SGR1_ARATH
ID SGR1_ARATH Reviewed; 268 AA.
AC O82741; Q8LBZ2;
DT 19-FEB-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Magnesium dechelatase SGR1, chloroplastic {ECO:0000305};
DE EC=4.99.1.10 {ECO:0000269|PubMed:27604697};
DE AltName: Full=Protein NONYELLOWING 1 {ECO:0000303|PubMed:17468209};
DE Short=AtNYE1 {ECO:0000303|PubMed:17468209};
DE AltName: Full=Protein STAY-GREEN 1 {ECO:0000303|PubMed:22366162};
DE AltName: Full=Protein STAYGREEN 1 {ECO:0000303|PubMed:17513504};
DE Flags: Precursor;
GN Name=SGR1 {ECO:0000303|PubMed:24043799};
GN Synonyms=NYE1 {ECO:0000303|PubMed:17468209},
GN SGN1 {ECO:0000303|PubMed:17513504};
GN OrderedLocusNames=At4g22920 {ECO:0000312|Araport:AT4G22920};
GN ORFNames=F7H19.100 {ECO:0000312|EMBL:CAA19807.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=17513504; DOI=10.1105/tpc.106.044891;
RA Park S.Y., Yu J.W., Park J.S., Li J., Yoo S.C., Lee N.Y., Lee S.K.,
RA Jeong S.W., Seo H.S., Koh H.J., Jeon J.S., Park Y.I., Paek N.C.;
RT "The senescence-induced staygreen protein regulates chlorophyll
RT degradation.";
RL Plant Cell 19:1649-1664(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, INDUCTION BY DARK, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=17468209; DOI=10.1104/pp.107.100172;
RA Ren G., An K., Liao Y., Zhou X., Cao Y., Zhao H., Ge X., Kuai B.;
RT "Identification of a novel chloroplast protein AtNYE1 regulating
RT chlorophyll degradation during leaf senescence in Arabidopsis.";
RL Plant Physiol. 144:1429-1441(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=17204643; DOI=10.1126/science.1132912;
RA Armstead I., Donnison I., Aubry S., Harper J., Hortensteiner S., James C.,
RA Mani J., Moffet M., Ougham H., Roberts L., Thomas A., Weeden N., Thomas H.,
RA King I.;
RT "Cross-species identification of Mendel's I locus.";
RL Science 315:73-73(2007).
RN [8]
RP DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX DOI=10.1016/j.plantsci.2008.12.013;
RA Barry C.S.;
RT "The stay-green revolution: Recent progress in deciphering the mechanisms
RT of chlorophyll degradation in higher plants.";
RL Plant Sci. 176:325-333(2009).
RN [9]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH NYC1; PAO; RCCR AND LHCII
RP COMPLEX.
RX PubMed=22366162; DOI=10.1105/tpc.111.089474;
RA Sakuraba Y., Schelbert S., Park S.Y., Han S.H., Lee B.D., Andres C.B.,
RA Kessler F., Hortensteiner S., Paek N.C.;
RT "STAY-GREEN and chlorophyll catabolic enzymes interact at light-harvesting
RT complex II for chlorophyll detoxification during leaf senescence in
RT Arabidopsis.";
RL Plant Cell 24:507-518(2012).
RN [10]
RP INTERACTION WITH HCAR, AND DEVELOPMENTAL STAGE.
RX PubMed=23200839; DOI=10.1016/j.bbrc.2012.11.050;
RA Sakuraba Y., Kim Y.S., Yoo S.C., Hortensteiner S., Paek N.C.;
RT "7-Hydroxymethyl chlorophyll a reductase functions in metabolic channeling
RT of chlorophyll breakdown intermediates during leaf senescence.";
RL Biochem. Biophys. Res. Commun. 430:32-37(2013).
RN [11]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=24043799; DOI=10.1073/pnas.1308114110;
RA Delmas F., Sankaranarayanan S., Deb S., Widdup E., Bournonville C.,
RA Bollier N., Northey J.G., McCourt P., Samuel M.A.;
RT "ABI3 controls embryo degreening through Mendel's I locus.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E3888-E3894(2013).
RN [12]
RP INDUCTION.
RX PubMed=24719469; DOI=10.1093/mp/ssu045;
RA Sakuraba Y., Park S.Y., Kim Y.S., Wang S.H., Yoo S.C., Hoertensteiner S.,
RA Paek N.C.;
RT "Arabidopsis STAY-GREEN2 is a negative regulator of chlorophyll degradation
RT during leaf senescence.";
RL Mol. Plant 7:1288-1302(2014).
RN [13]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=27604697; DOI=10.1105/tpc.16.00428;
RA Shimoda Y., Ito H., Tanaka A.;
RT "Arabidopsis STAY-GREEN, Mendel's green cotyledon gene, encodes magnesium-
RT dechelatase.";
RL Plant Cell 28:2147-2160(2016).
RN [14]
RP FUNCTION.
RX PubMed=28873256; DOI=10.1111/tpj.13710;
RA Li Z., Wu S., Chen J., Wang X., Gao J., Ren G., Kuai B.;
RT "NYEs/SGRs-mediated chlorophyll degradation is critical for detoxification
RT during seed maturation in Arabidopsis.";
RL Plant J. 92:650-661(2017).
RN [15]
RP FUNCTION.
RX PubMed=29425814; DOI=10.1016/j.jplph.2018.01.010;
RA Sato T., Shimoda Y., Matsuda K., Tanaka A., Ito H.;
RT "Mg-dechelation of chlorophyll a by Stay-Green activates chlorophyll b
RT degradation through expressing Non-Yellow Coloring 1 in Arabidopsis
RT thaliana.";
RL J. Plant Physiol. 222:94-102(2018).
CC -!- FUNCTION: Magnesium chelatase involved in chlorophyll a degradation in
CC the chlorophyll-protein complexes of photosystem I (PSI) and
CC photosystem II (PSII) (PubMed:27604697). Contributes to the degradation
CC of PSI and PSII in the thylakoid membranes (PubMed:27604697). Required
CC to trigger chlorophyll degradation during natural and dark-induced leaf
CC senescence (Probable) (PubMed:17468209). Mediates chlorophyll
CC degradation during embryo degreening (PubMed:24043799,
CC PubMed:28873256). Recombinant SGR1 possesses high dechelating activity
CC against chlorophyll a, very low activity against chlorophyllide a, and
CC no activity against chlorophyll b (PubMed:27604697). Magnesium
CC dechelation of chlorophyll a by SGR1 activates chlorophyll b
CC degradation by inducing the expression of NYC1, an enzyme involved in
CC chlorophyll b degradation (PubMed:29425814).
CC {ECO:0000269|PubMed:17468209, ECO:0000269|PubMed:24043799,
CC ECO:0000269|PubMed:27604697, ECO:0000269|PubMed:28873256,
CC ECO:0000269|PubMed:29425814, ECO:0000305|PubMed:17513504}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chlorophyll a + 2 H(+) = Mg(2+) + pheophytin a;
CC Xref=Rhea:RHEA:52788, ChEBI:CHEBI:15378, ChEBI:CHEBI:18420,
CC ChEBI:CHEBI:58416, ChEBI:CHEBI:136840; EC=4.99.1.10;
CC Evidence={ECO:0000269|PubMed:27604697};
CC -!- SUBUNIT: Interacts with HCAR, the chlorophyll catabolic enzymes (CCEs)
CC NYC1, PAO and RCCR, and the LHCII complex. Part of a SGR1-CCE-LHCII
CC complex, which acts in chlorophyll breakdown.
CC {ECO:0000269|PubMed:22366162, ECO:0000269|PubMed:23200839}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:17468209, ECO:0000269|PubMed:22366162}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, seeds, flowers, buds,
CC petals, sepals and siliques. {ECO:0000269|PubMed:17204643,
CC ECO:0000269|PubMed:17468209, ECO:0000269|Ref.8}.
CC -!- DEVELOPMENTAL STAGE: Constitutively expressed at low level during leaf
CC development, but up-regulated during seed maturation and senescence,
CC when the leaf color changes from green to yellow.
CC {ECO:0000269|PubMed:17204643, ECO:0000269|PubMed:17468209,
CC ECO:0000269|PubMed:17513504, ECO:0000269|PubMed:23200839,
CC ECO:0000269|PubMed:24043799, ECO:0000269|Ref.8}.
CC -!- INDUCTION: Up-regulated by dark treatment (PubMed:17468209). Induced
CC during natural and dark-induced leaf senescence (PubMed:24719469).
CC {ECO:0000269|PubMed:17468209, ECO:0000269|PubMed:24719469}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockout of the protein results in
CC a stay-green leaf phenotype. No effect on seed degreening; probably due
CC to redundancy with SGR1. Sgr1 and sgr2 double mutant has an embryo
CC stay-green phenotype. {ECO:0000269|PubMed:17204643}.
CC -!- SIMILARITY: Belongs to the staygreen family. {ECO:0000305}.
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DR EMBL; AY850161; AAW82962.1; -; mRNA.
DR EMBL; DQ437531; ABD77557.1; -; mRNA.
DR EMBL; AL031018; CAA19807.1; -; Genomic_DNA.
DR EMBL; AL161558; CAB79247.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84681.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM67141.1; -; Genomic_DNA.
DR EMBL; AY063900; AAL36256.1; -; mRNA.
DR EMBL; AY096504; AAM20154.1; -; mRNA.
DR EMBL; AY086912; AAM64476.1; -; mRNA.
DR PIR; T05123; T05123.
DR RefSeq; NP_001328989.1; NM_001341565.1.
DR RefSeq; NP_567673.1; NM_118421.4.
DR AlphaFoldDB; O82741; -.
DR BioGRID; 13680; 9.
DR IntAct; O82741; 5.
DR MINT; O82741; -.
DR STRING; 3702.AT4G22920.1; -.
DR PaxDb; O82741; -.
DR PRIDE; O82741; -.
DR ProteomicsDB; 232633; -.
DR DNASU; 828391; -.
DR EnsemblPlants; AT4G22920.1; AT4G22920.1; AT4G22920.
DR EnsemblPlants; AT4G22920.2; AT4G22920.2; AT4G22920.
DR GeneID; 828391; -.
DR Gramene; AT4G22920.1; AT4G22920.1; AT4G22920.
DR Gramene; AT4G22920.2; AT4G22920.2; AT4G22920.
DR KEGG; ath:AT4G22920; -.
DR Araport; AT4G22920; -.
DR TAIR; locus:2127153; AT4G22920.
DR eggNOG; ENOG502S3TG; Eukaryota.
DR HOGENOM; CLU_073517_0_0_1; -.
DR InParanoid; O82741; -.
DR OMA; EECSCCF; -.
DR OrthoDB; 1194276at2759; -.
DR PhylomeDB; O82741; -.
DR BioCyc; ARA:MONQT-8230; -.
DR BioCyc; MetaCyc:MON-20118; -.
DR PRO; PR:O82741; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O82741; baseline and differential.
DR Genevisible; O82741; AT.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0015996; P:chlorophyll catabolic process; IMP:TAIR.
DR InterPro; IPR024438; Staygreen.
DR Pfam; PF12638; Staygreen; 1.
PE 1: Evidence at protein level;
KW Chlorophyll catabolism; Chloroplast; Lyase; Membrane; Plastid;
KW Reference proteome; Thylakoid; Transit peptide.
FT TRANSIT 1..48
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 49..268
FT /note="Magnesium dechelatase SGR1, chloroplastic"
FT /id="PRO_0000425231"
SQ SEQUENCE 268 AA; 30052 MW; 1B3F128F18EA79B2 CRC64;
MCSLSAIMLL PTKLKPAYSD KRSNSSSSSS LFFNNRRSKK KNQSIVPVAR LFGPAIFESS
KLKVLFLGVD EKKHPSTLPR TYTLTHSDIT AKLTLAISQS INNSQLQGWA NRLYRDEVVA
EWKKVKGKMS LHVHCHISGG HFLLDLFAKF RYFIFCKELP VVLKAFVHGD GNLLNNYPEL
QEALVWVYFH SNVNEFNKVE CWGPLWEAVS PDGHKTETLP EARCADECSC CFPTVSSIPW
SHSLSNEGVN GYSGTQTEGI ATPNPEKL
