SHCAF_MOUSE
ID SHCAF_MOUSE Reviewed; 221 AA.
AC Q8C8M1; P70361; Q3TLD2;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=SIN3-HDAC complex-associated factor;
DE AltName: Full=Protein FAM60A;
DE AltName: Full=Tera protein {ECO:0000303|Ref.1};
GN Name=Sinhcaf; Synonyms=Fam60a, Tera {ECO:0000303|Ref.1};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Teratocarcinoma;
RA Malnar-Dragojevic D., Trachtulec Z., Vincek V.;
RT "A new mouse gene with restricted expression pattern.";
RL Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Adrenal gland;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION, IDENTIFICATION IN A COMPLEX WITH SIN3A; HDAC1; SAP30; RBBP4; OGT
RP AND TET1, INTERACTION WITH SIN3A AND OGT, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=28554894; DOI=10.15252/embj.201696307;
RA Streubel G., Fitzpatrick D.J., Oliviero G., Scelfo A., Moran B., Das S.,
RA Munawar N., Watson A., Wynne K., Negri G.L., Dillon E.T., Jammula S.,
RA Hokamp K., O'Connor D.P., Pasini D., Cagney G., Bracken A.P.;
RT "Fam60a defines a variant Sin3a-Hdac complex in embryonic stem cells
RT required for self-renewal.";
RL EMBO J. 36:2216-2232(2017).
CC -!- FUNCTION: Subunit of the Sin3 deacetylase complex (Sin3/HDAC), this
CC subunit is important for the repression of genes encoding components of
CC the TGF-beta signaling pathway (By similarity). Core component of a
CC SIN3A complex (composed of at least SINHCAF, SIN3A, HDAC1, SAP30,
CC RBBP4, OGT and TET1) present in embryonic stem (ES) cells. Promotes the
CC stability of SIN3A and its presence on chromatin and is essential for
CC maintaining the potential of ES cells to proliferate rapidly, while
CC ensuring a short G1-phase of the cell cycle, thereby preventing
CC premature lineage priming (PubMed:28554894).
CC {ECO:0000250|UniProtKB:Q9NP50, ECO:0000269|PubMed:28554894}.
CC -!- SUBUNIT: Component of the Sin3/HDAC corepressor complex at least
CC composed of BRMS1, BRMS1L, ING2, SAP30, SAP30L, HDAC1 (By similarity).
CC Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30,
CC RBBP4, OGT and TET1. Interacts with SIN3A and OGT (PubMed:28554894).
CC {ECO:0000250|UniProtKB:Q9NP50, ECO:0000269|PubMed:28554894}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:28554894}.
CC -!- TISSUE SPECIFICITY: Embryonic stem cells (at protein level).
CC {ECO:0000269|PubMed:28554894}.
CC -!- SIMILARITY: Belongs to the SINHCAF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB09547.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U64033; AAB09547.1; ALT_INIT; mRNA.
DR EMBL; AK046519; BAC32768.1; -; mRNA.
DR EMBL; AK145384; BAE26402.1; -; mRNA.
DR EMBL; AK166569; BAE38860.1; -; mRNA.
DR EMBL; BC076599; AAH76599.1; -; mRNA.
DR CCDS; CCDS20713.1; -.
DR RefSeq; NP_062617.2; NM_019643.3.
DR RefSeq; XP_006507137.1; XM_006507074.3.
DR RefSeq; XP_006507138.1; XM_006507075.2.
DR AlphaFoldDB; Q8C8M1; -.
DR BioGRID; 207890; 15.
DR ComplexPortal; CPX-3441; SIN3A histone deacetylase complex, ES cell-specific variant.
DR IntAct; Q8C8M1; 15.
DR STRING; 10090.ENSMUSP00000107188; -.
DR iPTMnet; Q8C8M1; -.
DR PhosphoSitePlus; Q8C8M1; -.
DR EPD; Q8C8M1; -.
DR MaxQB; Q8C8M1; -.
DR PaxDb; Q8C8M1; -.
DR PeptideAtlas; Q8C8M1; -.
DR PRIDE; Q8C8M1; -.
DR ProteomicsDB; 257224; -.
DR Antibodypedia; 24652; 48 antibodies from 12 providers.
DR Ensembl; ENSMUST00000054080; ENSMUSP00000050952; ENSMUSG00000039985.
DR Ensembl; ENSMUST00000081956; ENSMUSP00000107188; ENSMUSG00000039985.
DR Ensembl; ENSMUST00000111562; ENSMUSP00000107187; ENSMUSG00000039985.
DR Ensembl; ENSMUST00000204435; ENSMUSP00000145487; ENSMUSG00000039985.
DR GeneID; 56306; -.
DR KEGG; mmu:56306; -.
DR UCSC; uc009etq.1; mouse.
DR CTD; 58516; -.
DR MGI; MGI:1929091; Sinhcaf.
DR VEuPathDB; HostDB:ENSMUSG00000039985; -.
DR eggNOG; ENOG502QSAG; Eukaryota.
DR GeneTree; ENSGT00390000006485; -.
DR HOGENOM; CLU_049492_0_0_1; -.
DR InParanoid; Q8C8M1; -.
DR OMA; TPTCEEN; -.
DR OrthoDB; 1330424at2759; -.
DR PhylomeDB; Q8C8M1; -.
DR TreeFam; TF323911; -.
DR BioGRID-ORCS; 56306; 9 hits in 67 CRISPR screens.
DR ChiTaRS; Sinhcaf; mouse.
DR PRO; PR:Q8C8M1; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q8C8M1; protein.
DR Bgee; ENSMUSG00000039985; Expressed in undifferentiated genital tubercle and 65 other tissues.
DR ExpressionAtlas; Q8C8M1; baseline and differential.
DR Genevisible; Q8C8M1; MM.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0016580; C:Sin3 complex; ISS:UniProtKB.
DR GO; GO:0045596; P:negative regulation of cell differentiation; IMP:UniProtKB.
DR GO; GO:0030336; P:negative regulation of cell migration; ISS:UniProtKB.
DR GO; GO:1902455; P:negative regulation of stem cell population maintenance; IC:ComplexPortal.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IC:ComplexPortal.
DR GO; GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IC:ComplexPortal.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:UniProtKB.
DR GO; GO:1902459; P:positive regulation of stem cell population maintenance; IC:ComplexPortal.
DR InterPro; IPR026065; FAM60A.
DR PANTHER; PTHR13422; PTHR13422; 1.
DR Pfam; PF15396; FAM60A; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..221
FT /note="SIN3-HDAC complex-associated factor"
FT /id="PRO_0000187087"
FT REGION 110..153
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 201..221
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 122..153
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 29
FT /note="T -> Q (in Ref. 1; AAB09547)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 221 AA; 24829 MW; 6A7CD621D86B72C8 CRC64;
MFGFHKPKMY RSIEGCCICR AKSSSSRFTD SKRYEKDFQS CFGLHETRSG DICNACVLLV
KRWKKLPAGS KKNWNHVVDA RAGPSLKTTL KPKKVKTLSG NRMKSNQISK LQKEFKRHNS
DAHSTTSSAS PAQSPCYSNQ SDEGSDTEMA SSSNRTPVFS FLDLTYWKRQ KICCGIIYKG
RFGEVLIDTH LFKPCCSSKK AAAEKPEEQG PAPLPISTQE W
