ID   SHE2_YEAS7              Reviewed;         246 AA.
AC   A6ZZJ0;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   25-MAY-2022, entry version 44.
DE   RecName: Full=SWI5-dependent HO expression protein 2;
GN   Name=SHE2; ORFNames=SCY_3250;
OS   Saccharomyces cerevisiae (strain YJM789) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=307796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YJM789;
RX   PubMed=17652520; DOI=10.1073/pnas.0701291104;
RA   Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z.,
RA   Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X.,
RA   Jia P., Luedi P., Oefner P.J., David L., Dietrich F.S., Li Y., Davis R.W.,
RA   Steinmetz L.M.;
RT   "Genome sequencing and comparative analysis of Saccharomyces cerevisiae
RT   strain YJM789.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007).
CC   -!- FUNCTION: RNA-binding protein that binds specific mRNAs including the
CC       ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the
CC       mRNA localization machinery that restricts accumulation of certain
CC       proteins to the bud and in the daughter cell. Recruits the MYO4-SHE3
CC       complex to the ASH1 mRNA. Recruits also LOC1 and PUF6 to ASH1 mRNA,
CC       which are required for translational repression of this mRNA (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer and homotetramer. Interacts with LOC1, MYO4, PUF6,
CC       SHE3 and with RNA pol II subunits RPO21, SPT4 and SPT5. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P36068}. Nucleus
CC       {ECO:0000250|UniProtKB:P36068}. Note=Shuttles between the nucleus and
CC       cytoplasm and is exported in an mRNA-dependent manner. The presence in
CC       the nucleus is essential for PUF6 and LOC1 to bind the ASH1 mRNA.
CC       {ECO:0000250|UniProtKB:P36068}.
CC   -!- SIMILARITY: Belongs to the SHE2 family. {ECO:0000305}.
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DR   EMBL; AAFW02000151; EDN60038.1; -; Genomic_DNA.
DR   AlphaFoldDB; A6ZZJ0; -.
DR   SMR; A6ZZJ0; -.
DR   EnsemblFungi; EDN60038; EDN60038; SCY_3250.
DR   HOGENOM; CLU_1129832_0_0_1; -.
DR   Proteomes; UP000007060; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.200.20; -; 1.
DR   InterPro; IPR024261; RNA-bd_She2.
DR   InterPro; IPR036827; She2_dom_sf.
DR   Pfam; PF11435; She2p; 1.
DR   SUPFAM; SSF116942; SSF116942; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; mRNA transport; Nucleus; RNA-binding; Transport.
FT   CHAIN           1..246
FT                   /note="SWI5-dependent HO expression protein 2"
FT                   /id="PRO_0000408924"
FT   MOTIF           15..23
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   246 AA;  28265 MW;  A19DD6E23C3FDC03 CRC64;
     MSKDKDIKVT PGTCELIEQI LALLSRYLSS YIHVLNKFIS HLRRVATLRF ERTTLIKFVK
     KLRFYNDCVL SYNASEFINE GKNELDPEAD SFDKVILPIA SMFVKCVETF DLLNYYLTQS
     LQKEILSKTL NEDLTLTAES ILAIDDTYNH FVKFSQWMIE SLRIGSNLLD LEVVQFAIKC
     ADEDGTNIGE TDNIFLQEIL PVNSEEEFQT LSAAWHSILD GKLSALDEEF DVVATKWHDK
     FGKLKN