SHC1_SCHJY
ID SHC1_SCHJY Reviewed; 656 AA.
AC B6K412;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 1.
DT 03-AUG-2022, entry version 60.
DE RecName: Full=Squalene-hopene cyclase {ECO:0000303|PubMed:34353908};
DE Short=SHC {ECO:0000303|PubMed:34353908};
DE EC=4.2.1.129 {ECO:0000305|PubMed:34353908};
DE EC=5.4.99.17 {ECO:0000305|PubMed:34353908};
GN Name=shc1 {ECO:0000303|PubMed:34353908}; ORFNames=SJAG_03360;
OS Schizosaccharomyces japonicus (strain yFS275 / FY16936) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=402676;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=yFS275 / FY16936;
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [2]
RP FUNCTION, AND BIOTECHNOLOGY.
RX PubMed=34353908; DOI=10.1073/pnas.2105225118;
RA Bouwknegt J., Wiersma S.J., Ortiz-Merino R.A., Doornenbal E.S.R.,
RA Buitenhuis P., Giera M., Mueller C., Pronk J.T.;
RT "A squalene-hopene cyclase in Schizosaccharomyces japonicus represents a
RT eukaryotic adaptation to sterol-limited anaerobic environments.";
RL Proc. Natl. Acad. Sci. U.S.A. 118:0-0(2021).
CC -!- FUNCTION: Squalene cyclase that catalyzes the oxygen-independent
CC cyclization of squalene into hopanoids, a class of cyclic triterpenoids
CC including hop-22(29)-ene, hop-17(21)-ene, hop-21(22)-ene, and hopan-22-
CC ol. {ECO:0000269|PubMed:34353908}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=squalene = hop-22(29)-ene; Xref=Rhea:RHEA:17637,
CC ChEBI:CHEBI:4648, ChEBI:CHEBI:15440; EC=5.4.99.17;
CC Evidence={ECO:0000305|PubMed:34353908};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17638;
CC Evidence={ECO:0000305|PubMed:34353908};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + squalene = hopan-22-ol; Xref=Rhea:RHEA:16561,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15440, ChEBI:CHEBI:36484;
CC EC=4.2.1.129; Evidence={ECO:0000305|PubMed:34353908};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16562;
CC Evidence={ECO:0000305|PubMed:34353908};
CC -!- BIOTECHNOLOGY: The fast anaerobic growth of Schizosaccharomyces
CC japonicus in sterol-free media is an interesting trait for developing
CC robust fungal cell factories for application in anaerobic industrial
CC processes. {ECO:0000269|PubMed:34353908}.
CC -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC {ECO:0000305}.
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DR EMBL; KE651167; EEB08219.1; -; Genomic_DNA.
DR RefSeq; XP_002174512.1; XM_002174476.2.
DR STRING; 4897.EEB08219; -.
DR EnsemblFungi; EEB08219; EEB08219; SJAG_03360.
DR GeneID; 7050081; -.
DR VEuPathDB; FungiDB:SJAG_03360; -.
DR eggNOG; KOG0497; Eukaryota.
DR HOGENOM; CLU_019345_0_0_1; -.
DR OMA; DYYRHYF; -.
DR OrthoDB; 365003at2759; -.
DR Proteomes; UP000001744; Unassembled WGS sequence.
DR GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0051007; F:squalene-hopene cyclase activity; IEA:RHEA.
DR GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR CDD; cd02892; SQCY_1; 1.
DR InterPro; IPR006400; Hopene-cyclase.
DR InterPro; IPR032696; SQ_cyclase_C.
DR InterPro; IPR032697; SQ_cyclase_N.
DR InterPro; IPR018333; Squalene_cyclase.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR PANTHER; PTHR11764; PTHR11764; 1.
DR Pfam; PF13243; SQHop_cyclase_C; 1.
DR Pfam; PF13249; SQHop_cyclase_N; 1.
DR SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR TIGRFAMs; TIGR01507; hopene_cyclase; 1.
DR TIGRFAMs; TIGR01787; squalene_cyclas; 1.
PE 1: Evidence at protein level;
KW Isomerase; Lyase; Reference proteome; Repeat.
FT CHAIN 1..656
FT /note="Squalene-hopene cyclase"
FT /id="PRO_0000455302"
FT REPEAT 68..110
FT /note="PFTB 1"
FT /evidence="ECO:0000255"
FT REPEAT 417..459
FT /note="PFTB 2"
FT /evidence="ECO:0000255"
FT REPEAT 485..525
FT /note="PFTB 3"
FT /evidence="ECO:0000255"
FT REPEAT 533..582
FT /note="PFTB 4"
FT /evidence="ECO:0000255"
FT REPEAT 591..634
FT /note="PFTB 5"
FT /evidence="ECO:0000255"
FT ACT_SITE 396
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:P48449"
FT SITE 324
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250|UniProtKB:P48449"
FT SITE 506
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250|UniProtKB:P48449"
SQ SEQUENCE 656 AA; 74242 MW; 0A9E4F20813CCD0A CRC64;
MKTDGNTTLD TTISMEELER TVKSAYEALA KDQQDDGHWI YELEADVTIP AQFILLEHTL
DKIDEELEQK IANYLRRCQS REHWGWPVYY GGEFNISASV QAYFALKMTG EDINAPHMVR
AREAILAHGG PEYANVFTRI QLSLFGEASW LATPFMPVEI MLLPRWMYFS IWNMSYWSRT
TVAPLLIVAD LKPKAINPRN VHIPELFPTP PDKVKTWIHG PFRSKWGHVF KFIDTAIRPF
TRFVPSFLHK KAYKAALDFI EPRLNGVDGL GAIYPPMSYS AVMYRALGIP DDDPRAATNW
EALKGLLVIK EREAYCQACV SPVWDTALSG HALMEASFGP DGINADRTEK LIDRAAHWLR
AHQVLNVVGD WAINNPNLQP GGWAFQYGND YYPDVDDTAV AAMLLHRQNL PENEEALDRA
RKWIIGMQSS NGGWGAFDID NDKQILNDIP FADHGALLDP PTADVSARCI SLLAELGHPE
DRPVIERGIK YLRKEQEEDG SWFGRWGTNY IYGAWSVLCA FNASGVPHDD PSVLKCVNFL
KSVQREDGGW GESCETYEGS AHGVYTESLP SQTAWAVLGL MASGRRTDPA VKRGIVWLIQ
HQQDNGEWAE EPFNAVGFPR MFYLHYLGYK QFFPLLALAR YRHMEKSGTN NVSFAF
