SGS3_DROME
ID SGS3_DROME Reviewed; 307 AA.
AC P02840; Q9VTJ2;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Salivary glue protein Sgs-3 {ECO:0000305};
DE Flags: Precursor;
GN Name=Sgs3 {ECO:0000312|FlyBase:FBgn0003373};
GN ORFNames=CG11720 {ECO:0000312|FlyBase:FBgn0003373};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6411930; DOI=10.1016/s0022-2836(83)80074-6;
RA Garfinkel M.D., Pruitt R.E., Meyerowitz E.M.;
RT "DNA sequences, gene regulation and modular protein evolution in the
RT Drosophila 68C glue gene cluster.";
RL J. Mol. Biol. 168:765-789(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
RX PubMed=3138416; DOI=10.1016/0022-2836(88)90138-6;
RA Martin C.H., Mayeda C.A., Meyerowitz E.M.;
RT "Evolution and expression of the Sgs-3 glue gene of Drosophila.";
RL J. Mol. Biol. 201:273-287(1988).
RN [5]
RP DEVELOPMENTAL STAGE.
RX PubMed=8223281; DOI=10.1242/dev.118.2.613;
RA Huet F., Ruiz C., Richards G.;
RT "Puffs and PCR: the in vivo dynamics of early gene expression during
RT ecdysone responses in Drosophila.";
RL Development 118:613-627(1993).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, AND GLYCOSYLATION.
RX PubMed=30158631; DOI=10.1038/s41467-018-05978-9;
RA Ji S., Samara N.L., Revoredo L., Zhang L., Tran D.T., Muirhead K.,
RA Tabak L.A., Ten Hagen K.G.;
RT "A molecular switch orchestrates enzyme specificity and secretory granule
RT morphology.";
RL Nat. Commun. 9:3508-3508(2018).
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Specifically expressed in the salivary gland.
CC {ECO:0000269|PubMed:30158631}.
CC -!- DEVELOPMENTAL STAGE: In the salivary glands of mid instar larvae levels
CC dramatically increase during puff stage 1 at 98-106 hours of
CC development. Levels remain constant and abundant in late larvae until
CC puff stage 10, then decrease by stage 11. {ECO:0000269|PubMed:8223281}.
CC -!- PTM: O-glycosylated by Pgnat9 in salivary glands.
CC {ECO:0000269|PubMed:30158631}.
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DR EMBL; X01918; CAA25994.1; -; Genomic_DNA.
DR EMBL; AE014296; AAF50056.1; -; Genomic_DNA.
DR EMBL; X78392; CAA55154.1; -; Genomic_DNA.
DR PIR; A03329; GSFF3.
DR RefSeq; NP_524024.1; NM_079300.3.
DR AlphaFoldDB; P02840; -.
DR BioGRID; 64658; 1.
DR STRING; 7227.FBpp0075827; -.
DR PaxDb; P02840; -.
DR EnsemblMetazoa; FBtr0076096; FBpp0075827; FBgn0003373.
DR GeneID; 39288; -.
DR KEGG; dme:Dmel_CG11720; -.
DR CTD; 39288; -.
DR FlyBase; FBgn0003373; Sgs3.
DR VEuPathDB; VectorBase:FBgn0003373; -.
DR eggNOG; ENOG502TF7P; Eukaryota.
DR HOGENOM; CLU_834891_0_0_1; -.
DR InParanoid; P02840; -.
DR OMA; TTPEPCT; -.
DR OrthoDB; 1648653at2759; -.
DR BioGRID-ORCS; 39288; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Sgs3; fly.
DR GenomeRNAi; 39288; -.
DR PRO; PR:P02840; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0003373; Expressed in saliva-secreting gland and 10 other tissues.
DR Genevisible; P02840; DM.
DR GO; GO:0062130; C:adhesive extracellular matrix; IDA:FlyBase.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005201; F:extracellular matrix structural constituent; ISM:FlyBase.
DR GO; GO:0007594; P:puparial adhesion; IEP:FlyBase.
PE 1: Evidence at protein level;
KW Glycoprotein; Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..307
FT /note="Salivary glue protein Sgs-3"
FT /id="PRO_0000022329"
FT REGION 56..257
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 307 AA; 32196 MW; 45803DED16C418BC CRC64;
MKLTIATALA SILLIGSANV ANCCDCGCPT TTTTCAPRTT QPPCTTTTTT TTTTCAPPTQ
QSTTQPPCTT SKPTTPKQTT TQLPCTTPTT TKATTTKPTT TKATTTKATT TKPTTTKQTT
TQLPCTTPTT TKQTTTQLPC TTPTTTKPTT TKPTTTKPTT TKPTTTKPTT TKPTTTKPTT
TKPTTTKPTT TKPTTTKPTT TKPTTTKPTT TKPTTTKPTT TKPTTTKPTT TKPTTTKPTT
TKPTTTKPTT TKPTTPKPCG CKSCGPGGEP CNGCAKRDAL CQDLNGVLRN LERKIRQCVC
GEPQWLL
