SHAWN_DROME
ID SHAWN_DROME Reviewed; 387 AA.
AC Q9VWG0; Q8SZT6;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Solute carrier family 25 protein Shawn {ECO:0000305};
DE AltName: Full=Solute carrier family 25 member 39 homolog;
GN Name=Shawn {ECO:0000303|PubMed:26865615, ECO:0000312|FlyBase:FBgn0031039};
GN ORFNames=CG14209 {ECO:0000312|FlyBase:FBgn0031039};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF GLY-278.
RX PubMed=26865615; DOI=10.1523/jneurosci.3432-15.2016;
RA Slabbaert J.R., Kuenen S., Swerts J., Maes I., Uytterhoeven V.,
RA Kasprowicz J., Fernandes A.C., Blust R., Verstreken P.;
RT "Shawn, the Drosophila Homolog of SLC25A39/40, Is a Mitochondrial Carrier
RT That Promotes Neuronal Survival.";
RL J. Neurosci. 36:1914-1929(2016).
CC -!- FUNCTION: Mitochondrial transporter required for glutathione import
CC into mitochondria. {ECO:0000250|UniProtKB:Q9BZJ4}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000305|PubMed:26865615}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Lethality caused by neurodegeneration and
CC synaptic defects. {ECO:0000269|PubMed:26865615}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; AE014298; AAF48981.2; -; Genomic_DNA.
DR EMBL; AE014298; AAF48982.2; -; Genomic_DNA.
DR EMBL; AE014298; AAN09511.1; -; Genomic_DNA.
DR EMBL; AE014298; AHN59918.1; -; Genomic_DNA.
DR EMBL; AY070516; AAL47987.1; -; mRNA.
DR EMBL; BT125676; ADN93321.1; -; mRNA.
DR RefSeq; NP_001027071.1; NM_001031900.3.
DR RefSeq; NP_001027072.1; NM_001031901.2.
DR RefSeq; NP_001027073.1; NM_001031902.2.
DR RefSeq; NP_001285448.1; NM_001298519.1.
DR AlphaFoldDB; Q9VWG0; -.
DR SMR; Q9VWG0; -.
DR IntAct; Q9VWG0; 1.
DR STRING; 7227.FBpp0074506; -.
DR PaxDb; Q9VWG0; -.
DR PRIDE; Q9VWG0; -.
DR DNASU; 3772641; -.
DR EnsemblMetazoa; FBtr0074737; FBpp0074506; FBgn0031039.
DR EnsemblMetazoa; FBtr0074738; FBpp0074507; FBgn0031039.
DR EnsemblMetazoa; FBtr0074739; FBpp0074508; FBgn0031039.
DR EnsemblMetazoa; FBtr0344069; FBpp0310500; FBgn0031039.
DR GeneID; 3772641; -.
DR KEGG; dme:Dmel_CG14209; -.
DR UCSC; CG14209-RB; d. melanogaster.
DR CTD; 3772641; -.
DR FlyBase; FBgn0031039; Shawn.
DR VEuPathDB; VectorBase:FBgn0031039; -.
DR eggNOG; KOG0761; Eukaryota.
DR GeneTree; ENSGT00940000167433; -.
DR HOGENOM; CLU_015166_0_0_1; -.
DR OMA; IYWTTYE; -.
DR PhylomeDB; Q9VWG0; -.
DR BioGRID-ORCS; 3772641; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Tyler; fly.
DR GenomeRNAi; 3772641; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0031039; Expressed in testis and 11 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IDA:FlyBase.
DR GO; GO:0022857; F:transmembrane transporter activity; ISM:FlyBase.
DR GO; GO:0055065; P:metal ion homeostasis; IMP:FlyBase.
DR GO; GO:1990542; P:mitochondrial transmembrane transport; ISM:FlyBase.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR InterPro; IPR045315; Mtm1-like.
DR PANTHER; PTHR45760; PTHR45760; 1.
DR Pfam; PF00153; Mito_carr; 3.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..387
FT /note="Solute carrier family 25 protein Shawn"
FT /id="PRO_0000454894"
FT TRANSMEM 43..63
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 235..255
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 275..295
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 337..357
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 37..156
FT /note="Solcar"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 179..263
FT /note="Solcar"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 269..366
FT /note="Solcar"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT MUTAGEN 278
FT /note="G->E: Mutant flies show an accumulation of reactive
FT oxygen species, mitochondrial dysfunction and
FT neurodegeneration."
FT /evidence="ECO:0000269|PubMed:26865615"
SQ SEQUENCE 387 AA; 42167 MW; D3C01956BF270D23 CRC64;
MATREACGQF AAASAAMAAA SSQNPSKATM TDPRFRIRPL QQVASACTGA MVTACFMTPL
DVIKTRLQAQ QQALLSNKCF LYCNGLMDHI CPCGPDTPNP AAAKPAPRFS GTIDAFIKIS
RTEGIGSLWS GLSPTLISAL PSTIIYFVAY EQFKARFTDI HYKYTRRPDT IAHDIPHPIP
FLVPLLAGVS GRILAVTCVS PVELIRTKMQ SQRMTHAEMF GTIRQVVQSQ GVLGLWRGLP
PTILRDVPFS GIYWTCYEYL KSSFGVVEPT FSFSFAAGAI SGSVAATITT PFDVVKTHEQ
IEFGEKFIFS DNPPKQVATK SVAMRLASIY RMGGVPAIFS GLGPRLFKVA PACAIMISSF
EYGKSFFYHY NIDQHNRSNQ ATKGPGS
