ID   SHKL4_NEMVE             Reviewed;          83 AA.
AC   A7ST80;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   23-FEB-2022, entry version 32.
DE   RecName: Full=Protein ShK-like4 {ECO:0000303|PubMed:33060291};
DE   Flags: Precursor;
OS   Nematostella vectensis (Starlet sea anemone).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Edwardsiidae; Nematostella.
OX   NCBI_TaxID=45351;
RN   [1] {ECO:0000312|EMBL:EDO33083.1, ECO:0000312|Proteomes:UP000001593}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CH2 X CH6 {ECO:0000312|Proteomes:UP000001593};
RX   PubMed=17615350; DOI=10.1126/science.1139158;
RA   Putnam N.H., Srivastava M., Hellsten U., Dirks B., Chapman J., Salamov A.,
RA   Terry A., Shapiro H., Lindquist E., Kapitonov V.V., Jurka J.,
RA   Genikhovich G., Grigoriev I.V., Lucas S.M., Steele R.E., Finnerty J.R.,
RA   Technau U., Martindale M.Q., Rokhsar D.S.;
RT   "Sea anemone genome reveals ancestral eumetazoan gene repertoire and
RT   genomic organization.";
RL   Science 317:86-94(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 31-43, IDENTIFICATION BY
RP   MASS SPECTROMETRY, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX   PubMed=33060291; DOI=10.1073/pnas.2011120117;
RA   Sachkova M.Y., Landau M., Surm J.M., Macrander J., Singer S.A.,
RA   Reitzel A.M., Moran Y.;
RT   "Toxin-like neuropeptides in the sea anemone Nematostella unravel
RT   recruitment from the nervous system to venom.";
RL   Proc. Natl. Acad. Sci. U.S.A. 117:27481-27492(2020).
RN   [3]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=29739837; DOI=10.1242/dev.162867;
RA   Warner J.F., Guerlais V., Amiel A.R., Johnston H., Nedoncelle K.,
RA   Roettinger E.;
RT   "NvERTx: a gene expression database to compare embryogenesis and
RT   regeneration in the sea anemone Nematostella vectensis.";
RL   Development 145:0-0(2018).
CC   -!- FUNCTION: Probable neuropeptide. {ECO:0000305|PubMed:33060291}.
CC   -!- TISSUE SPECIFICITY: Expressed in various neurons (ectodermal sensory
CC       cells) (in planulae and primary polyps). Not expressed in nematocytes.
CC       {ECO:0000269|PubMed:33060291}.
CC   -!- DEVELOPMENTAL STAGE: Very weakly detected in planulae, but moderately
CC       and equally detected in primary polyps (9d), and in both adult females
CC       and males (at protein level) (PubMed:33060291). Transcripts are
CC       expressed early in the life cycle and their expression is maintained
CC       through the adult stage (PubMed:29739837).
CC       {ECO:0000269|PubMed:29739837, ECO:0000269|PubMed:33060291}.
CC   -!- PTM: Contains 3 disulfide bonds. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; DS469791; EDO33083.1; -; Genomic_DNA.
DR   RefSeq; XP_001625183.1; XM_001625133.1.
DR   EnsemblMetazoa; EDO33083; EDO33083; NEMVEDRAFT_v1g233578.
DR   HOGENOM; CLU_2545286_0_0_1; -.
DR   Proteomes; UP000001593; Unassembled WGS sequence.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Neuropeptide; Reference proteome; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   PROPEP          22..48
FT                   /evidence="ECO:0000305|PubMed:33060291"
FT                   /id="PRO_0000453914"
FT   CHAIN           49..83
FT                   /note="Protein ShK-like4"
FT                   /evidence="ECO:0000305|PubMed:33060291"
FT                   /id="PRO_0000453915"
FT   DOMAIN          50..83
FT                   /note="ShKT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        50..82
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        57..75
FT                   /evidence="ECO:0000305"
FT   DISULFID        67..79
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
SQ   SEQUENCE   83 AA;  9259 MW;  A3B75044DC371B9A CRC64;
     MDTRVIAVLF VAIMVLSSTN ALPKQKGSYK NMNHADFLKG LDRASSKRDC RDSHWSCFFQ
     SNYEDICSTA QAEECALSCG LCE