SHIA_ECOLI
ID SHIA_ECOLI Reviewed; 438 AA.
AC P76350;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Shikimate transporter;
GN Name=shiA; Synonyms=yeeM; OrderedLocusNames=b1981, JW1962;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=9524262; DOI=10.1016/s0378-1119(98)00043-2;
RA Whipp M.J., Camakaris H., Pittard A.J.;
RT "Cloning and analysis of the shiA gene, which encodes the shikimate
RT transport system of Escherichia coli K-12.";
RL Gene 209:185-192(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15919996}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Metabolite:H+
CC Symporter (MHS) family (TC 2.A.1.6) family. {ECO:0000305}.
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DR EMBL; U88529; AAC46271.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75045.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15801.1; -; Genomic_DNA.
DR PIR; G64962; G64962.
DR RefSeq; NP_416488.1; NC_000913.3.
DR RefSeq; WP_000378575.1; NZ_LN832404.1.
DR AlphaFoldDB; P76350; -.
DR SMR; P76350; -.
DR BioGRID; 4260400; 9.
DR STRING; 511145.b1981; -.
DR TCDB; 2.A.1.6.6; the major facilitator superfamily (mfs).
DR PaxDb; P76350; -.
DR PRIDE; P76350; -.
DR EnsemblBacteria; AAC75045; AAC75045; b1981.
DR EnsemblBacteria; BAA15801; BAA15801; BAA15801.
DR GeneID; 946495; -.
DR KEGG; ecj:JW1962; -.
DR KEGG; eco:b1981; -.
DR PATRIC; fig|1411691.4.peg.270; -.
DR EchoBASE; EB4148; -.
DR eggNOG; COG0477; Bacteria.
DR HOGENOM; CLU_001265_39_5_6; -.
DR InParanoid; P76350; -.
DR OMA; LWIRNGM; -.
DR PhylomeDB; P76350; -.
DR BioCyc; EcoCyc:SHIA-MON; -.
DR BioCyc; MetaCyc:SHIA-MON; -.
DR PRO; PR:P76350; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015530; F:shikimate transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0015733; P:shikimate transmembrane transport; IMP:EcoCyc.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004736; MHS_symport.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF07690; MFS_1; 1.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00883; 2A0106; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..438
FT /note="Shikimate transporter"
FT /id="PRO_0000050336"
FT TOPO_DOM 1..27
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 28..48
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 49..63
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..84
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 85..108
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 130..132
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 154..167
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 189..192
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 214..254
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 276..286
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 287..307
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 308..317
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..337
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 338..340
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..363
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 364..386
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 408..410
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 432..438
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 438 AA; 47817 MW; B7FFBBD5AFDAE8CC CRC64;
MDSTLISTRP DEGTLSLSRA RRAALGSFAG AVVDWYDFLL YGITAALVFN REFFPQVSPA
MGTLAAFATF GVGFLFRPLG GVIFGHFGDR LGRKRMLMLT VWMMGIATAL IGILPSFSTI
GWWAPILLVT LRAIQGFAVG GEWGGAALLS VESAPKNKKA FYSSGVQVGY GVGLLLSTGL
VSLISMMTTD EQFLSWGWRI PFLFSIVLVL GALWVRNGME ESAEFEQQQH YQAAAKKRIP
VIEALLRHPG AFLKIIALRL CELLTMYIVT AFALNYSTQN MGLPRELFLN IGLLVGGLSC
LTIPCFAWLA DRFGRRRVYI TGTLIGTLSA FPFFMALEAQ SIFWIVFFSI MLANIAHDMV
VCVQQPMFTE MFGASYRYSG AGVGYQVASV VGGGFTPFIA AALITYFAGN WHSVAIYLLA
GCLISAMTAL LMKDSQRA
