SHN3_ARATH
ID SHN3_ARATH Reviewed; 189 AA.
AC Q3E958; Q8GWK8;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Ethylene-responsive transcription factor SHINE 3;
GN Name=SHN3; Synonyms=ERF005; OrderedLocusNames=At5g25390;
GN ORFNames=F18G18.130;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=15319479; DOI=10.1105/tpc.104.022897;
RA Aharoni A., Dixit S., Jetter R., Thoenes E., van Arkel G., Pereira A.;
RT "The SHINE clade of AP2 domain transcription factors activates wax
RT biosynthesis, alters cuticle properties, and confers drought tolerance when
RT overexpressed in Arabidopsis.";
RL Plant Cell 16:2463-2480(2004).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
CC -!- FUNCTION: Promotes cuticle formation by inducing the expression of
CC enzymes involved in wax biosynthesis. Probably acts as a
CC transcriptional activator. Binds to the GCC-box pathogenesis-related
CC promoter element. May be involved in the regulation of gene expression
CC by stress factors and by components of stress signal transduction
CC pathways (By similarity). {ECO:0000250, ECO:0000269|PubMed:15319479}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3E958-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3E958-2; Sequence=VSP_027410;
CC -!- TISSUE SPECIFICITY: Found in all organs, mostly in veins, epidermis and
CC trichome bases. Specific expression in lateral root tips.
CC {ECO:0000269|PubMed:15319479}.
CC -!- INDUCTION: By wounding in cauline leaves, stems, and siliques, but not
CC in rosette leaves. {ECO:0000269|PubMed:15319479}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
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DR EMBL; AC006258; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED93434.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93435.1; -; Genomic_DNA.
DR EMBL; AK118780; BAC43373.1; -; mRNA.
DR EMBL; BT005461; AAO63881.1; -; mRNA.
DR EMBL; BX834028; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_197921.1; NM_122448.4. [Q3E958-1]
DR RefSeq; NP_851073.1; NM_180742.1. [Q3E958-2]
DR AlphaFoldDB; Q3E958; -.
DR SMR; Q3E958; -.
DR BioGRID; 17886; 13.
DR IntAct; Q3E958; 13.
DR STRING; 3702.AT5G25390.2; -.
DR PaxDb; Q3E958; -.
DR PRIDE; Q3E958; -.
DR ProteomicsDB; 234535; -. [Q3E958-1]
DR EnsemblPlants; AT5G25390.1; AT5G25390.1; AT5G25390. [Q3E958-2]
DR EnsemblPlants; AT5G25390.2; AT5G25390.2; AT5G25390. [Q3E958-1]
DR GeneID; 832611; -.
DR Gramene; AT5G25390.1; AT5G25390.1; AT5G25390. [Q3E958-2]
DR Gramene; AT5G25390.2; AT5G25390.2; AT5G25390. [Q3E958-1]
DR KEGG; ath:AT5G25390; -.
DR Araport; AT5G25390; -.
DR TAIR; locus:2145477; AT5G25390.
DR eggNOG; ENOG502RXNN; Eukaryota.
DR HOGENOM; CLU_042594_3_0_1; -.
DR InParanoid; Q3E958; -.
DR OMA; EDQMSMQ; -.
DR PhylomeDB; Q3E958; -.
DR PRO; PR:Q3E958; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q3E958; baseline and differential.
DR Genevisible; Q3E958; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; DNA-binding; Ethylene signaling pathway;
KW Nucleus; Reference proteome; Stress response; Transcription;
KW Transcription regulation.
FT CHAIN 1..189
FT /note="Ethylene-responsive transcription factor SHINE 3"
FT /id="PRO_0000297942"
FT DNA_BIND 6..63
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT VAR_SEQ 28..30
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11910074,
FT ECO:0000303|PubMed:14593172"
FT /id="VSP_027410"
SQ SEQUENCE 189 AA; 21254 MW; 97FF22990F8FDA1D CRC64;
MVHSKKFRGV RQRQWGSWVS EIRHPLLKRR VWLGTFDTAE TAARAYDQAA VLMNGQSAKT
NFPVIKSNGS NSLEINSALR SPKSLSELLN AKLRKNCKDQ TPYLTCLRLD NDSSHIGVWQ
KRAGSKTSPN WVKLVELGDK VNARPGGDIE TNKMKVRNED VQEDDQMAMQ MIEELLNWTC
PGSGSIAQV
