ID   SHKL1_NEMVE             Reviewed;          79 AA.
AC   P0DQR6;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 1.
DT   03-AUG-2022, entry version 3.
DE   RecName: Full=Neurotoxin ShK-like1 {ECO:0000303|PubMed:33060291};
DE   Flags: Precursor;
OS   Nematostella vectensis (Starlet sea anemone).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Edwardsiidae; Nematostella.
OX   NCBI_TaxID=45351;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 39-50 AND 63-73, FUNCTION,
RP   BIOASSAY, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, DEVELOPMENTAL STAGE, AND RECOMBINANT EXPRESSION.
RX   PubMed=33060291; DOI=10.1073/pnas.2011120117;
RA   Sachkova M.Y., Landau M., Surm J.M., Macrander J., Singer S.A.,
RA   Reitzel A.M., Moran Y.;
RT   "Toxin-like neuropeptides in the sea anemone Nematostella unravel
RT   recruitment from the nervous system to venom.";
RL   Proc. Natl. Acad. Sci. U.S.A. 117:27481-27492(2020).
RN   [2]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=29739837; DOI=10.1242/dev.162867;
RA   Warner J.F., Guerlais V., Amiel A.R., Johnston H., Nedoncelle K.,
RA   Roettinger E.;
RT   "NvERTx: a gene expression database to compare embryogenesis and
RT   regeneration in the sea anemone Nematostella vectensis.";
RL   Development 145:0-0(2018).
CC   -!- FUNCTION: Neurotoxin (PubMed:33060291). In vivo, induces contraction
CC       paralysis followed by death (within 2 hours) on zebrafish larvae
CC       (PubMed:33060291). Also induces body contraction in Nematostella 11-dpf
CC       polyps (PubMed:33060291). {ECO:0000269|PubMed:33060291}.
CC   -!- SUBCELLULAR LOCATION: Nematocyst {ECO:0000269|PubMed:33060291}.
CC       Secreted {ECO:0000269|PubMed:33060291}.
CC   -!- TISSUE SPECIFICITY: Expressed in nematocytes (in planulae and primary
CC       polyps). Is localized predominantly in the body column nematocytes and
CC       not in the tentacles (in primary polyps).
CC       {ECO:0000269|PubMed:33060291}.
CC   -!- DEVELOPMENTAL STAGE: Is highly detected in planulae and primary polyps
CC       (9d), and more weakly in both adult females and males (at protein
CC       level) (PubMed:33060291). Transcripts are abundant in larvae and
CC       significantly decrease later in the life cycle (PubMed:29739837).
CC       {ECO:0000269|PubMed:29739837, ECO:0000269|PubMed:33060291}.
CC   -!- WEB RESOURCE: Name=National Center for Biotechnology Information
CC       (NCBI);
CC       URL="https://www.ncbi.nlm.nih.gov/nuccore/HADP01047794.1/";
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DR   SMR; P0DQR6; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Nematocyst; Neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   PROPEP          26..35
FT                   /evidence="ECO:0000305|PubMed:33060291"
FT                   /id="PRO_0000453910"
FT   CHAIN           36..79
FT                   /note="Neurotoxin ShK-like1"
FT                   /evidence="ECO:0000305|PubMed:33060291"
FT                   /id="PRO_0000453911"
FT   DOMAIN          39..78
FT                   /note="ShKT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        39..78
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        47..71
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        56..75
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
SQ   SEQUENCE   79 AA;  8961 MW;  96BEB69E3DFCE71F CRC64;
     MSRKLLAVLM VCTFFLIAAS MGTNALPFHE GIERRAAKCV DKMPFVCMRK DIPAICKNRN
     HRSYAFIMDV CRKTCGQCT