSTIQV RRQRSAKMNI KSLKEPSGKD KLRRPGKHDE
PMPYINTFF
