BH003_ORYSJ
ID BH003_ORYSJ Reviewed; 354 AA.
AC Q10S44; B9FAY4; F1DK91; Q0DVD5; Q8H8E4;
DT 10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Transcription factor BHLH3 {ECO:0000305};
DE AltName: Full=Basic helix-loop-helix protein 3 {ECO:0000303|PubMed:16896230};
DE Short=OsbHLH003 {ECO:0000303|PubMed:16896230};
DE AltName: Full=Protein RAC IMMUNITY 1 {ECO:0000303|PubMed:22437844};
DE AltName: Full=bHLH transcription factor 3 {ECO:0000305};
GN Name=BHLH3 {ECO:0000305};
GN Synonyms=BHLH003 {ECO:0000303|PubMed:16896230},
GN RAI1 {ECO:0000303|PubMed:22437844};
GN OrderedLocusNames=Os03g0135700 {ECO:0000312|EMBL:BAS82180.1},
GN LOC_Os03g04310 {ECO:0000312|EMBL:ABF93851.1};
GN ORFNames=OJ1006F06.1 {ECO:0000312|EMBL:AAN05491.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=18987217; DOI=10.1104/pp.108.128579;
RA Yilmaz A., Nishiyama M.Y., Fuentes B.G., Souza G.M., Janies D., Gray J.,
RA Grotewold E.;
RT "GRASSIUS: a platform for comparative regulatory genomics across the
RT grasses.";
RL Plant Physiol. 149:171-180(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16896230; DOI=10.1104/pp.106.080580;
RA Li X., Duan X., Jiang H., Sun Y., Tang Y., Yuan Z., Guo J., Liang W.,
RA Chen L., Yin J., Ma H., Wang J., Zhang D.;
RT "Genome-wide analysis of basic/helix-loop-helix transcription factor family
RT in rice and Arabidopsis.";
RL Plant Physiol. 141:1167-1184(2006).
RN [9]
RP FUNCTION, SUBUNIT, PHOSPHORYLATION, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=22437844; DOI=10.1093/pcp/pcs033;
RA Kim S.H., Oikawa T., Kyozuka J., Wong H.L., Umemura K., Kishi-Kaboshi M.,
RA Takahashi A., Kawano Y., Kawasaki T., Shimamoto K.;
RT "The bHLH Rac Immunity1 (RAI1) is activated by OsRac1 via OsMAPK3 and
RT OsMAPK6 in rice immunity.";
RL Plant Cell Physiol. 53:740-754(2012).
CC -!- FUNCTION: Transcription factor involved in defense responses that
CC functions downstream of RAC1 and upstream of PAL1 and WRKY19 genes.
CC {ECO:0000269|PubMed:22437844}.
CC -!- SUBUNIT: Interacts with LAX1. {ECO:0000269|PubMed:22437844}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:22437844}. Cytoplasm {ECO:0000269|PubMed:22437844}.
CC Note=Localizes mainly in the nucleus. {ECO:0000269|PubMed:22437844}.
CC -!- INDUCTION: Induced by sphingolipid elicitor and chitin elicitor, and a
CC compatible race of the rice blast fungus Magnaporthe oryzae.
CC {ECO:0000269|PubMed:22437844}.
CC -!- PTM: Phosphorylated by MAPK3 and MAPK6. {ECO:0000269|PubMed:22437844}.
CC -!- SIMILARITY: Belongs to the bHLH protein family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN05491.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF10803.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; HQ858849; ADX60261.1; -; mRNA.
DR EMBL; AC099399; AAN05491.1; ALT_SEQ; Genomic_DNA.
DR EMBL; DP000009; ABF93851.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF10803.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014959; BAS82180.1; -; Genomic_DNA.
DR EMBL; CM000140; EEE58284.1; -; Genomic_DNA.
DR EMBL; AK060029; BAG87280.1; -; mRNA.
DR RefSeq; XP_015629094.1; XM_015773608.1.
DR AlphaFoldDB; Q10S44; -.
DR SMR; Q10S44; -.
DR STRING; 4530.OS03T0135700-01; -.
DR PaxDb; Q10S44; -.
DR PRIDE; Q10S44; -.
DR EnsemblPlants; Os03t0135700-01; Os03t0135700-01; Os03g0135700.
DR EnsemblPlants; Os03t0135700-02; Os03t0135700-02; Os03g0135700.
DR GeneID; 4331538; -.
DR Gramene; Os03t0135700-01; Os03t0135700-01; Os03g0135700.
DR Gramene; Os03t0135700-02; Os03t0135700-02; Os03g0135700.
DR KEGG; osa:4331538; -.
DR eggNOG; ENOG502QQHH; Eukaryota.
DR HOGENOM; CLU_035660_1_2_1; -.
DR OMA; QHPHEEF; -.
DR OrthoDB; 1023623at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0031347; P:regulation of defense response; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Nucleus; Plant defense; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..354
FT /note="Transcription factor BHLH3"
FT /id="PRO_0000452037"
FT DOMAIN 178..227
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 124..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 178..191
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 192..227
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT CONFLICT 319
FT /note="G -> D (in Ref. 1; ADX60261)"
FT /evidence="ECO:0000305"
FT CONFLICT 339
FT /note="I -> N (in Ref. 1; ADX60261)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 354 AA; 37890 MW; 33FD84A6FD31A39D CRC64;
MELDEESFLD ELMSLRRDGS APWQAPPYPG GGGGGGGGGM MMSDLLFYGG DGGSAEARGG
MDASPFQELA SMAAPPPQHP HEEFNFDCLS EVCNPYRSCG AQLVPSEAAS QTQTQLTPLR
DAMVAEEETS GDKALLHGGG GSSSPTFMFG GGAGESSEMM AGIRGVGGGV HPRSKLHGTP
SKNLMAERRR RKRLNDRLSM LRSIVPKISK MDRTSILGDT IDYVKELTER IKTLEEEIGV
TPEELDLLNT MKDSSSGNNN EMLVRNSTKF DVENRGSGNT RIEICCPANP GVLLSTVSAL
EVLGLEIEQC VVSCFSDFGM QASCLQEDGK RQVVSTDEIK QTLFRSAGYG GRCL
