ID   SHE2_YEAS1              Reviewed;         246 AA.
AC   B3LQW9;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=SWI5-dependent HO expression protein 2;
GN   Name=SHE2; ORFNames=SCRG_03894;
OS   Saccharomyces cerevisiae (strain RM11-1a) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=285006;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RM11-1a;
RG   The Broad Institute Genome Sequencing Platform;
RA   Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Cuomo C.,
RA   Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M.,
RA   Mauceli E.W., Brockman W., MacCallum I.A., Rounsley S., Young S.K.,
RA   LaButti K., Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R.,
RA   Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J.,
RA   O'Leary S., Kodira C.D., Zeng Q., Yandava C., Alvarado L., Pratt S.,
RA   Kruglyak L.;
RT   "Annotation of the Saccharomyces cerevisiae RM11-1a genome.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA-binding protein that binds specific mRNAs including the
CC       ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the
CC       mRNA localization machinery that restricts accumulation of certain
CC       proteins to the bud and in the daughter cell. Recruits the MYO4-SHE3
CC       complex to the ASH1 mRNA. Recruits also LOC1 and PUF6 to ASH1 mRNA,
CC       which are required for translational repression of this mRNA (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer and homotetramer. Interacts with LOC1, MYO4, PUF6,
CC       SHE3 and with RNA pol II subunits RPO21, SPT4 and SPT5. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P36068}. Nucleus
CC       {ECO:0000250|UniProtKB:P36068}. Note=Shuttles between the nucleus and
CC       cytoplasm and is exported in an mRNA-dependent manner. The presence in
CC       the nucleus is essential for PUF6 and LOC1 to bind the ASH1 mRNA.
CC       {ECO:0000250|UniProtKB:P36068}.
CC   -!- SIMILARITY: Belongs to the SHE2 family. {ECO:0000305}.
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DR   EMBL; CH408051; EDV12972.1; -; Genomic_DNA.
DR   AlphaFoldDB; B3LQW9; -.
DR   SMR; B3LQW9; -.
DR   EnsemblFungi; EDV12972; EDV12972; SCRG_03894.
DR   HOGENOM; CLU_1129832_0_0_1; -.
DR   Proteomes; UP000008335; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.200.20; -; 1.
DR   InterPro; IPR024261; RNA-bd_She2.
DR   InterPro; IPR036827; She2_dom_sf.
DR   Pfam; PF11435; She2p; 1.
DR   SUPFAM; SSF116942; SSF116942; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; mRNA transport; Nucleus; RNA-binding; Transport.
FT   CHAIN           1..246
FT                   /note="SWI5-dependent HO expression protein 2"
FT                   /id="PRO_0000408921"
FT   MOTIF           15..23
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   246 AA;  28251 MW;  5514B374655EFDBB CRC64;
     MSKDKDIKVT PGTCELVEQI LALLSRYLSS YIHVLNKFIS HLRRVATLRF ERTTLIKFVK
     KLRFYNDCVL SYNASEFINE GKNELDPEAD SFDKVILPIA SMFVKCVETF DLLNYYLTQS
     LQKEILSKTL NEDLTLTAES ILAIDDTYNH FVKFSQWMIE SLRIGSNLLD LEVVQFAIKC
     ADEDGTNIGE TDNIFLQEIL PVNSEEEFQT LSAAWHSILD GKLSALDEEF DVVATKWHDK
     FGKLKN