SHB_MOUSE
ID SHB_MOUSE Reviewed; 503 AA.
AC Q6PD21; A2AKW3; Q3ULM3;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=SH2 domain-containing adapter protein B;
GN Name=Shb;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Fetal brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=8302579;
RA Welsh M., Mares J., Karlsson T., Lavergne C., Breant B., Claesson-Welsh L.;
RT "Shb is a ubiquitously expressed Src homology 2 protein.";
RL Oncogene 9:19-27(1994).
RN [5]
RP INDUCTION BY OKADAIC ACID AND GENISTEIN.
RX PubMed=8777141; DOI=10.1016/0898-6568(95)02019-5;
RA Lavergne C., Mares J., Karlsson T., Breant B., Welsh M.;
RT "Control of SHB gene expression by protein phosphorylation.";
RL Cell. Signal. 8:55-58(1996).
RN [6]
RP INTERACTION WITH PTPN11.
RX PubMed=12181353; DOI=10.1091/mbc.e02-02-0103;
RA Cross M.J., Lu L., Magnusson P., Nyqvist D., Holmqvist K., Welsh M.,
RA Claesson-Welsh L.;
RT "The Shb adaptor protein binds to tyrosine 766 in the FGFR-1 and regulates
RT the Ras/MEK/MAPK pathway via FRS2 phosphorylation in endothelial cells.";
RL Mol. Biol. Cell 13:2881-2893(2002).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-382, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-382, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Kidney, Lung, and Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Adapter protein which regulates several signal transduction
CC cascades by linking activated receptors to downstream signaling
CC components. May play a role in angiogenesis by regulating FGFR1, VEGFR2
CC and PDGFR signaling. May also play a role in T-cell antigen
CC receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal
CC cells differentiation by mediating basic-FGF and NGF-induced signaling
CC cascades. May also regulate IRS1 and IRS2 signaling in insulin-
CC producing cells (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with phosphorylated 'Tyr-720' of the ligand-
CC activated receptor PDGFRA via its SH2 domain. Interacts with the
CC ligand-activated receptors PDGFRB, FGFR1, KDR/VEGFR2, IL2RB and IL2RG.
CC Interacts with EPS8 and V-SRC. Interacts with GRB2 and GRAP. Interacts
CC with CD3Z. Interacts with tyrosine-phosphorylated LAT upon T-cell
CC antigen receptor activation. Interacts with PLCG1. Interacts with
CC ZAP70, LCP2/SLP-76, VAV1 and GRAP2. Interacts with JAK1 and JAK3.
CC Interacts with PTK2/FAK1. Interacts with CRK/CrKII. Interacts with IRS2
CC (By similarity). Interacts with PTPN11. {ECO:0000250,
CC ECO:0000269|PubMed:12181353}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Cell membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Note=Associates with membrane lipid rafts upon TCR
CC stimulation. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6PD21-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6PD21-2; Sequence=VSP_019848, VSP_019849, VSP_019850;
CC -!- TISSUE SPECIFICITY: Expressed in heart, liver, brain and kidney (at
CC protein level). {ECO:0000269|PubMed:8302579}.
CC -!- INDUCTION: Up-regulated by okadaic acid and genistein.
CC {ECO:0000269|PubMed:8777141}.
CC -!- DOMAIN: The SH2 domain preferentially binds phosphopeptides with the
CC consensus sequence Y-[TVI]-X-L and mediates interaction with PDGFRA,
CC PDGFRB, FGRFR1, IL2RB, IL2RG, CD3Z and CRK/CrKII. {ECO:0000250}.
CC -!- PTM: Phosphorylated upon PDGFRA, PDGFRB, TCR, IL2 receptor, FGFR1 or
CC VEGFR2 activation. {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH58986.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK145414; BAE26425.1; -; mRNA.
DR EMBL; AL772376; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC058986; AAH58986.1; ALT_INIT; mRNA.
DR CCDS; CCDS51173.1; -. [Q6PD21-1]
DR RefSeq; NP_001028478.1; NM_001033306.1. [Q6PD21-1]
DR AlphaFoldDB; Q6PD21; -.
DR SMR; Q6PD21; -.
DR BioGRID; 230937; 2.
DR IntAct; Q6PD21; 2.
DR MINT; Q6PD21; -.
DR STRING; 10090.ENSMUSP00000060433; -.
DR iPTMnet; Q6PD21; -.
DR PhosphoSitePlus; Q6PD21; -.
DR MaxQB; Q6PD21; -.
DR PaxDb; Q6PD21; -.
DR PRIDE; Q6PD21; -.
DR ProteomicsDB; 261349; -. [Q6PD21-1]
DR ProteomicsDB; 261350; -. [Q6PD21-2]
DR Ensembl; ENSMUST00000061986; ENSMUSP00000060433; ENSMUSG00000044813. [Q6PD21-1]
DR GeneID; 230126; -.
DR KEGG; mmu:230126; -.
DR UCSC; uc008sst.2; mouse. [Q6PD21-1]
DR UCSC; uc008ssu.1; mouse. [Q6PD21-2]
DR CTD; 6461; -.
DR MGI; MGI:98294; Shb.
DR VEuPathDB; HostDB:ENSMUSG00000044813; -.
DR eggNOG; ENOG502RT81; Eukaryota.
DR GeneTree; ENSGT00940000161591; -.
DR HOGENOM; CLU_029444_0_0_1; -.
DR InParanoid; Q6PD21; -.
DR OMA; QFNGGEK; -.
DR OrthoDB; 1120795at2759; -.
DR PhylomeDB; Q6PD21; -.
DR TreeFam; TF325799; -.
DR Reactome; R-MMU-4420097; VEGFA-VEGFR2 Pathway.
DR BioGRID-ORCS; 230126; 5 hits in 73 CRISPR screens.
DR ChiTaRS; Shb; mouse.
DR PRO; PR:Q6PD21; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q6PD21; protein.
DR Bgee; ENSMUSG00000044813; Expressed in gastrula and 199 other tissues.
DR ExpressionAtlas; Q6PD21; baseline and differential.
DR Genevisible; Q6PD21; MM.
DR GO; GO:0036464; C:cytoplasmic ribonucleoprotein granule; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0001784; F:phosphotyrosine residue binding; ISO:MGI.
DR GO; GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0042100; P:B cell proliferation; IDA:MGI.
DR GO; GO:0001568; P:blood vessel development; IMP:MGI.
DR GO; GO:0048514; P:blood vessel morphogenesis; IMP:MGI.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0071425; P:hematopoietic stem cell proliferation; IMP:MGI.
DR GO; GO:0030097; P:hemopoiesis; IMP:MGI.
DR GO; GO:1900194; P:negative regulation of oocyte maturation; IMP:MGI.
DR GO; GO:0006469; P:negative regulation of protein kinase activity; IMP:MGI.
DR GO; GO:0045931; P:positive regulation of mitotic cell cycle; IMP:MGI.
DR GO; GO:0045624; P:positive regulation of T-helper cell differentiation; IMP:MGI.
DR GO; GO:0050852; P:T cell receptor signaling pathway; IMP:MGI.
DR CDD; cd10389; SH2_SHB; 1.
DR Gene3D; 3.30.505.10; -; 1.
DR InterPro; IPR000980; SH2.
DR InterPro; IPR036860; SH2_dom_sf.
DR InterPro; IPR035040; SHB.
DR InterPro; IPR035045; SHB_SH2.
DR PANTHER; PTHR15127:SF31; PTHR15127:SF31; 1.
DR Pfam; PF00017; SH2; 1.
DR PRINTS; PR00401; SH2DOMAIN.
DR SMART; SM00252; SH2; 1.
DR SUPFAM; SSF55550; SSF55550; 1.
DR PROSITE; PS50001; SH2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Angiogenesis; Apoptosis; Cell membrane; Cytoplasm;
KW Developmental protein; Differentiation; Isopeptide bond; Membrane;
KW Phosphoprotein; Reference proteome; SH2 domain; Ubl conjugation.
FT CHAIN 1..503
FT /note="SH2 domain-containing adapter protein B"
FT /id="PRO_0000246325"
FT DOMAIN 404..498
FT /note="SH2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00191"
FT REGION 1..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 61..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 147..180
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 225..262
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 292..333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 345..381
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..39
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 61..75
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 147..170
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 231..254
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 309..332
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 101
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q15464"
FT MOD_RES 301
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q15464"
FT MOD_RES 311
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q15464"
FT MOD_RES 382
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319,
FT ECO:0007744|PubMed:21183079"
FT CROSSLNK 186
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q15464"
FT VAR_SEQ 1..262
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_019848"
FT VAR_SEQ 443..479
FT /note="KSNQGFMHMKLAKTKEKYVLGQNSPPFDSVPEVIHYY -> NYADPEAVCAM
FT PILPRTARPSVRPSVHPSVRKICARR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_019849"
FT VAR_SEQ 480..503
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_019850"
SQ SEQUENCE 503 AA; 54708 MW; 9A668DFC429F41E3 CRC64;
MAKWLNKYFS LGNSKTKSPP QPPRPDYREQ RRRGERREQP PQAVPQACSA SSASCGSAAA
CFSASSGSLP DDSGSTSDLI RAYRAQKERD FEDPYNGPGS SLRKLRAMCR LDYCGGGGGG
DPGGGQRAFT AAAGAAGCCC AAAGAGAAAS SSSSSGSPHL YRSSSERRPT TPAEVRYISP
KHRLIKVESA SAAGDPPGGV CSGGRTWSPT TCGGKKLLNK CSAEETGAGQ KDKVTIADDY
SDPFDAKSDL KSKAGKGESA GYMEPYEAQR IMTEFQRQES VRSQHKGIQL YDTPYEPEGQ
SVDSDSESTV SLRLRESKLP QDDDRPADEY DQPWEWNRVT IPALAAQFNG NEKRQSSPSP
SRDRRRQLRA PGGGFKPIKH GSPEFCGILG ERVDPTIPLE KQIWYHGAIS RSDAENLLRL
CKECSYLVRN SQTSKHDYSL SLKSNQGFMH MKLAKTKEKY VLGQNSPPFD SVPEVIHYYT
TRKLPIKGAE HLSLLYPVAV RTL
