SHKL2_NEMVE
ID SHKL2_NEMVE Reviewed; 92 AA.
AC A7SRR3;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 29-SEP-2021, sequence version 2.
DT 23-FEB-2022, entry version 30.
DE RecName: Full=Neuropeptide ShK-like2 {ECO:0000303|PubMed:33060291};
DE AltName: Full=ShK-like2a {ECO:0000303|PubMed:33060291};
DE AltName: Full=ShK-like2b {ECO:0000303|PubMed:33060291};
DE Flags: Precursor;
OS Nematostella vectensis (Starlet sea anemone).
OC Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC Edwardsiidae; Nematostella.
OX NCBI_TaxID=45351;
RN [1] {ECO:0000312|EMBL:EDO33598.1, ECO:0000312|Proteomes:UP000001593}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 2).
RC STRAIN=CH2 X CH6 {ECO:0000312|Proteomes:UP000001593};
RX PubMed=17615350; DOI=10.1126/science.1139158;
RA Putnam N.H., Srivastava M., Hellsten U., Dirks B., Chapman J., Salamov A.,
RA Terry A., Shapiro H., Lindquist E., Kapitonov V.V., Jurka J.,
RA Genikhovich G., Grigoriev I.V., Lucas S.M., Steele R.E., Finnerty J.R.,
RA Technau U., Martindale M.Q., Rokhsar D.S.;
RT "Sea anemone genome reveals ancestral eumetazoan gene repertoire and
RT genomic organization.";
RL Science 317:86-94(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 77-86, FUNCTION,
RP IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, RECOMBINANT EXPRESSION, AND ALTERNATIVE PROMOTER USAGE (ISOFORMS 1
RP AND 2).
RX PubMed=33060291; DOI=10.1073/pnas.2011120117;
RA Sachkova M.Y., Landau M., Surm J.M., Macrander J., Singer S.A.,
RA Reitzel A.M., Moran Y.;
RT "Toxin-like neuropeptides in the sea anemone Nematostella unravel
RT recruitment from the nervous system to venom.";
RL Proc. Natl. Acad. Sci. U.S.A. 117:27481-27492(2020).
RN [3]
RP DEVELOPMENTAL STAGE.
RX PubMed=29739837; DOI=10.1242/dev.162867;
RA Warner J.F., Guerlais V., Amiel A.R., Johnston H., Nedoncelle K.,
RA Roettinger E.;
RT "NvERTx: a gene expression database to compare embryogenesis and
RT regeneration in the sea anemone Nematostella vectensis.";
RL Development 145:0-0(2018).
CC -!- FUNCTION: In vivo, this neuropeptide induces contraction paralysis
CC followed by death (within 2 hours) on 4 zebrafish larvae on the 15
CC tested (PubMed:33060291). Also induces body contraction in Nematostella
CC 11-dpf polyps (PubMed:33060291). {ECO:0000269|PubMed:33060291}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative promoter usage; Named isoforms=2;
CC Name=1; Synonyms=ShK-like2a;
CC IsoId=A7SRR3-1; Sequence=Displayed;
CC Name=2; Synonyms=ShK-like2b;
CC IsoId=A7SRR3-2; Sequence=VSP_061205;
CC -!- TISSUE SPECIFICITY: [Isoform 1]: Transcripts are first expressed mostly
CC in the endoderm (with rare ectodermal cells) in the late planulae. They
CC are mostly expressed in endodermal ganglion cells in the body column
CC and tentacles in primary polyps, as well as in a small number of
CC ectodermal sensory neurons in tentacles and body wall. They are not
CC expressed in nematocytes. {ECO:0000269|PubMed:33060291}.
CC -!- TISSUE SPECIFICITY: [Isoform 2]: Transcripts are predominantly
CC expressed in ectodermal sensory neurons in early and late planulae.
CC They are expressed in endodermal ganglion cells in the body column and
CC tentacles in primary polyps, as well as in a small number of ectodermal
CC neurons in pharynx. They are not expressed in nematocytes.
CC {ECO:0000269|PubMed:33060291}.
CC -!- DEVELOPMENTAL STAGE: [Isoform 1]: Is moderately detected in planulae
CC and a little more detected in primary polyps (9d), as well as in both
CC adult females and males (at protein level) (PubMed:33060291).
CC Transcripts are expressed early in the life cycle and their expression
CC is maintained through the adult stage (PubMed:29739837).
CC {ECO:0000269|PubMed:29739837, ECO:0000269|PubMed:33060291}.
CC -!- DEVELOPMENTAL STAGE: [Isoform 2]: Is moderately detected in planulae
CC and a little more detected in primary polyps (9d), as well as in both
CC adult females and males (at protein level) (PubMed:33060291).
CC Transcripts are expressed early in the life cycle and their expression
CC is maintained through the adult stage (PubMed:29739837).
CC {ECO:0000269|PubMed:29739837, ECO:0000269|PubMed:33060291}.
CC -!- WEB RESOURCE: [Isoform 1]: Name=National Center for Biotechnology
CC Information (NCBI);
CC URL="https://www.ncbi.nlm.nih.gov/nuccore/HADN01006514.1/";
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DR EMBL; DS469766; EDO33598.1; -; Genomic_DNA.
DR RefSeq; XP_001625698.1; XM_001625648.1.
DR EnsemblMetazoa; EDO33598; EDO33598; NEMVEDRAFT_v1g233523. [A7SRR3-2]
DR HOGENOM; CLU_2415843_0_0_1; -.
DR Proteomes; UP000001593; Unassembled WGS sequence.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Alternative promoter usage; Cleavage on pair of basic residues;
KW Direct protein sequencing; Disulfide bond; Neuropeptide;
KW Reference proteome; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT PROPEP 24..51
FT /evidence="ECO:0000305|PubMed:33060291"
FT /id="PRO_0000453912"
FT CHAIN 52..92
FT /note="Neuropeptide ShK-like2"
FT /evidence="ECO:0000305|PubMed:33060291"
FT /id="PRO_0000453913"
FT DISULFID 53..92
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 61..85
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 70..89
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT VAR_SEQ 1..41
FT /note="MTTIRCVLFAVLLFAYCALLIKARSIDAEAEKTWQEEETKT -> MDVKLVA
FT ILFACTLFSLSFANGYRLESLRNLPNDALEEESE (in isoform 2)"
FT /evidence="ECO:0000269|PubMed:17615350"
FT /id="VSP_061205"
SQ SEQUENCE 92 AA; 10303 MW; FD803338586D0543 CRC64;
MTTIRCVLFA VLLFAYCALL IKARSIDAEA EKTWQEEETK TVAEKSPLKK RGCSDAFPVV
CRSPSVKAAC YNPNHRSHAF ITDVCKHTCH LC
