SHEP_DROME
ID SHEP_DROME Reviewed; 590 AA.
AC Q8MSV2; E1JID7; M9ND50; M9PEP6; Q7KU60; Q86BS4; Q8SWY6; Q95S20; Q95S52;
AC Q9VRK3; Q9VRK5;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 2.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Protein alan shepard;
GN Name=shep {ECO:0000312|FlyBase:FBgn0052423}; ORFNames=CG32423;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|EMBL:AAF50788.5}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAF50788.5}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAM49933.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A; B AND E), AND
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 398-590 (ISOFORMS A/E/F/I).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM49933.1};
RC TISSUE=Embryo {ECO:0000269|PubMed:12537569},
RC Head {ECO:0000269|PubMed:12537569}, and
RC Ovary {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP IDENTIFICATION, AND FUNCTION.
RX PubMed=16594976; DOI=10.1111/j.1601-183x.2005.00154.x;
RA Armstrong J.D., Texada M.J., Munjaal R., Baker D.A., Beckingham K.M.;
RT "Gravitaxis in Drosophila melanogaster: a forward genetic screen.";
RL Genes Brain Behav. 5:222-239(2006).
RN [5] {ECO:0000305}
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-125 AND TYR-142, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
RN [6] {ECO:0000305}
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-5, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC TISSUE=Embryo {ECO:0000269|PubMed:18327897};
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Has a role in the perception of gravity.
CC {ECO:0000269|PubMed:16594976}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=6;
CC Name=F;
CC IsoId=Q8MSV2-5; Sequence=Displayed;
CC Name=A {ECO:0000269|PubMed:10731132};
CC IsoId=Q8MSV2-1; Sequence=VSP_054214;
CC Name=B {ECO:0000269|PubMed:10731132}; Synonyms=D
CC {ECO:0000269|PubMed:10731132};
CC IsoId=Q8MSV2-2; Sequence=VSP_054213, VSP_054214, VSP_054215,
CC VSP_054216, VSP_054219;
CC Name=E {ECO:0000269|PubMed:10731132}; Synonyms=G;
CC IsoId=Q8MSV2-4; Sequence=VSP_054212, VSP_054216;
CC Name=I;
CC IsoId=Q8MSV2-6; Sequence=VSP_054212, VSP_054217;
CC Name=H;
CC IsoId=Q8MSV2-7; Sequence=VSP_054212, VSP_054217, VSP_054218;
CC -!- MISCELLANEOUS: Named after Alan Bartlett Shepard, Jr. who was the
CC second person and the first American in space and the fifth person to
CC walk on the moon. {ECO:0000269|PubMed:16594976}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL28502.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAL28543.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE014296; AAF50788.5; -; Genomic_DNA.
DR EMBL; AE014296; AAF50790.2; -; Genomic_DNA.
DR EMBL; AE014296; AAF50792.4; -; Genomic_DNA.
DR EMBL; AE014296; AAN12240.1; -; Genomic_DNA.
DR EMBL; AE014296; ACZ94622.1; -; Genomic_DNA.
DR EMBL; AE014296; AFH04307.1; -; Genomic_DNA.
DR EMBL; AE014296; AGB94133.1; -; Genomic_DNA.
DR EMBL; AE014296; AGB94134.1; -; Genomic_DNA.
DR EMBL; AY060954; AAL28502.1; ALT_INIT; mRNA.
DR EMBL; AY060995; AAL28543.1; ALT_FRAME; mRNA.
DR EMBL; AY094948; AAM11301.1; -; mRNA.
DR EMBL; AY118564; AAM49933.1; -; mRNA.
DR RefSeq; NP_001163350.1; NM_001169879.2. [Q8MSV2-4]
DR RefSeq; NP_001246636.1; NM_001259707.1. [Q8MSV2-5]
DR RefSeq; NP_001261438.1; NM_001274509.1. [Q8MSV2-4]
DR RefSeq; NP_001261439.1; NM_001274510.1. [Q8MSV2-7]
DR RefSeq; NP_729054.3; NM_168111.4. [Q8MSV2-1]
DR RefSeq; NP_729055.1; NM_168112.4. [Q8MSV2-2]
DR RefSeq; NP_729056.1; NM_168113.3. [Q8MSV2-2]
DR RefSeq; NP_729057.3; NM_168114.5. [Q8MSV2-6]
DR AlphaFoldDB; Q8MSV2; -.
DR SMR; Q8MSV2; -.
DR BioGRID; 64073; 17.
DR IntAct; Q8MSV2; 30.
DR STRING; 7227.FBpp0301014; -.
DR iPTMnet; Q8MSV2; -.
DR PaxDb; Q8MSV2; -.
DR PRIDE; Q8MSV2; -.
DR ABCD; Q8MSV2; 5 sequenced antibodies.
DR DNASU; 38605; -.
DR EnsemblMetazoa; FBtr0077172; FBpp0076875; FBgn0052423. [Q8MSV2-1]
DR EnsemblMetazoa; FBtr0077173; FBpp0076876; FBgn0052423. [Q8MSV2-2]
DR EnsemblMetazoa; FBtr0077174; FBpp0076877; FBgn0052423. [Q8MSV2-2]
DR EnsemblMetazoa; FBtr0301599; FBpp0290814; FBgn0052423. [Q8MSV2-4]
DR EnsemblMetazoa; FBtr0308859; FBpp0301014; FBgn0052423. [Q8MSV2-5]
DR EnsemblMetazoa; FBtr0333287; FBpp0305482; FBgn0052423. [Q8MSV2-4]
DR EnsemblMetazoa; FBtr0333288; FBpp0305483; FBgn0052423. [Q8MSV2-7]
DR EnsemblMetazoa; FBtr0333289; FBpp0305484; FBgn0052423. [Q8MSV2-6]
DR GeneID; 38605; -.
DR KEGG; dme:Dmel_CG32423; -.
DR UCSC; CG32423-RA; d. melanogaster. [Q8MSV2-5]
DR UCSC; CG32423-RB; d. melanogaster.
DR UCSC; CG32423-RC; d. melanogaster.
DR CTD; 38605; -.
DR FlyBase; FBgn0052423; shep.
DR VEuPathDB; VectorBase:FBgn0052423; -.
DR eggNOG; KOG4733; Eukaryota.
DR GeneTree; ENSGT00940000173832; -.
DR InParanoid; Q8MSV2; -.
DR OMA; HTMPLGD; -.
DR PhylomeDB; Q8MSV2; -.
DR BioGRID-ORCS; 38605; 1 hit in 3 CRISPR screens.
DR GenomeRNAi; 38605; -.
DR PRO; PR:Q8MSV2; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0052423; Expressed in brain and 59 other tissues.
DR Genevisible; Q8MSV2; DM.
DR GO; GO:0005829; C:cytosol; HDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; ISS:FlyBase.
DR GO; GO:0008143; F:poly(A) binding; IBA:GO_Central.
DR GO; GO:0008266; F:poly(U) RNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0008344; P:adult locomotory behavior; IMP:FlyBase.
DR GO; GO:0008050; P:female courtship behavior; IMP:FlyBase.
DR GO; GO:0042332; P:gravitaxis; IMP:FlyBase.
DR GO; GO:0007552; P:metamorphosis; IMP:FlyBase.
DR GO; GO:0016322; P:neuron remodeling; IMP:FlyBase.
DR GO; GO:0034976; P:response to endoplasmic reticulum stress; IMP:FlyBase.
DR GO; GO:0009629; P:response to gravity; IMP:UniProtKB.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR002343; Hud_Sxl_RNA.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 2.
DR PRINTS; PR00961; HUDSXLRNA.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome; Repeat;
KW RNA-binding.
FT CHAIN 1..590
FT /note="Protein alan shepard"
FT /id="PRO_0000379499"
FT DOMAIN 243..316
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 322..401
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..66
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 189..237
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 563..590
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 36..66
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 563..579
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 5
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 125
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT MOD_RES 142
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT VAR_SEQ 1..222
FT /note="Missing (in isoform E, isoform H and isoform I)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_054212"
FT VAR_SEQ 1..95
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:10731132,
FT ECO:0000303|PubMed:12537569"
FT /id="VSP_054213"
FT VAR_SEQ 169..180
FT /note="Missing (in isoform A and isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_054214"
FT VAR_SEQ 284..285
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_054215"
FT VAR_SEQ 315
FT /note="K -> KVGIWVLHRPAI (in isoform B and isoform E)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_054216"
FT VAR_SEQ 316..317
FT /note="QQ -> VGIWVLHRPAI (in isoform I and isoform H)"
FT /evidence="ECO:0000305"
FT /id="VSP_054217"
FT VAR_SEQ 483..488
FT /note="Missing (in isoform H)"
FT /evidence="ECO:0000305"
FT /id="VSP_054218"
FT VAR_SEQ 543
FT /note="S -> SNFSPSLQ (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_054219"
SQ SEQUENCE 590 AA; 62175 MW; 7700D1E378A5255A CRC64;
MHPRYSPAPP PQQQQQMGGP PHQQQGGGGG GGVSMRGPSN AQQLPPQIPR SQNYSNGSSS
SAAAAPLTSR SAFPGAPLTA SAVALKGALP QRPPAMTSPA AAAAGAALAA GAPYRGAASW
TPQGYAPAAA AAAAAVAQQA AYRYTAPLPQ PAYAAYTPHT ATTPATTTVS FLSQPVDYYW
YGQRVPTAAS PSNTNSSSSS NTGSQSGTLS TSLSNTTNTN TNMGPNGTVQ NQNQQGGEQL
SKTNLYIRGL QQGTTDKDLV NMCAQYGTII STKAILDKTT NKCKGYGFVD FEQPAFAECA
VKGLQGKGVQ AQMAKQQEQD PTNLYIANLP PHFKETDLEA MLSKYGQVVS TRILRDQQMN
SKGVGFARME SREKCEQIIQ MFNGNTIPGA KDPLLVKFAD GGPKKKNLFK TPDPNARAWR
DVSAEGIPVA YDPTMQQNGV SVNVGTPIGV PYSRFSAPQV GGYPVAGSQW IPGYMMTQVD
DQTSYSPQYM QMAAAPPLGV TSYKPEAVNQ VQPRGISMMV SGDTGVPYGT MMPQLATLQI
GNSYISPTYP YYAPPPTIIP TMPMTDSEQA STAASPDEAY TQYPHQAAPK
