SGT2_SOYBN
ID SGT2_SOYBN Reviewed; 495 AA.
AC D4Q9Z4;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 15-JUN-2010, sequence version 1.
DT 03-AUG-2022, entry version 47.
DE RecName: Full=Soyasapogenol B glucuronide galactosyltransferase;
DE EC=2.4.1.272;
DE AltName: Full=Soyasaponin glycosyltransferase 2;
DE AltName: Full=UDP-galactose:SBMG-galactosyltransferase;
GN Name=GmSGT2;
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP CATALYTIC ACTIVITY.
RX PubMed=20350545; DOI=10.1016/j.febslet.2010.03.037;
RA Shibuya M., Nishimura K., Yasuyama N., Ebizuka Y.;
RT "Identification and characterization of glycosyltransferases involved in
RT the biosynthesis of soyasaponin I in Glycine max.";
RL FEBS Lett. 584:2258-2264(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-260.
RA Cheung F., Xiao Y., Chan A., Moskal W., Town C.D.;
RL Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Glycosyltransferase that transfers a galactosyl group from
CC UDP-galactose to soyasapogenol B monoglucuronide in the biosynthetic
CC pathway for soyasaponins. {ECO:0000269|PubMed:20350545}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=soyasapogenol B 3-O-beta-D-glucuronate + UDP-alpha-D-galactose
CC = H(+) + soyasaponin III + UDP; Xref=Rhea:RHEA:31487,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:62441,
CC ChEBI:CHEBI:62911, ChEBI:CHEBI:66914; EC=2.4.1.272;
CC Evidence={ECO:0000269|PubMed:20350545};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=12 uM for soyasapogenol B 3-O-beta-D-glucuronide
CC {ECO:0000269|PubMed:20350545};
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AB473730; BAI99584.1; -; mRNA.
DR RefSeq; NP_001304384.1; NM_001317455.1.
DR AlphaFoldDB; D4Q9Z4; -.
DR SMR; D4Q9Z4; -.
DR STRING; 3847.GLYMA11G05680.2; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR PRIDE; D4Q9Z4; -.
DR GeneID; 100777250; -.
DR KEGG; ag:BAI99584; -.
DR KEGG; gmx:100777250; -.
DR eggNOG; KOG1192; Eukaryota.
DR InParanoid; D4Q9Z4; -.
DR BioCyc; MetaCyc:MON-16512; -.
DR BRENDA; 2.4.1.272; 2483.
DR Proteomes; UP000008827; Unplaced.
DR GO; GO:0102240; F:soyasapogenol B glucuronide galactosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..495
FT /note="Soyasapogenol B glucuronide galactosyltransferase"
FT /id="PRO_0000418728"
SQ SEQUENCE 495 AA; 55866 MW; 6A6D8E90489829E8 CRC64;
MEKKKGELKS IFLPFLSTSH IIPLVDMARL FALHDVDVTI ITTAHNATVF QKSIDLDASR
GRPIRTHVVN FPAAQVGLPV GIEAFNVDTP REMTPRIYMG LSLLQQVFEK LFHDLQPDFI
VTDMFHPWSV DAAAKLGIPR IMFHGASYLA RSAAHSVEQY APHLEAKFDT DKFVLPGLPD
NLEMTRLQLP DWLRSPNQYT ELMRTIKQSE KKSYGSLFNS FYDLESAYYE HYKSIMGTKS
WGIGPVSLWA NQDAQDKAAR GYAKEEEEKE GWLKWLNSKA ESSVLYVSFG SINKFPYSQL
VEIARALEDS GHDFIWVVRK NDGGEGDNFL EEFEKRMKES NKGYLIWGWA PQLLILENPA
IGGLVTHCGW NTVVESVNAG LPMATWPLFA EHFFNEKLVV DVLKIGVPVG AKEWRNWNEF
GSEVVKREEI GNAIASLMSE EEEDGGMRKR AKELSVAAKS AIKVGGSSHN NMKELIRELK
EIKLSKEAQE TAPNP
