BH006_ORYSJ
ID BH006_ORYSJ Reviewed; 310 AA.
AC Q0JEB7; A0A0P0W8T9; Q7XMC8; Q84LF9;
DT 10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Transcription factor BHLH6 {ECO:0000305};
DE AltName: Full=Basic helix-loop-helix protein 6 {ECO:0000303|PubMed:16896230};
DE Short=OsbHLH6 {ECO:0000303|PubMed:33128314};
DE Short=osbHLH006 {ECO:0000303|PubMed:16896230};
DE AltName: Full=bHLH transcription factor 6 {ECO:0000305};
GN Name=BHLH6 {ECO:0000303|PubMed:33128314};
GN Synonyms=BHLH006 {ECO:0000303|PubMed:16896230},
GN RERJ1 {ECO:0000303|PubMed:15541369};
GN OrderedLocusNames=Os04g0301500 {ECO:0000312|EMBL:BAS88467.1},
GN LOC_Os04g23550 {ECO:0000305};
GN ORFNames=OSJNBb0018A10.7 {ECO:0000312|EMBL:CAE04678.3};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INDUCTION BY JASMONATE.
RX PubMed=15541369; DOI=10.1016/j.bbrc.2004.10.126;
RA Kiribuchi K., Sugimori M., Takeda M., Otani T., Okada K., Onodera H.,
RA Ugaki M., Tanaka Y., Tomiyama-Akimoto C., Yamaguchi T., Minami E.,
RA Shibuya N., Omori T., Nishiyama M., Nojiri H., Yamane H.;
RT "RERJ1, a jasmonic acid-responsive gene from rice, encodes a basic helix-
RT loop-helix protein.";
RL Biochem. Biophys. Res. Commun. 325:857-863(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RG The rice full-length cDNA consortium;
RT "Oryza sativa full length cDNA.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP INDUCTION.
RX PubMed=15914931; DOI=10.1271/bbb.69.1042;
RA Kiribuchi K., Jikumaru Y., Kaku H., Minami E., Hasegawa M., Kodama O.,
RA Seto H., Okada K., Nojiri H., Yamane H.;
RT "Involvement of the basic helix-loop-helix transcription factor RERJ1 in
RT wounding and drought stress responses in rice plants.";
RL Biosci. Biotechnol. Biochem. 69:1042-1044(2005).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16896230; DOI=10.1104/pp.106.080580;
RA Li X., Duan X., Jiang H., Sun Y., Tang Y., Yuan Z., Guo J., Liang W.,
RA Chen L., Yin J., Ma H., Wang J., Zhang D.;
RT "Genome-wide analysis of basic/helix-loop-helix transcription factor family
RT in rice and Arabidopsis.";
RL Plant Physiol. 141:1167-1184(2006).
RN [9]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=22456953; DOI=10.1007/s00709-012-0400-z;
RA Miyamoto K., Shimizu T., Mochizuki S., Nishizawa Y., Minami E., Nojiri H.,
RA Yamane H., Okada K.;
RT "Stress-induced expression of the transcription factor RERJ1 is tightly
RT regulated in response to jasmonic acid accumulation in rice.";
RL Protoplasma 250:241-249(2013).
RN [10]
RP FUNCTION, INTERACTION WITH SPX4, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND INDUCTION.
RX PubMed=33128314; DOI=10.1111/tpj.15061;
RA He Q., Lu H., Guo H., Wang Y., Zhao P., Li Y., Wang F., Xu J., Mo X.,
RA Mao C.;
RT "OsbHLH6 interacts with OsSPX4 and regulates the phosphate-starvation
RT response in rice.";
RL Plant J. 105:649-667(2021).
CC -!- FUNCTION: Transcription factor involved in phosphate starvation
CC response (PubMed:33128314). Acts as positive regulator of phosphate
CC homeostasis and phosphate signaling by antagonizing SPX4
CC (PubMed:33128314). Transcription factor involved jasmonate signaling
CC (PubMed:15541369, PubMed:22456953). Regulates shoot growth inhibition
CC in response to jasmonate (PubMed:15541369). May play a role as
CC transcriptional activator in the regulation of jasmonate-mediated
CC stress-inducible gene expression (PubMed:22456953). Possesses
CC transactivation activity in vivo (PubMed:22456953, PubMed:33128314).
CC {ECO:0000269|PubMed:15541369, ECO:0000269|PubMed:22456953,
CC ECO:0000269|PubMed:33128314}.
CC -!- SUBUNIT: Forms homodimers (PubMed:33128314). Interacts with SPX4
CC (PubMed:33128314). {ECO:0000269|PubMed:33128314}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:33128314}. Nucleus
CC {ECO:0000255|PROSITE-ProRule:PRU00981, ECO:0000269|PubMed:22456953,
CC ECO:0000269|PubMed:33128314}.
CC -!- TISSUE SPECIFICITY: Expressed in spikelets, leaf mesophyll cells, root
CC tip and root epidermis cells. {ECO:0000269|PubMed:33128314}.
CC -!- INDUCTION: Induced by jasmonate (PubMed:15541369, PubMed:15914931,
CC PubMed:22456953). Induced by wounding and drought stress in leaves
CC (PubMed:15914931, PubMed:22456953). Induced by phosphate starvation in
CC shoots (PubMed:33128314). {ECO:0000269|PubMed:15541369,
CC ECO:0000269|PubMed:15914931, ECO:0000269|PubMed:22456953,
CC ECO:0000269|PubMed:33128314}.
CC -!- MISCELLANEOUS: Overexpression of BHLH6 promotes shoot and root
CC phosphate accumulation, and root hair growth and acid phosphatase
CC activity under phosphate sufficiency. {ECO:0000269|PubMed:33128314}.
CC -!- SIMILARITY: Belongs to the bHLH protein family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAE04678.3; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB040744; BAC66785.1; -; mRNA.
DR EMBL; AL662999; CAE04678.3; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008210; BAF14320.1; -; Genomic_DNA.
DR EMBL; AP014960; BAS88467.1; -; Genomic_DNA.
DR EMBL; AK242210; BAH01225.1; -; mRNA.
DR RefSeq; XP_015634154.1; XM_015778668.1.
DR AlphaFoldDB; Q0JEB7; -.
DR SMR; Q0JEB7; -.
DR STRING; 4530.OS04T0301500-01; -.
DR PaxDb; Q0JEB7; -.
DR PRIDE; Q0JEB7; -.
DR EnsemblPlants; Os04t0301500-01; Os04t0301500-01; Os04g0301500.
DR GeneID; 4335417; -.
DR Gramene; Os04t0301500-01; Os04t0301500-01; Os04g0301500.
DR KEGG; osa:4335417; -.
DR eggNOG; ENOG502QWG4; Eukaryota.
DR HOGENOM; CLU_035660_0_0_1; -.
DR InParanoid; Q0JEB7; -.
DR OMA; EHEYGHH; -.
DR OrthoDB; 1160318at2759; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0009867; P:jasmonic acid mediated signaling pathway; IDA:UniProtKB.
DR GO; GO:0055062; P:phosphate ion homeostasis; IMP:UniProtKB.
DR GO; GO:0080040; P:positive regulation of cellular response to phosphate starvation; IMP:UniProtKB.
DR GO; GO:0048831; P:regulation of shoot system development; IDA:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR045084; AIB/MYC-like.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR PANTHER; PTHR11514; PTHR11514; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR SUPFAM; SSF55021; SSF55021; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Jasmonic acid signaling pathway; Nucleus;
KW Reference proteome; Stress response; Transcription;
KW Transcription regulation.
FT CHAIN 1..310
FT /note="Transcription factor BHLH6"
FT /id="PRO_0000452038"
FT DOMAIN 89..138
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 26..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 89..102
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 103..138
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 174..194
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 271
FT /note="L -> P (in Ref. 1; BAC66785)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 310 AA; 32948 MW; 92EA218846FBF385 CRC64;
MDAEMAMGES FAYYWETQRY LESEELDSMY LPTQDDSNYE SSSPDGSHSS SAPAPAAVGG
DAAAAVAGSG GGMTTMMMGG GGGGGDDAGG ANKNILMERD RRRKLNEKLY ALRSVVPNIT
KMDKASIIKD AIEYIQRLQA EEQQMLREVA ALESAAAASA APAAANPFAG LGADEEHEYG
HHHPSSSSER TKKVKRALSV SSISDALLAA AAPAPPVEIQ ELRVSEVGDR VLVVSVTCSK
RRDAMARVCR ALEELRLRVI TANITSVAGC LMHTLFVEVD HMDSVQMKQM VEAALSQLVA
TGSPLSSMSY
