SHF_HUMAN
ID SHF_HUMAN Reviewed; 423 AA.
AC Q7M4L6; Q96IE8;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=SH2 domain-containing adapter protein F;
GN Name=SHF {ECO:0000312|EMBL:AAH07586.1};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1] {ECO:0000305, ECO:0000312|PIR:JC7552}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH PDGFA,
RP TISSUE SPECIFICITY, AND PHOSPHORYLATION.
RC TISSUE=Skeletal muscle {ECO:0000269|PubMed:11095946};
RX PubMed=11095946; DOI=10.1006/bbrc.2000.3847;
RA Lindholm C.K., Frantz J.D., Shoelson S.E., Welsh M.;
RT "Shf, a Shb-like adapter protein, is involved in PDGF-alpha-receptor
RT regulation of apoptosis.";
RL Biochem. Biophys. Res. Commun. 278:537-543(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16572171; DOI=10.1038/nature04601;
RA Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J.,
RA Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E.,
RA Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B.,
RA Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R.,
RA O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B.,
RA Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S.,
RA Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.;
RT "Analysis of the DNA sequence and duplication history of human chromosome
RT 15.";
RL Nature 440:671-675(2006).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAH07586.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 144-423 (ISOFORM 2).
RC TISSUE=Neuroblastoma {ECO:0000312|EMBL:AAH07586.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Adapter protein which may play a role in the regulation of
CC apoptosis in response to PDGF. {ECO:0000269|PubMed:11095946}.
CC -!- SUBUNIT: Interacts with phosphorylated 'Tyr-720' of PDGFRA via its SH2
CC domain. {ECO:0000269|PubMed:11095946}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1 {ECO:0000269|PubMed:11095946};
CC IsoId=Q7M4L6-1; Sequence=Displayed;
CC Name=2 {ECO:0000269|PubMed:15489334};
CC IsoId=Q7M4L6-2; Sequence=VSP_052699;
CC -!- TISSUE SPECIFICITY: Expressed in skeletal muscle, brain, liver,
CC prostate, testis, ovary, small intestine and colon.
CC {ECO:0000269|PubMed:11095946}.
CC -!- PTM: May become phosphorylated upon binding to PDGFRA.
CC {ECO:0000269|PubMed:11095946}.
CC -!- MISCELLANEOUS: The sequence described in PubMed:11095946 is over-
CC extended by 57 aa at the N-terminus due to the presence of an
CC uncorrected 5' mismatch compared to the reference genome sequence.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC051619; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC007586; AAH07586.1; -; mRNA.
DR CCDS; CCDS10120.2; -. [Q7M4L6-1]
DR PIR; JC7552; JC7552.
DR AlphaFoldDB; Q7M4L6; -.
DR SMR; Q7M4L6; -.
DR BioGRID; 124730; 3.
DR IntAct; Q7M4L6; 38.
DR STRING; 9606.ENSP00000290894; -.
DR iPTMnet; Q7M4L6; -.
DR PhosphoSitePlus; Q7M4L6; -.
DR BioMuta; SHF; -.
DR DMDM; 308153498; -.
DR EPD; Q7M4L6; -.
DR MassIVE; Q7M4L6; -.
DR PaxDb; Q7M4L6; -.
DR PeptideAtlas; Q7M4L6; -.
DR PRIDE; Q7M4L6; -.
DR ProteomicsDB; 68869; -. [Q7M4L6-1]
DR ProteomicsDB; 68870; -. [Q7M4L6-2]
DR Antibodypedia; 42470; 110 antibodies from 19 providers.
DR Ensembl; ENST00000290894.12; ENSP00000290894.8; ENSG00000138606.20. [Q7M4L6-1]
DR UCSC; uc001zuy.3; human. [Q7M4L6-1]
DR GeneCards; SHF; -.
DR HGNC; HGNC:25116; SHF.
DR HPA; ENSG00000138606; Tissue enhanced (brain).
DR neXtProt; NX_Q7M4L6; -.
DR OpenTargets; ENSG00000138606; -.
DR VEuPathDB; HostDB:ENSG00000138606; -.
DR eggNOG; ENOG502QTRD; Eukaryota.
DR GeneTree; ENSGT00940000159452; -.
DR InParanoid; Q7M4L6; -.
DR PhylomeDB; Q7M4L6; -.
DR TreeFam; TF325799; -.
DR PathwayCommons; Q7M4L6; -.
DR SignaLink; Q7M4L6; -.
DR ChiTaRS; SHF; human.
DR Pharos; Q7M4L6; Tbio.
DR PRO; PR:Q7M4L6; -.
DR Proteomes; UP000005640; Chromosome 15.
DR RNAct; Q7M4L6; protein.
DR Bgee; ENSG00000138606; Expressed in cerebellar hemisphere and 112 other tissues.
DR ExpressionAtlas; Q7M4L6; baseline and differential.
DR Genevisible; Q7M4L6; HS.
DR GO; GO:0001784; F:phosphotyrosine residue binding; IBA:GO_Central.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR CDD; cd10392; SH2_SHF; 1.
DR Gene3D; 3.30.505.10; -; 1.
DR InterPro; IPR000980; SH2.
DR InterPro; IPR036860; SH2_dom_sf.
DR InterPro; IPR035043; SHF.
DR InterPro; IPR035044; SHF_SH2.
DR PANTHER; PTHR15127:SF28; PTHR15127:SF28; 1.
DR Pfam; PF00017; SH2; 1.
DR PRINTS; PR00401; SH2DOMAIN.
DR SMART; SM00252; SH2; 1.
DR SUPFAM; SSF55550; SSF55550; 1.
DR PROSITE; PS50001; SH2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Apoptosis; Phosphoprotein; Reference proteome;
KW SH2 domain.
FT CHAIN 1..423
FT /note="SH2 domain-containing adapter protein F"
FT /id="PRO_0000322558"
FT DOMAIN 323..418
FT /note="SH2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00191"
FT REGION 1..87
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 110..208
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 225..312
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 35..79
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 185..208
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 201
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q8CG80"
FT VAR_SEQ 218..264
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_052699"
FT CONFLICT 177
FT /note="A -> P (in Ref. 3; AAH07586)"
FT /evidence="ECO:0000305"
FT CONFLICT 377..378
FT /note="EH -> NN (in Ref. 1; no nucleotide entry)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 423 AA; 46768 MW; B63C06255825CC6C CRC64;
MQQEGGPVRS APCRTGTLEG SRQGSPGHRK RASPKGSLSS AQPHSWMLTP SPLNSHCAHR
EPISSSPQPV ANGPKQKKKS NWRSTTRLRI IRLRDRLEPR PLAILEDYAD PFDVQETGEG
SAGASGAPEK VPENDGYMEP YEAQKMMAEI RGSKETATQP LPLYDTPYEP EEDGATAEGE
GAPWPRESRL PEDDERPPEE YDQPWEWKKE RISKAFAVDI KVIKDLPWPP PVGQLDSSPS
LPDGDRDISG PASPLPEPSL EDSSAQFEGP EKSCLSPGRE EKGRLPPRLS AGNPKSAKPL
SMEPSSPLGE WTDPALPLEN QVWYHGAISR TDAENLLRLC KEASYLVRNS ETSKNDFSLS
LKSSQGFMHM KLSRTKEHKY VLGQNSPPFS SVPEIVHHYA SRKLPIKGAE HMSLLYPVAI
RTL
