SH2B3_RAT
ID SH2B3_RAT Reviewed; 368 AA.
AC P50745;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 25-MAY-2022, entry version 121.
DE RecName: Full=SH2B adapter protein 3;
DE AltName: Full=Lymphocyte adapter protein;
DE AltName: Full=Lymphocyte-specific adapter protein Lnk;
DE AltName: Full=Signal transduction protein Lnk;
GN Name=Sh2b3; Synonyms=Lnk;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LNK1; LNK2; LNK3 AND LNK4).
RC STRAIN=Fischer; TISSUE=Lymph node;
RX PubMed=8524815; DOI=10.1073/pnas.92.25.11618;
RA Huang X., Li Y., Tanaka K., Moore K.G., Hayashi J.;
RT "Cloning and characterization of Lnk, a signal transduction protein that
RT links T-cell receptor activation signal to phospholipase C gamma 1, Grb2,
RT and phosphatidylinositol 3-kinase.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:11618-11622(1995).
CC -!- FUNCTION: Links T-cell receptor activation signal to phospholipase C-
CC gamma-1, GRB2 and phosphatidylinositol 3-kinase.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=Lnk4;
CC IsoId=P50745-1; Sequence=Displayed;
CC Name=Lnk1;
CC IsoId=P50745-2; Sequence=VSP_004310;
CC Name=Lnk2;
CC IsoId=P50745-3; Sequence=VSP_004308, VSP_004311;
CC Name=Lnk3;
CC IsoId=P50745-4; Sequence=VSP_004309, VSP_004311;
CC -!- TISSUE SPECIFICITY: Preferentially expressed by lymphocytes in lymph
CC node and spleen.
CC -!- PTM: Tyrosine-phosphorylated upon T-cell activation. The tyrosine
CC phosphorylation site is multifunctional and may associate with the SH2
CC domains of phospholipase C-gamma-1, GRB-2, and PI3-K upon T-cell
CC receptor activation.
CC -!- SIMILARITY: Belongs to the SH2B adapter family. {ECO:0000305}.
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DR EMBL; U24655; AAC52351.1; -; mRNA.
DR EMBL; U24654; AAC52350.1; -; mRNA.
DR EMBL; U24653; AAC52349.1; -; mRNA.
DR EMBL; U24652; AAC52348.1; -; mRNA.
DR RefSeq; NP_113809.1; NM_031621.1. [P50745-1]
DR AlphaFoldDB; P50745; -.
DR SMR; P50745; -.
DR BioGRID; 248640; 2.
DR STRING; 10116.ENSRNOP00000066850; -.
DR PaxDb; P50745; -.
DR PRIDE; P50745; -.
DR GeneID; 58838; -.
DR KEGG; rno:58838; -.
DR UCSC; RGD:68411; rat. [P50745-1]
DR CTD; 10019; -.
DR RGD; 68411; Sh2b3.
DR eggNOG; ENOG502QS89; Eukaryota.
DR HOGENOM; CLU_014885_3_0_1; -.
DR InParanoid; P50745; -.
DR OrthoDB; 556279at2759; -.
DR PhylomeDB; P50745; -.
DR PRO; PR:P50745; -.
DR Proteomes; UP000002494; Unplaced.
DR Genevisible; P50745; RN.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0042301; F:phosphate ion binding; IDA:RGD.
DR GO; GO:1990782; F:protein tyrosine kinase binding; ISO:RGD.
DR GO; GO:0044877; F:protein-containing complex binding; IDA:RGD.
DR GO; GO:0030159; F:signaling receptor complex adaptor activity; ISO:RGD.
DR GO; GO:0005173; F:stem cell factor receptor binding; ISO:RGD.
DR GO; GO:0005068; F:transmembrane receptor protein tyrosine kinase adaptor activity; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; ISO:RGD.
DR GO; GO:1990869; P:cellular response to chemokine; ISO:RGD.
DR GO; GO:0036016; P:cellular response to interleukin-3; ISO:RGD.
DR GO; GO:0035162; P:embryonic hemopoiesis; ISO:RGD.
DR GO; GO:0048821; P:erythrocyte development; ISO:RGD.
DR GO; GO:0030097; P:hemopoiesis; ISO:RGD.
DR GO; GO:0035556; P:intracellular signal transduction; ISO:RGD.
DR GO; GO:0035855; P:megakaryocyte development; ISO:RGD.
DR GO; GO:0035702; P:monocyte homeostasis; ISO:RGD.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISO:RGD.
DR GO; GO:0070100; P:negative regulation of chemokine-mediated signaling pathway; ISO:RGD.
DR GO; GO:1900235; P:negative regulation of Kit signaling pathway; ISO:RGD.
DR GO; GO:0043407; P:negative regulation of MAP kinase activity; ISO:RGD.
DR GO; GO:0090331; P:negative regulation of platelet aggregation; ISO:RGD.
DR GO; GO:0051898; P:negative regulation of protein kinase B signaling; ISO:RGD.
DR GO; GO:0046426; P:negative regulation of receptor signaling pathway via JAK-STAT; ISO:RGD.
DR GO; GO:0060761; P:negative regulation of response to cytokine stimulus; ISO:RGD.
DR GO; GO:1903671; P:negative regulation of sprouting angiogenesis; IMP:RGD.
DR GO; GO:0042532; P:negative regulation of tyrosine phosphorylation of STAT protein; ISO:RGD.
DR GO; GO:0001780; P:neutrophil homeostasis; ISO:RGD.
DR GO; GO:0045589; P:regulation of regulatory T cell differentiation; IMP:RGD.
DR GO; GO:0038163; P:thrombopoietin-mediated signaling pathway; ISO:RGD.
DR CDD; cd10412; SH2_SH2B3; 1.
DR Gene3D; 3.30.505.10; -; 1.
DR InterPro; IPR000980; SH2.
DR InterPro; IPR036860; SH2_dom_sf.
DR InterPro; IPR030523; SH2B.
DR InterPro; IPR030522; SH2B3.
DR InterPro; IPR035059; SH2B3_SH2.
DR PANTHER; PTHR10872; PTHR10872; 1.
DR PANTHER; PTHR10872:SF1; PTHR10872:SF1; 1.
DR Pfam; PF00017; SH2; 1.
DR PRINTS; PR00401; SH2DOMAIN.
DR SMART; SM00252; SH2; 1.
DR SUPFAM; SSF55550; SSF55550; 1.
DR PROSITE; PS50001; SH2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Phosphoprotein; Reference proteome; SH2 domain.
FT CHAIN 1..368
FT /note="SH2B adapter protein 3"
FT /id="PRO_0000084456"
FT DOMAIN 119..217
FT /note="SH2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00191"
FT MOD_RES 356
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..95
FT /note="MAGRTQGKHRPRVRTCPSLVPFDNPCWPPSHQPLPLQVGAPGHRVTSFLSGR
FT AWPSYIPKGKHGLPGPRYVPDPWSLGVLGHVAPLRVTTTIFLP -> MPDNLYTFVLKV
FT QGQTDIIFEVGDEQQLNSWLAELRASTGLGLEHLDTELPLSLVAEPGPAISPRGSTDSL
FT DQ (in isoform Lnk2)"
FT /evidence="ECO:0000303|PubMed:8524815"
FT /id="VSP_004308"
FT VAR_SEQ 14..38
FT /note="Missing (in isoform Lnk3)"
FT /evidence="ECO:0000303|PubMed:8524815"
FT /id="VSP_004309"
FT VAR_SEQ 225..278
FT /note="Missing (in isoform Lnk1)"
FT /evidence="ECO:0000303|PubMed:8524815"
FT /id="VSP_004310"
FT VAR_SEQ 226..262
FT /note="Missing (in isoform Lnk2 and isoform Lnk3)"
FT /evidence="ECO:0000303|PubMed:8524815"
FT /id="VSP_004311"
SQ SEQUENCE 368 AA; 40432 MW; 624CC2ABA0332C87 CRC64;
MAGRTQGKHR PRVRTCPSLV PFDNPCWPPS HQPLPLQVGA PGHRVTSFLS GRAWPSYIPK
GKHGLPGPRY VPDPWSLGVL GHVAPLRVTT TIFLPGASPG VMLDPACQKT DHFLSCYPWF
HGPISRVRAA QLVQLQGPDA HGVFLVRQSE SRRGEYVLTF NLQGRAKHLR LVLTERGQCR
VQHLHFPSVV DMLRHFQRSP IPLECGAACD VRLSGYVVVV SQAPGRKPKV VSRGGVTAST
ARTKLPSWLR LLHGVIHVIS PGSSNTVLFP FSLPHWDSEL GHPHLSSAGC PPGHGAEALR
GQVTPPEQIF HLVPSPEELA NSLRQLELES VSSARDSDYE MDSSSRSHLR AIDNQYTPLS
QLCREANL
