ID   SGS3_MAIZE              Reviewed;         594 AA.
AC   A1Y2B7;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Protein SUPPRESSOR OF GENE SILENCING 3 homolog;
DE   AltName: Full=Protein LEAFBLADELESS 1;
DE   AltName: Full=ZmSGS3;
GN   Name=SGS3; Synonyms=LBL1;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF CYS-196 AND HIS-230, AND TISSUE
RP   SPECIFICITY.
RC   STRAIN=cv. B73;
RX   PubMed=17403777; DOI=10.1101/gad.1528607;
RA   Nogueira F.T., Madi S., Chitwood D.H., Juarez M.T., Timmermans M.C.P.;
RT   "Two small regulatory RNAs establish opposing fates of a developmental
RT   axis.";
RL   Genes Dev. 21:750-755(2007).
RN   [2]
RP   FUNCTION.
RX   PubMed=9655804; DOI=10.1242/dev.125.15.2813;
RA   Timmermans M.C.P., Schultes N.P., Jankovsky J.P., Nelson T.;
RT   "Leafbladeless1 is required for dorsoventrality of lateral organs in
RT   maize.";
RL   Development 125:2813-2823(1998).
CC   -!- FUNCTION: Required for the biogenesis of miRNAs and trans-acting siRNAs
CC       and essential for the specification of adaxial/abaxial organ polarity.
CC       May be involved in natural virus resistance (By similarity).
CC       {ECO:0000250, ECO:0000269|PubMed:9655804}.
CC   -!- TISSUE SPECIFICITY: Expressed in a dome of cells at the tip of the
CC       meristem that extends into the adaxial side of the initiating
CC       primordium. During the later stages of leaf development, expression
CC       becomes restricted to the margin and vasculature.
CC       {ECO:0000269|PubMed:17403777}.
CC   -!- SIMILARITY: Belongs to the SGS3 family. {ECO:0000305}.
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DR   EMBL; DQ832257; ABH10613.1; -; mRNA.
DR   RefSeq; NP_001105989.1; NM_001112519.1.
DR   AlphaFoldDB; A1Y2B7; -.
DR   SMR; A1Y2B7; -.
DR   STRING; 4577.GRMZM2G020187_P01; -.
DR   PaxDb; A1Y2B7; -.
DR   PRIDE; A1Y2B7; -.
DR   EnsemblPlants; Zm00001eb264310_T001; Zm00001eb264310_P001; Zm00001eb264310.
DR   GeneID; 100037819; -.
DR   Gramene; Zm00001eb264310_T001; Zm00001eb264310_P001; Zm00001eb264310.
DR   KEGG; zma:100037819; -.
DR   MaizeGDB; 12602; -.
DR   eggNOG; ENOG502QPU5; Eukaryota.
DR   HOGENOM; CLU_020338_1_0_1; -.
DR   OMA; DFERMQQ; -.
DR   OrthoDB; 370807at2759; -.
DR   Proteomes; UP000007305; Chromosome 6.
DR   ExpressionAtlas; A1Y2B7; baseline and differential.
DR   Genevisible; A1Y2B7; ZM.
DR   GO; GO:0051607; P:defense response to virus; IEA:InterPro.
DR   GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR   CDD; cd12266; RRM_like_XS; 1.
DR   Gene3D; 3.30.70.2890; -; 1.
DR   InterPro; IPR044287; SGS3.
DR   InterPro; IPR005380; XS_domain.
DR   InterPro; IPR038588; XS_domain_sf.
DR   InterPro; IPR005381; Znf-XS_domain.
DR   PANTHER; PTHR46602; PTHR46602; 1.
DR   Pfam; PF03468; XS; 1.
DR   Pfam; PF03470; zf-XS; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Developmental protein; Reference proteome;
KW   RNA-mediated gene silencing.
FT   CHAIN           1..594
FT                   /note="Protein SUPPRESSOR OF GENE SILENCING 3 homolog"
FT                   /id="PRO_0000333293"
FT   REGION          1..163
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          408..509
FT                   /evidence="ECO:0000255"
FT   COILED          545..577
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        36..67
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        132..155
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         196
FT                   /note="C->Y: In lbl1-rgd1; complete abaxalization of the
FT                   leaf."
FT                   /evidence="ECO:0000269|PubMed:17403777"
FT   MUTAGEN         230
FT                   /note="H->Y: In lbl1-372; complete abaxalization of the
FT                   leaf."
FT                   /evidence="ECO:0000269|PubMed:17403777"
SQ   SEQUENCE   594 AA;  67753 MW;  9F777970C41A6EAD CRC64;
     MSGSGDRRGG GPPGSHSGWE TMGKKSKKPG QAGGRQWAPW SSTNVTPNTA RPAWGGSGSS
     HPSGTSWAQA PDHGAATRGN PRPPSQTSRP VLAPPLANGW QWQSRPRPSG SEVKKDDAPP
     SGSVPEVENV DGNNTSDDDD DDDDDLSDDI SDDYDSDASE KSFETRKTNK WFKEFFEVLN
     TLSLEQINEQ TRQWHCPACK NGPGAIDWYK GLQPLVSHAR TKGSTRVKLH RELAALLEEE
     LSRRGTSVLP AGEQFGKWKG LQESTDREIV WPPMVIVMNT FLEKDEDDKW KGMGNQELLD
     YFGEYEASKA RHAYGPSGHR GMSVLIFESS AVGYMEAERL HKHFVNQGTD RNSWHLRKVR
     FVPGGKRQLY GFLANKEDME AFNKHCHGKS RLKYEMRSYN EMVVIQMKQM SEDNQQLNYL
     KNKMVKTEQR SKAVEESLGV VTQKLRETIE ENIFVRSKAK EKHMEYEEEM KSQEEIFHGL
     IEDIHKATED KEKQFEKLLQ EERSKARRFD VDSGTMKDRQ LRKEYVQKFI DCQVKDVAEF
     EVERDELIKV HEDKKLKLKK EYMDLELELE KEFDAALTGL MEKHKPDTFE ASSS