SHOB_ECOLI
ID SHOB_ECOLI Reviewed; 26 AA.
AC C1P611;
DT 13-OCT-2009, integrated into UniProtKB/Swiss-Prot.
DT 26-MAY-2009, sequence version 1.
DT 03-AUG-2022, entry version 44.
DE RecName: Full=Small toxic protein ShoB;
GN Name=shoB; Synonyms=ryfB, yphI; OrderedLocusNames=b4687, JW2546.1;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP IDENTIFICATION, INDUCTION, AND OVEREXPRESSION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=18710431; DOI=10.1111/j.1365-2958.2008.06394.x;
RA Fozo E.M., Kawano M., Fontaine F., Kaya Y., Mendieta K.S., Jones K.L.,
RA Ocampo A., Rudd K.E., Storz G.;
RT "Repression of small toxic protein synthesis by the Sib and OhsC small
RT RNAs.";
RL Mol. Microbiol. 70:1076-1093(2008).
CC -!- FUNCTION: Toxic component of a type I toxin-antitoxin (TA) system. May
CC be a toxic protein; overexpression causes cessation of growth and rapid
CC membrane depolarization. Overexpression induces stress-response and a
CC number of membrane protein genes. {ECO:0000269|PubMed:18710431}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: In exponential phase. The ohsC RNA (previously known as
CC ryfC) prevents the toxic effects of shoB overproduction, probably by
CC repressing its translation. Expression of the proteinaceous toxin is
CC controlled by antisense sRNA OhsC. {ECO:0000269|PubMed:18710431}.
CC -!- MISCELLANEOUS: Entirely encoded within a small RNA previously known as
CC ryfB. {ECO:0000305}.
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DR EMBL; U00096; ACO60003.1; -; Genomic_DNA.
DR EMBL; AP009048; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; WP_000128776.1; NZ_STEB01000011.1.
DR RefSeq; YP_002791251.1; NC_000913.3.
DR AlphaFoldDB; C1P611; -.
DR STRING; 511145.b4687; -.
DR TCDB; 1.C.135.1.1; the shob pore-forming toxin (shob) family.
DR PaxDb; C1P611; -.
DR PRIDE; C1P611; -.
DR EnsemblBacteria; ACO60003; ACO60003; b4687.
DR GeneID; 66673549; -.
DR GeneID; 7751620; -.
DR KEGG; eco:b4687; -.
DR PATRIC; fig|83333.103.peg.3447; -.
DR BioCyc; EcoCyc:MON0-2860; -.
DR PRO; PR:C1P611; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0012501; P:programmed cell death; IMP:EcoCyc.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Toxin-antitoxin system; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..26
FT /note="Small toxic protein ShoB"
FT /id="PRO_0000386426"
FT TRANSMEM 7..24
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 26 AA; 3103 MW; 9778AB7EA9619298 CRC64;
MTDCRYLIKR VIKIIIAVLQ LILLFL
