SGRL_ARATH
ID SGRL_ARATH Reviewed; 260 AA.
AC Q94AQ9; Q9LNZ8;
DT 19-FEB-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Magnesium dechelatase SGRL, chloroplastic {ECO:0000305};
DE EC=4.99.1.10 {ECO:0000269|PubMed:27604697};
DE AltName: Full=Protein STAY-GREEN LIKE {ECO:0000303|PubMed:25261252};
DE Flags: Precursor;
GN Name=SGRL {ECO:0000303|PubMed:25261252};
GN OrderedLocusNames=At1g44000 {ECO:0000312|Araport:AT1G44000};
GN ORFNames=F9C16.20 {ECO:0000312|EMBL:AAF79680.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP TISSUE SPECIFICITY.
RX DOI=10.1016/j.plantsci.2008.12.013;
RA Barry C.S.;
RT "The stay-green revolution: Recent progress in deciphering the mechanisms
RT of chlorophyll degradation in higher plants.";
RL Plant Sci. 176:325-333(2009).
RN [5]
RP FUNCTION, INTERACTION WITH LHCII; NYC1; NOL; PAO AND RCCR, AND SUBCELLULAR
RP LOCATION.
RX PubMed=25261252; DOI=10.1016/j.febslet.2014.09.018;
RA Sakuraba Y., Kim D., Kim Y.S., Hoertensteiner S., Paek N.C.;
RT "Arabidopsis STAYGREEN-LIKE (SGRL) promotes abiotic stress-induced leaf
RT yellowing during vegetative growth.";
RL FEBS Lett. 588:3830-3837(2014).
RN [6]
RP INDUCTION.
RX PubMed=24719469; DOI=10.1093/mp/ssu045;
RA Sakuraba Y., Park S.Y., Kim Y.S., Wang S.H., Yoo S.C., Hoertensteiner S.,
RA Paek N.C.;
RT "Arabidopsis STAY-GREEN2 is a negative regulator of chlorophyll degradation
RT during leaf senescence.";
RL Mol. Plant 7:1288-1302(2014).
RN [7]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=27604697; DOI=10.1105/tpc.16.00428;
RA Shimoda Y., Ito H., Tanaka A.;
RT "Arabidopsis STAY-GREEN, Mendel's green cotyledon gene, encodes magnesium-
RT dechelatase.";
RL Plant Cell 28:2147-2160(2016).
CC -!- FUNCTION: Magnesium chelatase involved in chlorophyll a degradation in
CC the chlorophyll-protein complexes of photosystem I (PSI) and
CC photosystem II (PSII) (PubMed:27604697). Contributes to the degradation
CC of PSI and PSII in the thylakoid membranes (PubMed:27604697).
CC Recombinant SGRL possesses high dechelating activity against
CC chlorophyllide a, very low activity against chlorophyll a, and no
CC activity against chlorophyll b (PubMed:27604697). Contributes to
CC abiotic stress-induced chlorophyll degradation and leaf yellowing
CC during vegetative plant growth (PubMed:25261252).
CC {ECO:0000269|PubMed:25261252, ECO:0000269|PubMed:27604697}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chlorophyllide a + 2 H(+) = Mg(2+) + pheophorbide a;
CC Xref=Rhea:RHEA:52792, ChEBI:CHEBI:15378, ChEBI:CHEBI:18420,
CC ChEBI:CHEBI:58687, ChEBI:CHEBI:83348; EC=4.99.1.10;
CC Evidence={ECO:0000269|PubMed:27604697};
CC -!- SUBUNIT: Interacts with the light harvesting complex II (LHCII)
CC (PubMed:25261252). Interacts with the chlorophyll catabolic enzymes
CC (CCEs) NYC1, NOL, PAO and RCCR (PubMed:25261252).
CC {ECO:0000269|PubMed:25261252}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid
CC {ECO:0000269|PubMed:25261252}.
CC -!- TISSUE SPECIFICITY: Expressed in cotyledons, pollen and young leaves.
CC {ECO:0000269|Ref.4}.
CC -!- INDUCTION: Down-regulated during natural and dark-induced leaf
CC senescence. {ECO:0000269|PubMed:24719469}.
CC -!- SIMILARITY: Belongs to the staygreen family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79680.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC022314; AAF79680.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE32011.1; -; Genomic_DNA.
DR EMBL; AY045870; AAK76544.1; -; mRNA.
DR EMBL; AY091453; AAM14392.1; -; mRNA.
DR RefSeq; NP_564489.1; NM_103526.3.
DR AlphaFoldDB; Q94AQ9; -.
DR BioGRID; 26225; 8.
DR IntAct; Q94AQ9; 7.
DR MINT; Q94AQ9; -.
DR STRING; 3702.AT1G44000.1; -.
DR PaxDb; Q94AQ9; -.
DR PRIDE; Q94AQ9; -.
DR EnsemblPlants; AT1G44000.1; AT1G44000.1; AT1G44000.
DR GeneID; 841000; -.
DR Gramene; AT1G44000.1; AT1G44000.1; AT1G44000.
DR KEGG; ath:AT1G44000; -.
DR Araport; AT1G44000; -.
DR TAIR; locus:2037154; AT1G44000.
DR eggNOG; ENOG502QS93; Eukaryota.
DR HOGENOM; CLU_073517_1_0_1; -.
DR InParanoid; Q94AQ9; -.
DR OMA; PFPRAYT; -.
DR OrthoDB; 1194276at2759; -.
DR PhylomeDB; Q94AQ9; -.
DR BioCyc; ARA:AT1G44000-MON; -.
DR BioCyc; MetaCyc:MON-20119; -.
DR PRO; PR:Q94AQ9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q94AQ9; baseline and differential.
DR Genevisible; Q94AQ9; AT.
DR GO; GO:0009534; C:chloroplast thylakoid; IEA:UniProtKB-SubCell.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0015996; P:chlorophyll catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0009658; P:chloroplast organization; IMP:TAIR.
DR InterPro; IPR024438; Staygreen.
DR Pfam; PF12638; Staygreen; 1.
PE 1: Evidence at protein level;
KW Chlorophyll catabolism; Chloroplast; Lyase; Plastid; Reference proteome;
KW Stress response; Thylakoid; Transit peptide.
FT TRANSIT 1..45
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 46..260
FT /note="Magnesium dechelatase SGRL, chloroplastic"
FT /id="PRO_0000425233"
SQ SEQUENCE 260 AA; 29892 MW; A0A75BCF7A145C00 CRC64;
MACYIVPYYH HPVLSHPNRE IFSHRHHHHH RFCNNLLNRR ISVPRSSAIS DGGVSYNTLV
SEAVRLLVPQ ANFDSSKLKV EFLGELLENK SNGGIITPRT YILSHCDFTA NLTLTISNVI
NLDQLEGWYK KDDVVAEWKK VNDELRLHIH CCVSGMSLLQ DVAAELRYHI FSKELPLVLK
AVVHGDSVMF RENPELMDAY VWVYFHSSTP KYNRIECWGP LKDAAKGKQQ GNHQGFLSST
TSRKLIRHKS IFHTLFTFLL
