ID   BGLS_TRIRP              Reviewed;         493 AA.
AC   P26204;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Non-cyanogenic beta-glucosidase;
DE            EC=3.2.1.21;
DE   Flags: Precursor;
OS   Trifolium repens (Creeping white clover).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Trifolium.
OX   NCBI_TaxID=3899;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 135-157.
RC   STRAIN=S100 (EG); TISSUE=Leaf;
RX   PubMed=1907511; DOI=10.1007/bf00039495;
RA   Oxtoby E., Dunn M.A., Pancoro A., Hughes M.A.;
RT   "Nucleotide and derived amino acid sequence of the cyanogenic beta-
RT   glucosidase (linamarase) from white clover (Trifolium repens L.).";
RL   Plant Mol. Biol. 17:209-219(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal, non-reducing beta-D-glucosyl residues
CC         with release of beta-D-glucose.; EC=3.2.1.21;
CC   -!- TISSUE SPECIFICITY: Leaves.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family. {ECO:0000305}.
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DR   EMBL; X56734; CAA40058.1; -; mRNA.
DR   PIR; S16581; GLJY31.
DR   AlphaFoldDB; P26204; -.
DR   SMR; P26204; -.
DR   CAZy; GH1; Glycoside Hydrolase Family 1.
DR   GO; GO:0008422; F:beta-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102483; F:scopolin beta-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR001360; Glyco_hydro_1.
DR   InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR10353; PTHR10353; 1.
DR   Pfam; PF00232; Glyco_hydro_1; 1.
DR   PRINTS; PR00131; GLHYDRLASE1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00572; GLYCOSYL_HYDROL_F1_1; 1.
DR   PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glycoprotein; Glycosidase; Hydrolase; Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..493
FT                   /note="Non-cyanogenic beta-glucosidase"
FT                   /id="PRO_0000011764"
FT   ACT_SITE        204
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        422
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10055"
FT   BINDING         54
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         158
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         203
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         346
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         471
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         478..479
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        34
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        335
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        371
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        412
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   493 AA;  55960 MW;  B6B3BAA5BBFFCB8F CRC64;
     MDFIVAIFAL FVISSFTITS TNAVEASTLL DIGNLSRSSF PRGFIFGAGS SAYQFEGAVN
     EGGRGPSIWD TFTHKYPEKI RDGSNADITV DQYHRYKEDV GIMKDQNMDS YRFSISWPRI
     LPKGKLSGGI NHEGIKYYNN LINELLANGI QPFVTLFHWD LPQVLEDEYG GFLNSGVIND
     FRDYTDLCFK EFGDRVRYWS TLNEPWVFSN SGYALGTNAP GRCSASNVAK PGDSGTGPYI
     VTHNQILAHA EAVHVYKTKY QAYQKGKIGI TLVSNWLMPL DDNSIPDIKA AERSLDFQFG
     LFMEQLTTGD YSKSMRRIVK NRLPKFSKFE SSLVNGSFDF IGINYYSSSY ISNAPSHGNA
     KPSYSTNPMT NISFEKHGIP LGPRAASIWI YVYPYMFIQE DFEIFCYILK INITILQFSI
     TENGMNEFND ATLPVEEALL NTYRIDYYYR HLYYIRSAIR AGSNVKGFYA WSFLDCNEWF
     AGFTVRFGLN FVD