ID   SHE2_YEAS6              Reviewed;         246 AA.
AC   B5VM30;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=SWI5-dependent HO expression protein 2;
GN   Name=SHE2; ORFNames=AWRI1631_110940;
OS   Saccharomyces cerevisiae (strain AWRI1631) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=545124;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AWRI1631;
RX   PubMed=18778279; DOI=10.1111/j.1567-1364.2008.00434.x;
RA   Borneman A.R., Forgan A.H., Pretorius I.S., Chambers P.J.;
RT   "Comparative genome analysis of a Saccharomyces cerevisiae wine strain.";
RL   FEMS Yeast Res. 8:1185-1195(2008).
CC   -!- FUNCTION: RNA-binding protein that binds specific mRNAs including the
CC       ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the
CC       mRNA localization machinery that restricts accumulation of certain
CC       proteins to the bud and in the daughter cell. Recruits the MYO4-SHE3
CC       complex to the ASH1 mRNA. Recruits also LOC1 and PUF6 to ASH1 mRNA,
CC       which are required for translational repression of this mRNA (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer and homotetramer. Interacts with LOC1, MYO4, PUF6,
CC       SHE3 and with RNA pol II subunits RPO21, SPT4 and SPT5. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P36068}. Nucleus
CC       {ECO:0000250|UniProtKB:P36068}. Note=Shuttles between the nucleus and
CC       cytoplasm and is exported in an mRNA-dependent manner. The presence in
CC       the nucleus is essential for PUF6 and LOC1 to bind the ASH1 mRNA.
CC       {ECO:0000250|UniProtKB:P36068}.
CC   -!- SIMILARITY: Belongs to the SHE2 family. {ECO:0000305}.
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DR   EMBL; ABSV01001452; EDZ71015.1; -; Genomic_DNA.
DR   AlphaFoldDB; B5VM30; -.
DR   SMR; B5VM30; -.
DR   Proteomes; UP000008988; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.200.20; -; 1.
DR   InterPro; IPR024261; RNA-bd_She2.
DR   InterPro; IPR036827; She2_dom_sf.
DR   Pfam; PF11435; She2p; 1.
DR   SUPFAM; SSF116942; SSF116942; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; mRNA transport; Nucleus; RNA-binding; Transport.
FT   CHAIN           1..246
FT                   /note="SWI5-dependent HO expression protein 2"
FT                   /id="PRO_0000408923"
FT   MOTIF           15..23
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   246 AA;  28251 MW;  5514B374655EFDBB CRC64;
     MSKDKDIKVT PGTCELVEQI LALLSRYLSS YIHVLNKFIS HLRRVATLRF ERTTLIKFVK
     KLRFYNDCVL SYNASEFINE GKNELDPEAD SFDKVILPIA SMFVKCVETF DLLNYYLTQS
     LQKEILSKTL NEDLTLTAES ILAIDDTYNH FVKFSQWMIE SLRIGSNLLD LEVVQFAIKC
     ADEDGTNIGE TDNIFLQEIL PVNSEEEFQT LSAAWHSILD GKLSALDEEF DVVATKWHDK
     FGKLKN