ID   SHBA_STRGG              Reviewed;         191 AA.
AC   B1W3T1;
DT   31-JAN-2018, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=ECF RNA polymerase sigma factor ShbA {ECO:0000305};
DE   AltName: Full=Sigma factor for hrdB {ECO:0000303|PubMed:23347076};
GN   Name=shbA {ECO:0000303|PubMed:23347076};
GN   OrderedLocusNames=SGR_2758 {ECO:0000312|EMBL:BAG19587.1};
OS   Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=455632;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 4626 / NBRC 13350;
RX   PubMed=18375553; DOI=10.1128/jb.00204-08;
RA   Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H.,
RA   Yamashita A., Hattori M., Horinouchi S.;
RT   "Genome sequence of the streptomycin-producing microorganism Streptomyces
RT   griseus IFO 13350.";
RL   J. Bacteriol. 190:4050-4060(2008).
RN   [2]
RP   FUNCTION, DNA-BINDING, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=JCM 4626 / NBRC 13350;
RX   PubMed=23347076; DOI=10.1111/mmi.12160;
RA   Otani H., Higo A., Nanamiya H., Horinouchi S., Ohnishi Y.;
RT   "An alternative sigma factor governs the principal sigma factor in
RT   Streptomyces griseus.";
RL   Mol. Microbiol. 87:1223-1236(2013).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. Extracytoplasmic function (ECF) sigma factors are held in an
CC       inactive form by an anti-sigma factor until released (Probable). This
CC       alternative sigma factor governs the transcription of the principal
CC       sigma factor HrdB (SigA) throughout growth. Acts by binding to the
CC       promoter region (PubMed:23347076). {ECO:0000269|PubMed:23347076,
CC       ECO:0000305}.
CC   -!- INDUCTION: Expression is enhanced during the late growth stage.
CC       {ECO:0000269|PubMed:23347076}.
CC   -!- DOMAIN: The sigma-70 factor domain-2 mediates sequence-specific
CC       interaction with the -10 element in promoter DNA, and plays an
CC       important role in melting the double-stranded DNA and the formation of
CC       the transcription bubble. The sigma-70 factor domain-2 mediates
CC       interaction with the RNA polymerase subunits RpoB and RpoC.
CC       {ECO:0000250|UniProtKB:Q9EZJ8}.
CC   -!- DOMAIN: The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-
CC       H) motif that mediates interaction with the -35 element in promoter
CC       DNA. The domain also mediates interaction with the RNA polymerase
CC       subunit RpoA. Interactions between sigma-70 factor domain-4 and anti-
CC       sigma factors prevents interaction of sigma factors with the RNA
CC       polymerase catalytic core. {ECO:0000250|UniProtKB:Q9EZJ8}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant shows a severe growth defect.
CC       Transcription of many genes, including hrdB (sigA), are down-regulated
CC       in the mutant strain. {ECO:0000269|PubMed:23347076}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. ECF subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AP009493; BAG19587.1; -; Genomic_DNA.
DR   RefSeq; WP_003966888.1; NC_010572.1.
DR   AlphaFoldDB; B1W3T1; -.
DR   SMR; B1W3T1; -.
DR   STRING; 455632.SGR_2758; -.
DR   EnsemblBacteria; BAG19587; BAG19587; SGR_2758.
DR   GeneID; 6215410; -.
DR   KEGG; sgr:SGR_2758; -.
DR   PATRIC; fig|455632.4.peg.2820; -.
DR   eggNOG; COG1595; Bacteria.
DR   HOGENOM; CLU_047691_10_1_11; -.
DR   OMA; AVKVGIH; -.
DR   OrthoDB; 1914729at2; -.
DR   Proteomes; UP000001685; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR039425; RNA_pol_sigma-70-like.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR43133; PTHR43133; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Sigma factor; Transcription; Transcription regulation.
FT   CHAIN           1..191
FT                   /note="ECF RNA polymerase sigma factor ShbA"
FT                   /id="PRO_0000442939"
FT   DNA_BIND        160..179
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250|UniProtKB:Q9EZJ8"
FT   REGION          27..98
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255"
FT   REGION          100..122
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          138..187
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   191 AA;  20921 MW;  DDE3F33433CADF54 CRC64;
     MRDDETTVIG ALVHRAVEGD AQATHDLLAH VHPLALRYCR SRLNRLPGDA RHFVEDLAQE
     VCVAVLMALP RYKDTGRPFE AFVFAIAGHK VADLQRAAMR HPGSTAVPSD EMPERPDDSL
     GPEERALLSS DAAWAKKLLA NLPENQRELL VLRVAVGLTA EETGQMLGMS PGAVRVAQHR
     ALSRLRALAE Q