SGO2_ARATH
ID SGO2_ARATH Reviewed; 470 AA.
AC Q0WTB8; A1L4U8;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=SHUGOSHIN 2 {ECO:0000303|PubMed:23884434};
DE Short=AtSGO2 {ECO:0000303|PubMed:23884434};
GN Name=SGO2 {ECO:0000303|PubMed:23884434};
GN OrderedLocusNames=At5g04320 {ECO:0000312|Araport:AT5G04320};
GN ORFNames=T19N18.50 {ECO:0000305};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION.
RX PubMed=24206843; DOI=10.1016/j.cub.2013.08.036;
RA Cromer L., Jolivet S., Horlow C., Chelysheva L., Heyman J., De Jaeger G.,
RA Koncz C., De Veylder L., Mercier R.;
RT "Centromeric cohesion is protected twice at meiosis, by SHUGOSHINs at
RT anaphase I and by PATRONUS at interkinesis.";
RL Curr. Biol. 23:2090-2099(2013).
RN [6]
RP FUNCTION.
RX PubMed=23884434; DOI=10.1007/s00497-013-0231-x;
RA Zamariola L., De Storme N., Tiang C.L., Armstrong S.J., Franklin F.C.,
RA Geelen D.;
RT "SGO1 but not SGO2 is required for maintenance of centromere cohesion in
RT Arabidopsis thaliana meiosis.";
RL Plant Reprod. 26:197-208(2013).
RN [7]
RP FUNCTION.
RX PubMed=24506176; DOI=10.1111/tpj.12432;
RA Zamariola L., De Storme N., Vannerum K., Vandepoele K., Armstrong S.J.,
RA Franklin F.C., Geelen D.;
RT "SHUGOSHINs and PATRONUS protect meiotic centromere cohesion in Arabidopsis
RT thaliana.";
RL Plant J. 77:782-794(2014).
CC -!- FUNCTION: Dispensable for both meiotic and mitotic cell cycle
CC progression (PubMed:24206843, PubMed:23884434). Required with SGO1 for
CC full protection of centromeric cohesion during anaphase I
CC (PubMed:24206843). Required to prevent precocious release of
CC pericentromeric cohesins during meiosis (PubMed:24206843). Acts
CC redundantly to SGO1 (PubMed:24506176). {ECO:0000269|PubMed:23884434,
CC ECO:0000269|PubMed:24206843, ECO:0000269|PubMed:24506176}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0WTB8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0WTB8-2; Sequence=VSP_058682;
CC -!- MISCELLANEOUS: Shugoshin is Japanese for guardian spirit.
CC {ECO:0000305|PubMed:24206843}.
CC -!- MISCELLANEOUS: Knock-down mutants show no meiotic or vegetative defects
CC and no reduced fertility (PubMed:24206843, PubMed:23884434). Sgo1 and
CC sgo2 double mutants are almost completely sterile and show a premature
CC separation of sister chromatids at anaphase I (PubMed:24506176).
CC {ECO:0000269|PubMed:23884434, ECO:0000269|PubMed:24206843,
CC ECO:0000269|PubMed:24506176}.
CC -!- SIMILARITY: Belongs to the shugoshin family. {ECO:0000305}.
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DR EMBL; CP002688; AED90726.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90727.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70336.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70337.1; -; Genomic_DNA.
DR EMBL; AK227641; BAE99630.1; -; mRNA.
DR EMBL; BT029735; ABM06005.1; -; mRNA.
DR RefSeq; NP_001078526.1; NM_001085057.2. [Q0WTB8-1]
DR RefSeq; NP_001331955.1; NM_001342765.1. [Q0WTB8-1]
DR RefSeq; NP_001331956.1; NM_001342764.1. [Q0WTB8-1]
DR RefSeq; NP_196052.2; NM_120514.5. [Q0WTB8-2]
DR AlphaFoldDB; Q0WTB8; -.
DR SMR; Q0WTB8; -.
DR IntAct; Q0WTB8; 1.
DR STRING; 3702.AT5G04320.2; -.
DR PaxDb; Q0WTB8; -.
DR PRIDE; Q0WTB8; -.
DR ProteomicsDB; 234519; -. [Q0WTB8-1]
DR EnsemblPlants; AT5G04320.1; AT5G04320.1; AT5G04320. [Q0WTB8-2]
DR EnsemblPlants; AT5G04320.2; AT5G04320.2; AT5G04320. [Q0WTB8-1]
DR EnsemblPlants; AT5G04320.4; AT5G04320.4; AT5G04320. [Q0WTB8-1]
DR EnsemblPlants; AT5G04320.5; AT5G04320.5; AT5G04320. [Q0WTB8-1]
DR GeneID; 830311; -.
DR Gramene; AT5G04320.1; AT5G04320.1; AT5G04320. [Q0WTB8-2]
DR Gramene; AT5G04320.2; AT5G04320.2; AT5G04320. [Q0WTB8-1]
DR Gramene; AT5G04320.4; AT5G04320.4; AT5G04320. [Q0WTB8-1]
DR Gramene; AT5G04320.5; AT5G04320.5; AT5G04320. [Q0WTB8-1]
DR KEGG; ath:AT5G04320; -.
DR Araport; AT5G04320; -.
DR TAIR; locus:2179954; AT5G04320.
DR eggNOG; ENOG502RYDC; Eukaryota.
DR InParanoid; Q0WTB8; -.
DR OMA; MRIDANK; -.
DR OrthoDB; 947253at2759; -.
DR PhylomeDB; Q0WTB8; -.
DR PRO; PR:Q0WTB8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q0WTB8; baseline and differential.
DR GO; GO:0000775; C:chromosome, centromeric region; IEA:InterPro.
DR GO; GO:0005634; C:nucleus; IEA:InterPro.
DR GO; GO:0034090; P:maintenance of meiotic sister chromatid cohesion; IGI:TAIR.
DR InterPro; IPR044693; SGO_plant.
DR InterPro; IPR011515; Shugoshin_C.
DR PANTHER; PTHR34373; PTHR34373; 2.
DR Pfam; PF07557; Shugoshin_C; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chromosome partition; Coiled coil;
KW Reference proteome.
FT CHAIN 1..470
FT /note="SHUGOSHIN 2"
FT /id="PRO_0000438547"
FT REGION 131..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 358..470
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 72..113
FT /evidence="ECO:0000255"
FT COMPBIAS 131..147
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 367..385
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 420..439
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 445..463
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..51
FT /note="Missing (in isoform 2)"
FT /id="VSP_058682"
SQ SEQUENCE 470 AA; 53706 MW; 3E75F9FE275F5B53 CRC64;
MVLTEMSLDY SNRVLGSQKD NVKQMDKEET QQKENMLFSS QEYAAKLQKE NMTLMKALAH
RNKLVELSGI EIQKLRINLR SVQEKNLQLA QANSQMLAEL NTNRDRLKDL QHELGCKNAL
LKVKKHLEEQ VLPRTHHESK DKVSASASDG DCKSFQVHDI KHKDTKRKRT TRIKSSVSAD
VKPIPVNDSN SKANRKRRVS GVIDTTGIPE EICQTEDDID KGVVSRGVNQ DIDNVVNKKF
VPDAANPVKE SVHRKRQCTR RQSTRFDVQE TKQTEKLLEM DGAKESKETA SFSLRRRSAR
LRHEEAEPCK SLHEGDEVRE TIKRRRVSLR LSARFDIQEP HVTETSNADD ARSIVIEESA
GSRSESVEPS ESRHETKEIT RKRSFSTRRQ STKGKSQTDE AIKEIATDPS LVNTIVQECD
QETESKDKPK ADENEGMTRR SSVGRPSRHA AEKVQSYREV SLRVKMRRKC
