SH21C_MOUSE
ID SH21C_MOUSE Reviewed; 132 AA.
AC Q45HK4; Q45GR3;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=SH2 domain-containing protein 1B2;
DE AltName: Full=EAT-2-related transducer {ECO:0000303|PubMed:16127454};
DE Short=ERT {ECO:0000303|PubMed:16127454};
DE AltName: Full=EAT-2B {ECO:0000303|PubMed:16425036};
GN Name=Sh2d1b2; Synonyms=Eat2b, Sh2d1c;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND INTERACTION
RP WITH CD244.
RC STRAIN=129/Sv;
RX PubMed=16127454; DOI=10.1038/ni1242;
RA Roncagalli R., Taylor J.E., Zhang S., Shi X., Chen R., Cruz-Munoz M.E.,
RA Yin L., Latour S., Veillette A.;
RT "Negative regulation of natural killer cell function by EAT-2, a SAP-
RT related adaptor.";
RL Nat. Immunol. 6:1002-1010(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, AND
RP INTERACTION WITH SLAMF1; HCK; LYN; FYN; FGR AND LCK.
RC STRAIN=129/Sv, and C57BL/6J;
RX PubMed=16425036; DOI=10.1007/s00251-005-0056-3;
RA Calpe S., Erdos E., Liao G., Wang N., Rietdijk S., Simarro M., Scholtz B.,
RA Mooney J., Lee C.H., Shin M.S., Rajnavoelgyi E., Schatzle J.,
RA Morse H.C. III, Terhorst C., Lanyi A.;
RT "Identification and characterization of two related murine genes, Eat2a and
RT Eat2b, encoding single SH2-domain adapters.";
RL Immunogenetics 58:15-25(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP FUNCTION.
RX PubMed=20962259; DOI=10.4049/jimmunol.1001974;
RA Wang N., Calpe S., Westcott J., Castro W., Ma C., Engel P., Schatzle J.D.,
RA Terhorst C.;
RT "The adapters EAT-2A and -2B are positive regulators of CD244- and CD84-
RT dependent NK cell functions in the C57BL/6 mouse.";
RL J. Immunol. 185:5683-5687(2010).
CC -!- FUNCTION: Cytoplasmic adapter regulating receptors of the signaling
CC lymphocytic activation molecule (SLAM) family. In SLAM signaling may
CC cooperate with Sh2d1a/SAP. Plays a role in regulation of effector
CC functions of natural killer (NK) cells by controlling signal
CC transduction through Cd244/2b4. However, conflicting results are
CC reported which may reflect the use of different strain backgrounds.
CC Proposed to act as an inhibitor of Cd244-mediated NK cell function
CC including cytotoxicity and IFN-gamma production, the latter found also
CC by triggering Klra4 and Klrk1 next to Cd244 (PubMed:16127454). Seems to
CC positively regulate Cd244- and Cd84-dependent NK cell functions
CC implicating Cd244-mediated phosphorylation of Vav1 (PubMed:20962259).
CC {ECO:0000269|PubMed:16127454, ECO:0000269|PubMed:20962259}.
CC -!- SUBUNIT: Interacts with SLAMF1 (phosphorylated). Interacts with CD244.
CC Interacts with Src kinases HCK, LYN, FYN, FGR and LCK (via kinase
CC domains). {ECO:0000269|PubMed:16127454, ECO:0000269|PubMed:16425036}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in spleen. Expressed in macrophages,
CC CD8(+) T-Cells and NK cells (PubMed:16425036). Conflictingly found only
CC in NK cells (PubMed:16127454). {ECO:0000269|PubMed:16127454,
CC ECO:0000269|PubMed:16425036}.
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DR EMBL; DQ140178; AAZ94715.1; -; mRNA.
DR EMBL; DQ131181; AAZ66860.1; -; mRNA.
DR EMBL; AC123650; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DQ132512; AAZ66330.1; -; Genomic_DNA.
DR EMBL; DQ132511; AAZ66330.1; JOINED; Genomic_DNA.
DR CCDS; CCDS15469.1; -.
DR RefSeq; NP_001028671.1; NM_001033499.1.
DR AlphaFoldDB; Q45HK4; -.
DR SMR; Q45HK4; -.
DR STRING; 10090.ENSMUSP00000125121; -.
DR PhosphoSitePlus; Q45HK4; -.
DR PaxDb; Q45HK4; -.
DR PRIDE; Q45HK4; -.
DR ProteomicsDB; 261019; -.
DR DNASU; 545378; -.
DR Ensembl; ENSMUST00000162752; ENSMUSP00000125121; ENSMUSG00000073494.
DR GeneID; 545378; -.
DR KEGG; mmu:545378; -.
DR UCSC; uc007dmb.1; mouse.
DR CTD; 545378; -.
DR MGI; MGI:3622649; Sh2d1b2.
DR VEuPathDB; HostDB:ENSMUSG00000073494; -.
DR eggNOG; KOG0565; Eukaryota.
DR GeneTree; ENSGT00940000163577; -.
DR HOGENOM; CLU_125532_0_0_1; -.
DR InParanoid; Q45HK4; -.
DR OMA; IQAAMCL; -.
DR OrthoDB; 1417830at2759; -.
DR PhylomeDB; Q45HK4; -.
DR TreeFam; TF343096; -.
DR Reactome; R-MMU-198933; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
DR BioGRID-ORCS; 545378; 2 hits in 71 CRISPR screens.
DR PRO; PR:Q45HK4; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q45HK4; protein.
DR Bgee; ENSMUSG00000073494; Expressed in ectoplacental cone and 10 other tissues.
DR ExpressionAtlas; Q45HK4; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW.
DR GO; GO:0042267; P:natural killer cell mediated cytotoxicity; IMP:MGI.
DR GO; GO:0045953; P:negative regulation of natural killer cell mediated cytotoxicity; IMP:MGI.
DR GO; GO:0018108; P:peptidyl-tyrosine phosphorylation; IDA:MGI.
DR GO; GO:0045954; P:positive regulation of natural killer cell mediated cytotoxicity; IGI:MGI.
DR GO; GO:0002717; P:positive regulation of natural killer cell mediated immunity; IBA:GO_Central.
DR GO; GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IDA:MGI.
DR GO; GO:0050776; P:regulation of immune response; IBA:GO_Central.
DR GO; GO:0032814; P:regulation of natural killer cell activation; IGI:MGI.
DR GO; GO:0009966; P:regulation of signal transduction; IBA:GO_Central.
DR CDD; cd10342; SH2_SAP1; 1.
DR Gene3D; 3.30.505.10; -; 1.
DR InterPro; IPR035049; EAT2_SH2.
DR InterPro; IPR000980; SH2.
DR InterPro; IPR036860; SH2_dom_sf.
DR Pfam; PF00017; SH2; 1.
DR PRINTS; PR00401; SH2DOMAIN.
DR SMART; SM00252; SH2; 1.
DR SUPFAM; SSF55550; SSF55550; 1.
DR PROSITE; PS50001; SH2; 1.
PE 1: Evidence at protein level;
KW Adaptive immunity; Cytoplasm; Immunity; Innate immunity;
KW Reference proteome; SH2 domain.
FT CHAIN 1..132
FT /note="SH2 domain-containing protein 1B2"
FT /id="PRO_0000435288"
FT DOMAIN 5..101
FT /note="SH2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00191"
FT VARIANT 23
FT /note="G -> S (in strain: 129Sv)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 132 AA; 15044 MW; 23174230605B9400 CRC64;
MDLPYYHGCL TKRECEALLL KGGVDGNFLI RDSESVPGAL CLCVSFKKLV YNYRIFREKN
GYYRIETEPS TPKTIFPNLE ELISKFKTPG QGMVVHLSNP IMRSGFCPGA RRLNLEANVY
ENTDEEYVDV LP
