ID   SHLD2_PONAB             Reviewed;         904 AA.
AC   Q5RCM2;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Shieldin complex subunit 2 {ECO:0000250|UniProtKB:Q86V20};
DE   AltName: Full=Protein FAM35A {ECO:0000250|UniProtKB:Q86V20};
DE   AltName: Full=RINN1-REV7-interacting novel NHEJ regulator 2;
GN   Name=SHLD2 {ECO:0000250|UniProtKB:Q86V20};
GN   Synonyms=FAM35A {ECO:0000250|UniProtKB:Q86V20},
GN   RINN2 {ECO:0000250|UniProtKB:Q86V20};
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the shieldin complex, which plays an important
CC       role in repair of DNA double-stranded breaks (DSBs). During G1 and S
CC       phase of the cell cycle, the complex functions downstream of TP53BP1 to
CC       promote non-homologous end joining (NHEJ) and suppress DNA end
CC       resection. Mediates various NHEJ-dependent processes including
CC       immunoglobulin class-switch recombination, and fusion of unprotected
CC       telomeres. {ECO:0000250|UniProtKB:Q86V20}.
CC   -!- SUBUNIT: Component of the shieldin complex, consisting of SHLD1, SHLD2,
CC       SHLD3 and MAD2L2/REV7. Within the complex, SHLD2 forms a scaffold which
CC       interacts with a SHLD3-MAD2L2 subcomplex via its N-terminus, and with
CC       SHLD1 via its C-terminus. Interacts with TP53BP1. Interacts with RIF1.
CC       {ECO:0000250|UniProtKB:Q86V20}.
CC   -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000250|UniProtKB:Q86V20}.
CC   -!- SIMILARITY: Belongs to the SHLD2 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CR858248; CAH90485.1; -; mRNA.
DR   RefSeq; NP_001125252.1; NM_001131780.1.
DR   AlphaFoldDB; Q5RCM2; -.
DR   STRING; 9601.ENSPPYP00000003293; -.
DR   GeneID; 100172149; -.
DR   KEGG; pon:100172149; -.
DR   CTD; 54537; -.
DR   eggNOG; ENOG502QW94; Eukaryota.
DR   InParanoid; Q5RCM2; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   InterPro; IPR029715; FAM35A.
DR   InterPro; IPR031589; FAM35A_C.
DR   PANTHER; PTHR14495; PTHR14495; 1.
DR   Pfam; PF15793; FAM35_C; 1.
PE   2: Evidence at transcript level;
KW   Chromosome; DNA damage; DNA repair; Reference proteome.
FT   CHAIN           1..904
FT                   /note="Shieldin complex subunit 2"
FT                   /id="PRO_0000087163"
FT   REGION          1..60
FT                   /note="Sufficient for interaction with SHLD3 and MAD2L2"
FT                   /evidence="ECO:0000250|UniProtKB:Q86V20"
FT   REGION          721..891
FT                   /note="Mediates interaction with SHLD1"
FT                   /evidence="ECO:0000250|UniProtKB:Q86V20"
SQ   SEQUENCE   904 AA;  101802 MW;  D109B0397AFEF45A CRC64;
     MSGGSQVHIF WGAPVAPLKM TVSQDTASLM SVADPWKKIH LLYSQHSLYL KDEKQHKNLE
     NYEVPESVGS PDFSGHFLAN CMNRHVHVKD DFVRSVSETQ NIESQNIHSS RLSDITSSNM
     QICGFKSTVP HLTEEEKYQK LLSENKIIDE QPKHQSDICG QNFNTNLFQL GHKCAAMLDL
     VCSTEQINIG PEVVQRECVS TEYHEIQNQC LGLFSSNAVD KSRSEAAVRK ASDLKISTDT
     EFLSIITSSQ VAFLAQKKDK GRSPINKGNV NMETEPKASY REIRIPEENS IQLDGFTEAY
     EGGQNQAYSL ELFSPVCRKT ENSRIHINSD KGLEEHIGSQ ELFNSEDKLP PNEIHIELCS
     SGILCSQLNT FHKSAIKRSC TSEDKVGQSE ALSRVLQVAK KMKLISNGGD SAVEMDRRNV
     SEFKGIKKTS LIKNCDSKSQ KYNCLVMVLS PCHVKEINIK FGPNSGSKVP LATVTVIDQS
     ETKKKVFLWR TAAFWAFTVF LGDIILLTDV VIREDQWVGE TVLQSTFSSQ LLNLGSYSSI
     QPEEYSSVVS DVVLQDLLAY VSSKHSYLRD LPPRQPQRVN SIDFVELEHL QPDVLVHAVL
     RVVDFTILTE AVYSYRGQKQ KKVMLTVEQA QDQHYVLVLW GPGAAWYPQL QRKKGYIWEF
     KYLFVQRNYT LENLELHTTP WSSCECLFND DIRAITFKAK FQKSAPSFVK IPDLATHLED
     KCSGVVLIQA QISELAFPIT AAQKIALNAH SSLKSIFSSL PNIVYTGCAK CGLELETDEN
     RIYKQCFSCL PFTMKKIYYR PALMTVVDGR HDVCIRVESK LIEKILLNIS ADCLNRVIVP
     SSEITYGMVV ADLFHSLLAV SAEPCVLKIQ SLFVLDENSY PLQQDFSLLD FYSDIVKHGA
     DARL