SHF_MOUSE
ID SHF_MOUSE Reviewed; 238 AA.
AC Q8CG80; A2AQ90; A2AQ91;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=SH2 domain-containing adapter protein F;
GN Name=Shf {ECO:0000312|MGI:MGI:3613669};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2] {ECO:0000312|EMBL:AAH42839.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N {ECO:0000312|EMBL:AAH42839.1};
RC TISSUE=Mammary tumor {ECO:0000312|EMBL:AAH42839.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-64, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=18034455; DOI=10.1021/pr0701254;
RA Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.;
RT "Large-scale identification and evolution indexing of tyrosine
RT phosphorylation sites from murine brain.";
RL J. Proteome Res. 7:311-318(2008).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Adapter protein which may play a role in the regulation of
CC apoptosis in response to PDGF. {ECO:0000250|UniProtKB:Q7M4L6}.
CC -!- SUBUNIT: Interacts with phosphorylated 'Tyr-720' of PDGFRA via its SH2
CC domain. {ECO:0000250|UniProtKB:Q7M4L6}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=Additional isoforms seem to exist. {ECO:0000305};
CC Name=1 {ECO:0000269|PubMed:15489334};
CC IsoId=Q8CG80-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CG80-2; Sequence=VSP_052700;
CC -!- PTM: May become phosphorylated upon binding to PDGFRA.
CC {ECO:0000250|UniProtKB:Q7M4L6}.
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DR EMBL; AL844566; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC042839; AAH42839.1; -; mRNA.
DR CCDS; CCDS16662.1; -. [Q8CG80-1]
DR RefSeq; NP_001013851.2; NM_001013829.2. [Q8CG80-1]
DR AlphaFoldDB; Q8CG80; -.
DR SMR; Q8CG80; -.
DR STRING; 10090.ENSMUSP00000045135; -.
DR iPTMnet; Q8CG80; -.
DR PhosphoSitePlus; Q8CG80; -.
DR MaxQB; Q8CG80; -.
DR PaxDb; Q8CG80; -.
DR PRIDE; Q8CG80; -.
DR ProteomicsDB; 257001; -. [Q8CG80-1]
DR ProteomicsDB; 257002; -. [Q8CG80-2]
DR Antibodypedia; 42470; 110 antibodies from 19 providers.
DR Ensembl; ENSMUST00000048635; ENSMUSP00000045135; ENSMUSG00000033256. [Q8CG80-1]
DR Ensembl; ENSMUST00000110530; ENSMUSP00000106159; ENSMUSG00000033256. [Q8CG80-2]
DR Ensembl; ENSMUST00000110531; ENSMUSP00000106160; ENSMUSG00000033256. [Q8CG80-1]
DR GeneID; 435684; -.
DR KEGG; mmu:435684; -.
DR UCSC; uc008mar.1; mouse. [Q8CG80-1]
DR CTD; 90525; -.
DR MGI; MGI:3613669; Shf.
DR VEuPathDB; HostDB:ENSMUSG00000033256; -.
DR eggNOG; ENOG502QTRD; Eukaryota.
DR GeneTree; ENSGT00940000159452; -.
DR HOGENOM; CLU_029444_2_0_1; -.
DR InParanoid; Q8CG80; -.
DR OrthoDB; 1120795at2759; -.
DR PhylomeDB; Q8CG80; -.
DR TreeFam; TF325799; -.
DR BioGRID-ORCS; 435684; 2 hits in 72 CRISPR screens.
DR PRO; PR:Q8CG80; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8CG80; protein.
DR Bgee; ENSMUSG00000033256; Expressed in cerebellar cortex and 175 other tissues.
DR ExpressionAtlas; Q8CG80; baseline and differential.
DR Genevisible; Q8CG80; MM.
DR GO; GO:0001784; F:phosphotyrosine residue binding; IBA:GO_Central.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR CDD; cd10392; SH2_SHF; 1.
DR Gene3D; 3.30.505.10; -; 1.
DR InterPro; IPR000980; SH2.
DR InterPro; IPR036860; SH2_dom_sf.
DR InterPro; IPR035043; SHF.
DR InterPro; IPR035044; SHF_SH2.
DR PANTHER; PTHR15127:SF28; PTHR15127:SF28; 2.
DR Pfam; PF00017; SH2; 1.
DR PRINTS; PR00401; SH2DOMAIN.
DR SMART; SM00252; SH2; 1.
DR SUPFAM; SSF55550; SSF55550; 1.
DR PROSITE; PS50001; SH2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Apoptosis; Phosphoprotein; Reference proteome;
KW SH2 domain.
FT CHAIN 1..238
FT /note="SH2 domain-containing adapter protein F"
FT /id="PRO_0000322559"
FT DOMAIN 138..233
FT /note="SH2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00191"
FT REGION 1..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 85..121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 48..70
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 39
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 64
FT /note="Phosphotyrosine"
FT /evidence="ECO:0007744|PubMed:18034455"
FT VAR_SEQ 1..11
FT /note="MEPYEAQKMMA -> MQKHR (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_052700"
FT CONFLICT 87
FT /note="E -> EK (in Ref. 2; AAH42839)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 238 AA; 26893 MW; 9484D44D689C9ECB CRC64;
MEPYEAQKMM AEIRGSKETA AQPLPLYDTP YEPEDEGASP EGEGTPWPRE SRLPEDDERP
PEEYDQPWEW KKERISKAFA AQFEGSENCL SPGREEKGRL PPRLSAGNPK SAKPLGMEPS
SPLGEWTDPA LPLENQVWYH GAISRTDAEN LLRLCKEASY LVRNSETSKN DFSLSLKSSQ
GFMHMKLSRT KEHKYVLGQN SPPFSSVPEI VHHYASRKLP IKGAEHMSLL YPVAIRTL
